BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0228.0017.p (178 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313913|ref|YP_003778005.1| short-chain dehydrogenase [Herb... 146 8e-37 gi|300311759|ref|YP_003775851.1| short-chain alcohol dehydrogena... 70 1e-13 gi|300310554|ref|YP_003774646.1| short-chain alcohol dehydrogena... 64 7e-12 gi|300311727|ref|YP_003775819.1| dehydrogenase [Herbaspirillum s... 57 1e-09 gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herba... 56 1e-09 >gi|300313913|ref|YP_003778005.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 270 Score = 146 bits (369), Expect = 8e-37, Method: Compositional matrix adjust. Identities = 79/174 (45%), Positives = 108/174 (62%) Query: 1 VNNAGIARLAPIGGSALQDSLSQIALNITAMTRLTHAVLPGLKARNEGLIINISSALALH 60 VNNAGI P+ S ++ IALN+TA TRLT+A +PG AR +G +INISS + + Sbjct: 92 VNNAGIGTHTPLLQSDVEHMTGMIALNVTAPTRLTYAAVPGFVARGQGAVINISSIVGVA 151 Query: 61 SLPISAVYSGTKAFVLSFSRGLQQELADSAVKVQVVLPAATATEVWDQSGVPLSALNPDT 120 ++ VY G+KAFVL+FS+ L ELA V+VQ VLP ATAT+ W G+P+ L+P Sbjct: 152 PEVLNGVYGGSKAFVLAFSQSLHHELAAQGVQVQAVLPGATATDFWAIGGLPVEHLDPAI 211 Query: 121 VMPTEAMVDAAMTALDQGESITLPAVADAGAIERFESARAALFADTQTGKVAPR 174 VM E MVDAA+ + E +T+P++ + A +E+AR AL A APR Sbjct: 212 VMSAEQMVDAALVGFQRRELVTIPSLHEEDAWTGYEAARQALIARLSANTPAPR 265 >gi|300311759|ref|YP_003775851.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 247 Score = 69.7 bits (169), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 36/98 (36%), Positives = 58/98 (59%) Query: 1 VNNAGIARLAPIGGSALQDSLSQIALNITAMTRLTHAVLPGLKARNEGLIINISSALALH 60 VNNAGI L + + N +T A +P ++AR +GL+IN++S++ L Sbjct: 73 VNNAGIGNLGALEAQPMAQVRELFETNTLGSIAMTQAFIPQMRARGQGLVINVTSSVTLR 132 Query: 61 SLPISAVYSGTKAFVLSFSRGLQQELADSAVKVQVVLP 98 +LP+ AVY+ +KA + +F+ L ELA V+V++VLP Sbjct: 133 ALPLLAVYTASKAAINAFTESLALELAPLGVQVRLVLP 170 >gi|300310554|ref|YP_003774646.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 250 Score = 63.9 bits (154), Expect = 7e-12, Method: Compositional matrix adjust. Identities = 36/100 (36%), Positives = 52/100 (52%), Gaps = 1/100 (1%) Query: 1 VNNAGIA-RLAPIGGSALQDSLSQIALNITAMTRLTHAVLPGLKARNEGLIINISSALAL 59 +NNAG+A L P AL D + I N+ + +THA+LP + R G IIN+ S Sbjct: 80 INNAGLALGLGPAHEVALSDWETMIDTNVKGLVTITHALLPDMVKRGRGTIINLGSVAGA 139 Query: 60 HSLPISAVYSGTKAFVLSFSRGLQQELADSAVKVQVVLPA 99 + P VY TKAFV F+ L+ +L + V+ + P Sbjct: 140 YPYPGGNVYGATKAFVDQFTLNLRADLVGTGVRATNLAPG 179 >gi|300311727|ref|YP_003775819.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 247 Score = 56.6 bits (135), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 29/98 (29%), Positives = 49/98 (50%) Query: 1 VNNAGIARLAPIGGSALQDSLSQIALNITAMTRLTHAVLPGLKARNEGLIINISSALALH 60 VNNAGI + + N + + AV+P + R +G+IIN++S+ L Sbjct: 73 VNNAGIGLFGAFEATPMSTVREIFETNTFGVMAMCQAVIPQFRERGDGVIINVTSSATLA 132 Query: 61 SLPISAVYSGTKAFVLSFSRGLQQELADSAVKVQVVLP 98 + P+ A Y+ +K + F+ L EL V+V++V P Sbjct: 133 AFPLVAAYTASKTAIEGFTASLAHELKAVGVRVKLVEP 170 >gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 253 Score = 56.2 bits (134), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 37/116 (31%), Positives = 62/116 (53%), Gaps = 6/116 (5%) Query: 1 VNNAG-IARLAPIGGSALQDSLSQIALNITA--MTRLTHAVLPGLKARNEGLIINISSAL 57 +NNAG + + AP+ + D L +NI A + T AV+P ++A+ G IIN++S Sbjct: 89 INNAGALVKRAPL--ETVTDELFDDIININARSVVAFTRAVIPVMRAQGGGNIINVTSVA 146 Query: 58 ALHSL-PISAVYSGTKAFVLSFSRGLQQELADSAVKVQVVLPAATATEVWDQSGVP 112 A H P + +Y+ +K FV + +RG+ +EL ++V V P T ++ P Sbjct: 147 ARHGGGPGALIYAASKGFVSTLTRGMAKELLADKIRVNAVAPGVIMTPFQERFTTP 202 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.128 0.346 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 533,979 Number of extensions: 20581 Number of successful extensions: 139 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 150 Number of HSP's successfully gapped: 9 Length of query: 178 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 99 Effective length of database: 1,245,805 Effective search space: 123334695 Effective search space used: 123334695 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 127 (53.5 bits)