BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0228.0024.p (102 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311759|ref|YP_003775851.1| short-chain alcohol dehydrogena... 100 4e-23 gi|300311727|ref|YP_003775819.1| dehydrogenase [Herbaspirillum s... 85 1e-18 >gi|300311759|ref|YP_003775851.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 247 Score = 99.8 bits (247), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 46/62 (74%), Positives = 50/62 (80%) Query: 10 MKTVLITGCSSGFGLETAQYFLDRDWNIIATMRTPREDLLPRSERLRILALDVTDADSIR 69 MKT+ ITGCSSGFGLE A+YFL RDW +IATMRTPR DLLP S LRIL LDV DA SI+ Sbjct: 1 MKTIFITGCSSGFGLEIARYFLQRDWKVIATMRTPRHDLLPASPHLRILPLDVADAHSIQ 60 Query: 70 RA 71 A Sbjct: 61 AA 62 >gi|300311727|ref|YP_003775819.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 247 Score = 85.1 bits (209), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 40/62 (64%), Positives = 49/62 (79%) Query: 10 MKTVLITGCSSGFGLETAQYFLDRDWNIIATMRTPREDLLPRSERLRILALDVTDADSIR 69 M TVLITGCSSG+GLETA++FL W +IATMR+PR DLL S+ L IL LDV+ DSI+ Sbjct: 1 MNTVLITGCSSGYGLETARHFLANGWKVIATMRSPRFDLLSPSDNLCILPLDVSQPDSIK 60 Query: 70 RA 71 +A Sbjct: 61 QA 62 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.141 0.445 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 323,770 Number of extensions: 11604 Number of successful extensions: 62 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 62 Number of HSP's successfully gapped: 2 Length of query: 102 Length of database: 1,619,870 Length adjustment: 71 Effective length of query: 31 Effective length of database: 1,283,685 Effective search space: 39794235 Effective search space used: 39794235 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 123 (52.0 bits)