BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0228.0016.p (334 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312794|ref|YP_003776886.1| NADPH:quinone oxidoreductase [H... 138 6e-34 gi|300310813|ref|YP_003774905.1| NADPH:quinone oxidoreductase [H... 124 1e-29 gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [H... 108 8e-25 gi|300312848|ref|YP_003776940.1| NADPH:quinone oxidoreductase [H... 91 1e-19 gi|300310417|ref|YP_003774509.1| zinc-containing alcohol dehydro... 79 6e-16 >gi|300312794|ref|YP_003776886.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 342 Score = 138 bits (347), Expect = 6e-34, Method: Compositional matrix adjust. Identities = 111/357 (31%), Positives = 166/357 (46%), Gaps = 49/357 (13%) Query: 1 MKALVLKSYGGLDKVEFADISRPAINPDEILVQVLAAGLNPIDYMIPKGMFKPIMP---- 56 MKA+ ++GG + + + D P + P E+LV+V A GLNP D+ + +G +K + P Sbjct: 6 MKAIQQHAFGGPEVLRYEDAPVPELQPGEVLVRVHAVGLNPPDWYLREG-YKMLPPEWQP 64 Query: 57 -LQLPHTVGSDLAGIVVETGSRVTRFKVGDAVFASI---FDLPGGS--LAEFASVPEYAA 110 + P +G+D++G+V V F VGD V++ + L GGS A++ SV Sbjct: 65 TVAFPLILGTDISGVVEAVADDVKDFAVGDEVYSMVRFPSGLAGGSRAYAQYVSVAASEI 124 Query: 111 ALKPANLDFVQAASIPMVGLTSWQAL---------------HERMQLRPGQKVFIPAGSG 155 ALKP + QAA+ PM LT+WQ + HE + L+ + V + +G Sbjct: 125 ALKPQRISHAQAAAAPMSLLTAWQFMIELGHDIQNPLQPHRHEPVPLK-DKTVVVNGAAG 183 Query: 156 GIGTFAIQLAKHLGATVGTTTSSVNVDLVRSLGADVVVDYKKQHVEQVLQGYDAVLGTIR 215 G+G FA+QLAK GA V S + +R LGAD +DY + E VL D V+ + Sbjct: 184 GVGHFALQLAKWKGARVIAIGSGGHEAFLRDLGADEFIDYTRSAAEDVLSDVDLVIDAVG 243 Query: 216 GDALEKSLAILQPGSKVVSLIGPPDAAFARARGMNFFMKIVFGLLSAKIIRRAKKHDASY 275 G A + L L+ RG F G A+ A + + Sbjct: 244 GPASGRFLRTLK-------------------RGGALFPIFPLGFSGAE---EAARLGVNV 281 Query: 276 SFLFLRPDGRQLAQIGDLLATGGIRPVIDKVFAFDQAKDGLAYLEQGRAKGKVVVQM 332 S +R G QL Q+ LL +G +R ID FA A QG +GK+V+ + Sbjct: 282 STTQVRSSGAQLQQLAGLLDSGVVRVAIDSTFALQDAGKAHERAAQGHIRGKIVLSV 338 >gi|300310813|ref|YP_003774905.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 309 Score = 124 bits (311), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 98/336 (29%), Positives = 156/336 (46%), Gaps = 36/336 (10%) Query: 1 MKALVLKSYGGLDKVEFADISRPAINPDEILVQVLAAGLNPIDYMIPKGMFKPIMPLQLP 60 M+A+ YG + D+ + P + V + AAG+ P+D + G + ++ L LP Sbjct: 1 MRAICYHQYGPPSVLHLCDLDEQPLPPGHVRVAMQAAGVAPVDTKLRAGRLQHLVALDLP 60 Query: 61 HTVGSDLAGIVVETGSRVTRFKVGDAVFASIFDLP-----GGSLAEFASVPEYAAALKPA 115 G D AG++ G V +VG+AV +P G +LAE +P+ KP Sbjct: 61 KVPGRDGAGVITALGEGVHTLQVGEAVCV----MPSMAGRGTTLAELV-LPQEQVVRKPD 115 Query: 116 NLDFVQAASIPMVGLTSWQALHERMQLRPGQKVFIPAGSGGIGTFAIQLAKHLGATVGTT 175 L QAA++ ++W AL + + G KVFI AGSG +G+ +QLA HLGA V + Sbjct: 116 VLSMQQAAALLQPACSAWIALMQTAPIAAGMKVFIHAGSGAVGSLMVQLAAHLGAEVTAS 175 Query: 176 TSSVNVDLVRSLGADVVVDYKKQHVEQVLQGYDAVLGTIRGDALEKSLAILQPGSKVVSL 235 S NV+ V +LGA V+ Y + + +V + D V+ + G A ++S +L+ G ++V L Sbjct: 176 CRSSNVEYVAALGASRVLAYDRDELGRV-RDQDVVIDLVGGAAHDQSYRMLRRGGQLVWL 234 Query: 236 IGPPDAAFARARGMNFFMKIVFGLLSAKIIRRAKKHDASYSFLFLRPDGRQLAQIGDLLA 295 + P R RG F + +RRA + D L Q+ L Sbjct: 235 VAAP----IRERGEEFGVS----------VRRA----------LIVEDQSILQQVAALAQ 270 Query: 296 TGGIRPVIDKVFAFDQAKDGLAYLEQGR-AKGKVVV 330 G RP + V + +E G +G++V+ Sbjct: 271 QGVWRPAVSGVLPMQEIARAHQRIEAGEVTRGRLVL 306 >gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 325 Score = 108 bits (269), Expect = 8e-25, Method: Compositional matrix adjust. Identities = 99/348 (28%), Positives = 162/348 (46%), Gaps = 39/348 (11%) Query: 1 MKALVLKSYGGLDKVEFADISRPAINPDEILVQVLAAGLNPIDYMIPKGMFKPIMP--LQ 58 M+A+ +K +GG + ++ + P + E+L++V AAG+N D G + P+ P Sbjct: 1 MRAIEIKEFGGPEVLQACERPVPELKAGEVLIKVHAAGVNRPDVFQRTGNY-PVPPGASD 59 Query: 59 LPHTVGSDLAGIVVETGSRVTRFKVGDAVFASIFDLPGGSLAEFASVPEYAAALKPANLD 118 LP G ++AG +V + FK+GD V A + GG AEF + P P L Sbjct: 60 LP---GLEVAGEIVAGDLGDSGFKLGDLVCALV---QGGGYAEFCAAPLAQCLPVPKGLS 113 Query: 119 FVQAASIPMVGLTSWQALHERMQLRPGQKVFIPAGSGGIGTFAIQLAKHLGATV-GTTTS 177 ++AA++P T W + +R QL + + + GS GIG AIQ+A+ LG V T S Sbjct: 114 ALEAAALPENYFTVWSNVFDRGQLSGEETLLVQGGSSGIGVTAIQIARALGHRVFATAGS 173 Query: 178 SVNVDLVRSLGADVVVDYKKQHVEQVLQ------GYDAVLGTIRGDALEKSLAILQPGSK 231 + LGA+ ++YK + E V++ G D +L + GD L + + L + Sbjct: 174 AEKCRACEELGAERAINYKTEDFEAVVKELTGGRGVDVILDMVGGDYLPREIKALADDGR 233 Query: 232 VVSLIGPPDAAFARARGMNFFMKIVFGLLSAKIIRRAKKHDASYSFLFLRPDGRQLA--- 288 +V + A A+G L +++RR + + S L RP + A Sbjct: 234 LVFI-----AQLGGAKGQ---------LDMGQVMRR--RLTITGSTLRPRPVAFKAAIAA 277 Query: 289 ----QIGDLLATGGIRPVIDKVFAFDQAKDGLAYLEQGRAKGKVVVQM 332 + L+ G IRPVI F +QA A +E GK+++++ Sbjct: 278 KLREHVWPLIEAGKIRPVIHHRFPLEQAAQAHAMMEGSTHIGKIMLEL 325 >gi|300312848|ref|YP_003776940.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 324 Score = 91.3 bits (225), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 74/241 (30%), Positives = 115/241 (47%), Gaps = 13/241 (5%) Query: 2 KALVLKSYGGLDKVEFADISRPAINPDEILVQVLAAGLNPIDYMIPKGMFKPIMPLQLPH 61 KA+ + GG + +E+ D+ E+ ++ A GLN ID G++ P LP Sbjct: 3 KAIRMSKTGGPEVMEYVDVEVGEPGAGEVRIRHAAVGLNFIDCYFRAGLY----PQPLPA 58 Query: 62 TVGSDLAGIVVETGSRVTRFKVGDAVFASIFDLPGGSLAEFASVPEYAAALKPANLDFVQ 121 +G + AG + G+ V+ FKVGD V + P G+ AE +P P + F Sbjct: 59 GLGQEAAGTIEAVGAGVSGFKVGDRV--AYAGRPNGAYAEERVMPADILVKLPDAISFDT 116 Query: 122 AASIPMVGLTSWQALHERMQLRPGQKVFIPAGSGGIGTFAIQLAKHLGAT-VGTTTSSVN 180 AA++ + G+T L + ++PG + A +GGIG A Q A+ LG +GT +S Sbjct: 117 AAAMMLQGMTVQYLLRQTYAVQPGDTILFHAAAGGIGLIACQWARALGVNLIGTVSSDEK 176 Query: 181 VDLVRSLGADVVVDYKKQH-VEQVLQ-----GYDAVLGTIRGDALEKSLAILQPGSKVVS 234 L GA V++YK ++ VE+V + G V +I D SL L+P +VS Sbjct: 177 AALALQHGAAHVINYKTENFVERVKELTGGRGVQVVYDSIGKDTFLGSLDCLRPRGMMVS 236 Query: 235 L 235 Sbjct: 237 F 237 >gi|300310417|ref|YP_003774509.1| zinc-containing alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 333 Score = 79.0 bits (193), Expect = 6e-16, Method: Compositional matrix adjust. Identities = 89/331 (26%), Positives = 149/331 (45%), Gaps = 48/331 (14%) Query: 23 PAINPDEILVQVLAAGLNPIDYMIPKGMFKPIMPLQLPHTVGSDLAGIVVETGSRVTRFK 82 P ++LV+V A +NP+D I + +G D++G+V G VT F Sbjct: 27 PTPGEHDLLVEVKAISVNPVDTKIRASAKEA-------RVLGWDVSGVVRAVGPAVTLFA 79 Query: 83 VGDAVF-ASIFDLPGGSLAEFASVPEYAAALKPANLDFVQAASIPMVGLTSWQALHERMQ 141 GD VF A PG + +E V E KP++L AA+IP+ +T+W+ L +R+ Sbjct: 80 PGDEVFYAGSITRPGAN-SELHVVDERIVGRKPSSLGHADAAAIPLTAITAWELLFDRVA 138 Query: 142 LRP----GQKVFIPAGSGGIGTFAIQLAKHLG--ATVGTTTSSVNVDLVRSLGADVVVDY 195 + GQ + I +GG+G+ QLA+ L +GT + V LGA V+D+ Sbjct: 139 VAEGGGQGQHLLIIGAAGGVGSMLTQLARKLTQLTVIGTASRPETAHWVGELGAHHVIDH 198 Query: 196 K---KQHVEQV-LQGYDAVLGTIRGDA-LEKSLAILQPGSKVVSLIGPP---DAAFARAR 247 K +E++ + V+ D + + +L P + ++LI P DA + + Sbjct: 199 SQPLKPQLEKLGIPEVGIVISLTHTDQHYAQIIDVLAPQGQ-LALIDDPASLDAMPLKRK 257 Query: 248 GMNFFMKIVFGLLSAKIIRRAKKHDASYSFLFLRPDG-RQ---LAQIGDLLATGGIRPVI 303 ++ +++F +F PD RQ L ++ LL G +R Sbjct: 258 SISLHWELMF-----------------TRSMFQTPDMIRQHELLQRVSQLLDQGVLRTTT 300 Query: 304 DKVFA-FDQAKDGLAY--LEQGRAKGKVVVQ 331 + + D A A+ LE G+AKGK+V++ Sbjct: 301 GEHYGRIDAANLRRAHGLLESGKAKGKIVLE 331 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.139 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,090,607 Number of extensions: 48261 Number of successful extensions: 212 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 203 Number of HSP's successfully gapped: 6 Length of query: 334 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 248 Effective length of database: 1,212,660 Effective search space: 300739680 Effective search space used: 300739680 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)