BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0228.0002.p (197 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 191 3e-50 gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde deh... 148 3e-37 gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 146 1e-36 gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrog... 130 7e-32 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 121 3e-29 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 191 bits (485), Expect = 3e-50, Method: Compositional matrix adjust. Identities = 97/187 (51%), Positives = 127/187 (67%) Query: 1 VPERRLEEVKMLVRKAVAAIKVGHPGDEDTMLGPLVTQKQYERVQSYIRLGIEEGAELLT 60 VP L V L +A +KVG P D T GPL + Q+ RVQ I G+++GA LL Sbjct: 298 VPRAALARVHELALATLAQMKVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLA 357 Query: 61 GGEGHPEGMDHGYFVKPTVFVNVTNDMRIAREEIFGPVLSILTYRTEEEAIDIANDTVYG 120 GG G P+G+ GY+ +PT+F V DM IA+EEIFGPVL+IL Y T EEAI IANDTVYG Sbjct: 358 GGPGRPDGLAQGYYARPTIFSEVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYG 417 Query: 121 LHGYVSGADRERANRVASQIVAGRVFINGLYDEPRAPFGGFKQSGIGREFGPYGLEAYVE 180 L +V G D+++ VA++I +G+V +N +P+APFGG+KQSG GRE+G G+E Y+E Sbjct: 418 LGAHVQGRDKDQVRAVAARIQSGQVHLNYPAWDPQAPFGGYKQSGNGREYGVEGMEEYME 477 Query: 181 PKAIIGY 187 KAI+GY Sbjct: 478 VKAILGY 484 >gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 148 bits (374), Expect = 3e-37, Method: Compositional matrix adjust. Identities = 86/174 (49%), Positives = 115/174 (66%), Gaps = 4/174 (2%) Query: 15 KAVAAIKVGHPGDEDTMLGPLVTQKQYERVQSYIRLGIEEGAELLTGGEGHPEGMD--HG 72 K VAAIK G+P D+ TM+G +Q+Q E++ SYI +G +EGA++L GG G D G Sbjct: 323 KRVAAIKQGNPLDKSTMIGAQASQEQLEKILSYIDIGKQEGAKVLAGGGREELGGDLASG 382 Query: 73 YFVKPTVFVNVTNDMRIAREEIFGPVLSILTYRTEEEAIDIANDTVYGLHGYVSGADRER 132 Y+VKPTVF N MRI +EEIFGPV+S+ T++ EEEA+ IANDT+YGL + D R Sbjct: 383 YYVKPTVFQG-NNKMRIFQEEIFGPVVSVTTFKDEEEALAIANDTLYGLGAGLWTRDGTR 441 Query: 133 ANRVASQIVAGRVFINGLYDEP-RAPFGGFKQSGIGREFGPYGLEAYVEPKAII 185 A R+ +I AGRV+ N + P A FGG+KQSGIGRE L+ Y + K ++ Sbjct: 442 AFRMGREIQAGRVWTNCYHLYPAHAAFGGYKQSGIGRENHKMMLDHYQQTKNLL 495 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 146 bits (368), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 77/166 (46%), Positives = 103/166 (62%), Gaps = 4/166 (2%) Query: 20 IKVGHPGDEDTMLGPLVTQKQYERVQSYIRLGIEEGAELLTGGEGHPEGMDHGYFVKPTV 79 I VG P +GP+ + Q+ERV YI+ G++EGA LL GG P D G+FV+PT+ Sbjct: 316 ITVGDPSQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGGVSRP---DKGWFVQPTI 372 Query: 80 FVNVTNDMRIAREEIFGPVLSILTYRTEEEAIDIANDTVYGLHGYVSGADRERANRVASQ 139 F + N M IAREEIFGPV +I+++ TEEEAI +AND+ YGL V + RA+RVA+ Sbjct: 373 FADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSKYGLAATVWTTNLARAHRVAAG 432 Query: 140 IVAGRVFINGLYD-EPRAPFGGFKQSGIGREFGPYGLEAYVEPKAI 184 + G V +N + P+GG K SG+GRE G G AY E K + Sbjct: 433 VKVGAVGVNCWSPLDANLPWGGIKDSGMGREGGLAGALAYTEEKTV 478 >gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 504 Score = 130 bits (327), Expect = 7e-32, Method: Compositional matrix adjust. Identities = 70/166 (42%), Positives = 99/166 (59%), Gaps = 4/166 (2%) Query: 20 IKVGHPGDEDTMLGPLVTQKQYERVQSYIRLGIEEGAELLTGGEGHPEGMDHGYFVKPTV 79 I++G P D+ T +GP+ +KQYE +Q + G+E GA L GH + GYFV+PT+ Sbjct: 326 IRLGAPLDDSTEVGPINNRKQYEHIQRMVARGLEAGATL---AAGHTRYGEEGYFVRPTL 382 Query: 80 FVNVTNDMRIAREEIFGPVLSILTYRTEEEAIDIANDTVYGLHGYVSGADRERANRVASQ 139 + +N M +AR EIFGPV + + EEEAI IAND+ +GL G V D RA+RVA+ Sbjct: 383 LAHASNAMEVARSEIFGPVAVAIPFEDEEEAIAIANDSEFGLAGAVWTRDVARAHRVAAS 442 Query: 140 IVAGRVFINGLYD-EPRAPFGGFKQSGIGREFGPYGLEAYVEPKAI 184 + AG ++N +PFGGF +SG GR G L Y + K++ Sbjct: 443 VNAGTFWVNSYKTINVASPFGGFNRSGYGRSSGMEALYDYTQTKSV 488 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 121 bits (304), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 68/166 (40%), Positives = 101/166 (60%), Gaps = 4/166 (2%) Query: 15 KAVAAIKVGHPGDEDTMLGPLVTQKQYERVQSYIRLGIEEGAELLTGGEGHPEGMDHGYF 74 +AV KVG +E GPL+ ++ ++V+ ++ + +GA +L GG+ H G H +F Sbjct: 312 EAVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGKRHALG--HSFF 369 Query: 75 VKPTVFVNVTNDMRIAREEIFGPVLSILTYRTEEEAIDIANDTVYGLHGYVSGADRERAN 134 +PTV +VT M++AREE FGP+ + ++T+EE + +ANDT +GL Y D R Sbjct: 370 -EPTVLADVTPAMQVAREETFGPMAPLFRFKTDEEVLALANDTEFGLASYFYSRDIGRIW 428 Query: 135 RVASQIVAGRVFIN-GLYDEPRAPFGGFKQSGIGREFGPYGLEAYV 179 RVA + +G V IN GL APFGG KQSG+GRE YG++ Y+ Sbjct: 429 RVAEGLESGMVGINTGLISNEVAPFGGVKQSGLGREGSHYGIDDYL 474 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.140 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 605,585 Number of extensions: 23548 Number of successful extensions: 96 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 68 Number of HSP's successfully gapped: 18 Length of query: 197 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 117 Effective length of database: 1,241,070 Effective search space: 145205190 Effective search space used: 145205190 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 128 (53.9 bits)