BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0228.0001.p (210 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 127 7e-31 gi|300312371|ref|YP_003776463.1| methylmalonate-semialdehyde deh... 92 3e-20 gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrog... 90 1e-19 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 86 2e-18 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 84 6e-18 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 127 bits (319), Expect = 7e-31, Method: Compositional matrix adjust. Identities = 71/200 (35%), Positives = 107/200 (53%), Gaps = 1/200 (0%) Query: 4 INEIYINGRFVQPHGTEVMELINPSDKTVIGAARLADEEDARQAIAAAKAAYKTWSRSSR 63 ++ YI+G++ P G+E ++ P+ + + L + D QA+ AA+ A W+ +S Sbjct: 15 LDHFYIDGKWQPPQGSERAAVVAPATEERVCEIALGNAADVDQAVQAARRAIPAWAATSP 74 Query: 64 QERAAYLQAMHDAVQRRAPELVDVMIREYGGTRRMC-SATVARSANSFLLAIELLETFDF 122 ERAA L +H + R+ + E G +A V +A +A + L + F Sbjct: 75 LERAALLGRVHALLLERSELFAQALTLEMGAPISYARTAHVPLAAEHLRVARDNLADYPF 134 Query: 123 VRQAGTARVTMEPLGVVGLITPWNANYGFICSKLATALVAGSTTVIKPSELSAMQTRLLT 182 +R GT + EP+GV LITPWN I +K+ AL AG T V+KPSELS + L Sbjct: 135 IRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSELSPLSALLFA 194 Query: 183 QCLHEAGLPPGVFNIVTGRG 202 + +HEAG+P GVFN+V G G Sbjct: 195 EIVHEAGVPAGVFNLVNGSG 214 >gi|300312371|ref|YP_003776463.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 92.0 bits (227), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 57/199 (28%), Positives = 94/199 (47%), Gaps = 5/199 (2%) Query: 7 IYINGRFVQPHGTEVMELINPSDKTVIGAARLADEEDARQAIAAAKAAYKTWSRSSRQER 66 +Y+NG VQ E +++NP+ + V+ A +E+ +A+A AK +KTW +S +R Sbjct: 11 LYLNGEKVQSTSKEWRDVLNPATQEVVARVPFATKEEVDRAVANAKETFKTWRNTSLAQR 70 Query: 67 AAYLQAMHDAVQRRAPELVDVMIREYGGTRRMCSATVARSANSF-----LLAIELLETFD 121 + ++ L +++ RE+G T V R + +++L E + Sbjct: 71 MRIMLKFQQLLRENIAPLAELITREHGKTLPDAEGEVMRGLEVVEHACSITSLQLGELAE 130 Query: 122 FVRQAGTARVTMEPLGVVGLITPWNANYGFICSKLATALVAGSTTVIKPSELSAMQTRLL 181 V +PLGV IT +N C A+ G+T V+KPSE + L Sbjct: 131 NVAGGVDVYTLYQPLGVGAGITAFNFPVMLPCFMFPIAVACGNTFVLKPSEQDPTSSLFL 190 Query: 182 TQCLHEAGLPPGVFNIVTG 200 + ++AGLPPGV N+V G Sbjct: 191 VELANQAGLPPGVLNVVHG 209 >gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 89.7 bits (221), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 63/204 (30%), Positives = 95/204 (46%), Gaps = 5/204 (2%) Query: 4 INEIYINGRFVQPHGTEVMELINPSDKTVIGAARLADEEDARQAIAAAKAAYK-TWSRSS 62 + + +I R+V P E + +++PSD A + D QA+ AA+ AY+ W R S Sbjct: 1 MKQHFIANRWVAPTSGESIPVLDPSDGQPFDAIARGNATDIDQAVRAARLAYEGAWGRLS 60 Query: 63 RQERAAYLQAMHDAVQRRAPELVDVMIREYGGTRRMC---SATVARSANSFLLAIELLET 119 ER+ L + +Q EL + R+ G + +A + R + A + L Sbjct: 61 AAERSRLLMKLAYKLQDHQEELAQLEGRDCGKPMKQARADAAAIVRYFEFYAGAADKLHG 120 Query: 120 FDFVRQAGTARVTM-EPLGVVGLITPWNANYGFICSKLATALVAGSTTVIKPSELSAMQT 178 QAG +T+ EP GV G I PWN + AL AG+T V+KP+E + + Sbjct: 121 DTIPYQAGYTVLTLREPHGVTGHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAEDACLSI 180 Query: 179 RLLTQCLHEAGLPPGVFNIVTGRG 202 + + E G P G NIVTG G Sbjct: 181 LRVAELAAEVGFPEGALNIVTGYG 204 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 85.9 bits (211), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 61/202 (30%), Positives = 99/202 (49%), Gaps = 9/202 (4%) Query: 6 EIYINGRFVQPHGTEVMELINPSDKTVIGAARLADEEDARQAIAAAKAAYKTWSRSSRQE 65 +++ING + +++INP+ + VIG AD D +A+ AA ++TW S E Sbjct: 6 QLFINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRNMSAFE 65 Query: 66 RAAYLQAMHDAVQRRAPELVDVMIREYG---GTRRMCSATVARSANSFLLAIELLETFDF 122 R+ ++ D ++ RA E+ +M E G +M + A + + F A E T+ Sbjct: 66 RSKIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWF--AEEARRTYGR 123 Query: 123 V---RQAGTAR-VTMEPLGVVGLITPWNANYGFICSKLATALVAGSTTVIKPSELSAMQT 178 + R G + V EP+G V TPWN + KL+ AL AG + ++K E + Sbjct: 124 LVPARVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASP 183 Query: 179 RLLTQCLHEAGLPPGVFNIVTG 200 L + +AG+P GV N+V G Sbjct: 184 AELIRAYADAGVPAGVINLVYG 205 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 84.3 bits (207), Expect = 6e-18, Method: Compositional matrix adjust. Identities = 59/202 (29%), Positives = 96/202 (47%), Gaps = 7/202 (3%) Query: 5 NEIYINGRFVQPHGTEVMELINPSDKTVIGAARLADEEDARQAIAAAKAAYKTWSRSSRQ 64 + ++NG++ E + + NP++ ++G L + R+AI AA AA+ W + + + Sbjct: 12 QQAFVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANAAWPAWKKKTAK 71 Query: 65 ERAAYLQAMHDAVQRRAPELVDVMIREYGGTRRMCSATVARSANSFL--LAIELLETF-D 121 ER+A L+ ++ + +L +M E G + +A SF+ A E T+ D Sbjct: 72 ERSAILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAA-SFIEWFAEEGKRTYGD 130 Query: 122 FVRQAGTAR---VTMEPLGVVGLITPWNANYGFICSKLATALVAGSTTVIKPSELSAMQT 178 + + V E +GV ITPWN I K AL AG V+KP+E + Sbjct: 131 TIPSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSA 190 Query: 179 RLLTQCLHEAGLPPGVFNIVTG 200 L AG+P GVF++VTG Sbjct: 191 LALAVLAERAGIPAGVFSVVTG 212 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.133 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 575,561 Number of extensions: 19763 Number of successful extensions: 120 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 102 Number of HSP's successfully gapped: 19 Length of query: 210 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 129 Effective length of database: 1,236,335 Effective search space: 159487215 Effective search space used: 159487215 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 128 (53.9 bits)