BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0228.0025.p
         (121 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300311759|ref|YP_003775851.1| short-chain alcohol dehydrogena...   146   4e-37
gi|300311727|ref|YP_003775819.1| dehydrogenase [Herbaspirillum s...   132   6e-33
gi|300311620|ref|YP_003775712.1| short chain dehydrogenase/reduc...    59   1e-10
gi|300310554|ref|YP_003774646.1| short-chain alcohol dehydrogena...    54   4e-09

>gi|300311759|ref|YP_003775851.1| short-chain alcohol dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 247

 Score =  146 bits (369), Expect = 4e-37,   Method: Compositional matrix adjust.
 Identities = 72/105 (68%), Positives = 85/105 (80%)

Query: 1   LGALEGLPMDSVREIFDTNTFGTMAMIQAVLPQFRRRMAGVIVNVTSSVTLKPLPLLSVY 60
           LGALE  PM  VRE+F+TNT G++AM QA +PQ R R  G+++NVTSSVTL+ LPLL+VY
Sbjct: 81  LGALEAQPMAQVRELFETNTLGSIAMTQAFIPQMRARGQGLVINVTSSVTLRALPLLAVY 140

Query: 61  TASKAAVNAFTESLALELKQFNVRVSLVLPGRAPSTRFGENARPR 105
           TASKAA+NAFTESLALEL    V+V LVLPGR+P TRF ENAR R
Sbjct: 141 TASKAAINAFTESLALELAPLGVQVRLVLPGRSPQTRFAENARAR 185


>gi|300311727|ref|YP_003775819.1| dehydrogenase [Herbaspirillum
           seropedicae SmR1]
          Length = 247

 Score =  132 bits (333), Expect = 6e-33,   Method: Compositional matrix adjust.
 Identities = 66/108 (61%), Positives = 74/108 (68%)

Query: 1   LGALEGLPMDSVREIFDTNTFGTMAMIQAVLPQFRRRMAGVIVNVTSSVTLKPLPLLSVY 60
            GA E  PM +VREIF+TNTFG MAM QAV+PQFR R  GVI+NVTSS TL   PL++ Y
Sbjct: 81  FGAFEATPMSTVREIFETNTFGVMAMCQAVIPQFRERGDGVIINVTSSATLAAFPLVAAY 140

Query: 61  TASKAAVNAFTESLALELKQFNVRVSLVLPGRAPSTRFGENARPRXGG 108
           TASK A+  FT SLA ELK   VRV LV PG  PST F  N + R  G
Sbjct: 141 TASKTAIEGFTASLAHELKAVGVRVKLVEPGYGPSTSFATNGQQRMQG 188


>gi|300311620|ref|YP_003775712.1| short chain
           dehydrogenase/reductase [Herbaspirillum seropedicae
           SmR1]
          Length = 339

 Score = 58.5 bits (140), Expect = 1e-10,   Method: Compositional matrix adjust.
 Identities = 37/106 (34%), Positives = 57/106 (53%), Gaps = 5/106 (4%)

Query: 1   LGALEGLPMDSVREIFDTNTFGTMAMIQAVLPQFRRRMAG-VIVNVTSSVTLKPLPLLSV 59
            G  E + ++  R +F+TN +G +    A LP  +    G V++N+ S ++   +PL   
Sbjct: 100 FGRHEEVSLEDHRRLFETNYWGVVNGSLAALPHLKANPEGAVLINLGSELSDVAVPLQGA 159

Query: 60  YTASKAAVNAFTESLALELKQFNVRVSLVLPGRA----PSTRFGEN 101
           Y+ASK AV  FT+SL +EL +   RVS+ L   A    P TR  +N
Sbjct: 160 YSASKHAVKGFTDSLRVELAEQGARVSVTLVKPAAIDTPFTRHAKN 205


>gi|300310554|ref|YP_003774646.1| short-chain alcohol dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 250

 Score = 53.9 bits (128), Expect = 4e-09,   Method: Compositional matrix adjust.
 Identities = 32/98 (32%), Positives = 48/98 (48%)

Query: 1   LGALEGLPMDSVREIFDTNTFGTMAMIQAVLPQFRRRMAGVIVNVTSSVTLKPLPLLSVY 60
           LG    + +     + DTN  G + +  A+LP   +R  G I+N+ S     P P  +VY
Sbjct: 89  LGPAHEVALSDWETMIDTNVKGLVTITHALLPDMVKRGRGTIINLGSVAGAYPYPGGNVY 148

Query: 61  TASKAAVNAFTESLALELKQFNVRVSLVLPGRAPSTRF 98
            A+KA V+ FT +L  +L    VR + + PG    T F
Sbjct: 149 GATKAFVDQFTLNLRADLVGTGVRATNLAPGLCGGTEF 186


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.322    0.135    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 278,254
Number of extensions: 8592
Number of successful extensions: 87
Number of sequences better than 1.0e-08: 4
Number of HSP's gapped: 97
Number of HSP's successfully gapped: 4
Length of query: 121
Length of database: 1,619,870
Length adjustment: 74
Effective length of query: 47
Effective length of database: 1,269,480
Effective search space: 59665560
Effective search space used: 59665560
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 125 (52.8 bits)