BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0227.0003.p (395 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310951|ref|YP_003775043.1| poly[D(-)-3-hydroxyalkanoate] d... 737 0.0 gi|300309974|ref|YP_003774066.1| poly-beta-hydroxyalkanoate depo... 349 2e-97 gi|300309607|ref|YP_003773699.1| poly(3-hydroxyalkanoate)synthet... 81 2e-16 >gi|300310951|ref|YP_003775043.1| poly[D(-)-3-hydroxyalkanoate] depolymerase [Herbaspirillum seropedicae SmR1] Length = 411 Score = 737 bits (1902), Expect = 0.0, Method: Compositional matrix adjust. Identities = 343/395 (86%), Positives = 373/395 (94%) Query: 1 MQWAETSAKLFSDPVSPLAHAPFAQRIAAGYELLYRLGKDYEKPEFQIESTEINGKTVGI 60 MQWAETSAKLFSDPVSPLAH PF+QRIAAGYELLYRL K+YEKP+F I++ ++GK+VGI Sbjct: 17 MQWAETSAKLFSDPVSPLAHTPFSQRIAAGYELLYRLSKEYEKPQFNIDAVPVDGKSVGI 76 Query: 61 VEKRSETKPFCRLIHFSKDLDSKQFAALKQPTVLLVAPLSGHHSTLLRDTVRGLLPNHDV 120 VE+ E KPFCRLIHF KDL ++Q ALKQPTVL+VAPLSGHHSTLLR+TVR LL HDV Sbjct: 77 VEEVVEEKPFCRLIHFRKDLSARQATALKQPTVLVVAPLSGHHSTLLRETVRALLQEHDV 136 Query: 121 FITDWTDARMVPLEKGAFHLHDYVYYIQDFIRTLGPDVHVISVCQPTVPVLAAIALMASA 180 +ITDWTDARMVP+E+GAFHLHDYVYY+QDFIRTLGPDVHVISVCQPTVPVLAAI+LMASA Sbjct: 137 YITDWTDARMVPVEQGAFHLHDYVYYVQDFIRTLGPDVHVISVCQPTVPVLAAISLMASA 196 Query: 181 NDPKLPKSMTMMGGPIDARKSPTAVNDLATEKPFSWFENTVIYSVPANYPGFGRKVYPGF 240 NDP LPKSMTMMGGPIDARKSPTAVNDLATEKP+SWFENTVIYSVPANYPGFGRKVYPGF Sbjct: 197 NDPMLPKSMTMMGGPIDARKSPTAVNDLATEKPYSWFENTVIYSVPANYPGFGRKVYPGF 256 Query: 241 LQHAGFVAMNPRRHAESHWDFYMHLRDGDDESAESHRKFYDEYNAVLDMPAEFYLETIKI 300 LQHAGFVAMNPRRHA+SHWDFYMHLRDGDD SAE HRKFYDEYNAVLDMPAEFYLETIK+ Sbjct: 257 LQHAGFVAMNPRRHAQSHWDFYMHLRDGDDASAEEHRKFYDEYNAVLDMPAEFYLETIKV 316 Query: 301 VFQDFDLPQGTWNIEGKLVRPQDIKSVALFTIEGELDDISGSGQTQAAQDLCSSIPKSRK 360 VFQDF+L +GTW IEG+LVRPQDIKSVALFTIEGELDDISGSGQTQAAQ+LCSSIPK+RK Sbjct: 317 VFQDFNLARGTWEIEGQLVRPQDIKSVALFTIEGELDDISGSGQTQAAQELCSSIPKARK 376 Query: 361 QHFTAPKCGHYGIFSGRRWREVICPKIGEFIKANS 395 QHFT PK GHYGIFSGRRWRE++CPKIGEFI+AN+ Sbjct: 377 QHFTVPKAGHYGIFSGRRWREIVCPKIGEFIRANA 411 >gi|300309974|ref|YP_003774066.1| poly-beta-hydroxyalkanoate depolymerase [Herbaspirillum seropedicae SmR1] Length = 408 Score = 349 bits (895), Expect = 2e-97, Method: Compositional matrix adjust. Identities = 180/371 (48%), Positives = 239/371 (64%), Gaps = 6/371 (1%) Query: 22 PFAQRIAAGYELLYRLGKDYEKPEFQIESTEINGKTVGIVEKRSETKPFCRLIHFSKDLD 81 P+ +++A+ E+ R + +P F I + E G T+ + E+ PFC L+HF KD Sbjct: 40 PYWRKMASACEVFARTQLTHARPPFGIATVEEEGCTISVHEEEIHATPFCGLLHFRKD-- 97 Query: 82 SKQFAALKQPTVLLVAPLSGHHSTLLRDTVRGLLPNHDVFITDWTDARMVPLEKGAFHLH 141 +A QP VL+VAP+SGH +TLLR TVR LL +HDV+ITDW +AR V G F L Sbjct: 98 ----SATVQPKVLVVAPMSGHFATLLRGTVRTLLRDHDVYITDWRNARDVATAHGRFGLD 153 Query: 142 DYVYYIQDFIRTLGPDVHVISVCQPTVPVLAAIALMASANDPKLPKSMTMMGGPIDARKS 201 +YV +I DF+ LGP H+++VCQPTV L A A+MA+ P P+SMT+M GPID R + Sbjct: 154 EYVSHIIDFLGVLGPGAHLLAVCQPTVAALTAAAVMAADGHPAQPRSMTLMAGPIDTRVN 213 Query: 202 PTAVNDLATEKPFSWFENTVIYSVPANYPGFGRKVYPGFLQHAGFVAMNPRRHAESHWDF 261 PTAVN LA KP +WFE +I +VPA + G GR+VYPGF+Q A F+ MN RH E+ Sbjct: 214 PTAVNALAKSKPIAWFEKNMISTVPARHAGAGRRVYPGFVQLAAFMNMNLSRHVEAFGKL 273 Query: 262 YMHLRDGDDESAESHRKFYDEYNAVLDMPAEFYLETIKIVFQDFDLPQGTWNIEGKLVRP 321 Y HL DG+ A+ + FY+EY A+ D+PAEFYLET++ VFQ+ LP G + G+ V P Sbjct: 274 YHHLVDGEHARADQIKDFYEEYFAMADLPAEFYLETVRTVFQEHALPLGKLSYAGRPVEP 333 Query: 322 QDIKSVALFTIEGELDDISGSGQTQAAQDLCSSIPKSRKQHFTAPKCGHYGIFSGRRWRE 381 + I+ ALFTIEGE DDI GQT AAQ+LCS I + H GHYG+F+GRRW Sbjct: 334 RAIRRTALFTIEGEKDDICAVGQTLAAQELCSGIRPYMRLHHVQTAVGHYGVFNGRRWDN 393 Query: 382 VICPKIGEFIK 392 I P++ +FI Sbjct: 394 EIYPRLRDFIN 404 >gi|300309607|ref|YP_003773699.1| poly(3-hydroxyalkanoate)synthetase [Herbaspirillum seropedicae SmR1] Length = 376 Score = 80.9 bits (198), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 80/310 (25%), Positives = 135/310 (43%), Gaps = 38/310 (12%) Query: 94 LLVAPLSGHHSTL--------LRDTVRGLLPNHDVFITDW--TDARMVPLEKGAFHLHDY 143 L+VAP +GH S + L +T+ G +H V + DW A M LE + +Y Sbjct: 89 LIVAPYAGHTSVIADYDQGQSLVETLLGNGVDH-VLLIDWHSASADMKDLE-----IDNY 142 Query: 144 VYYIQDFIRTLGPDVHVISVCQPTVPVLAAIALMASANDPKLPKSMTMMGGPIDARKSPT 203 + + I LG V ++ +CQ I+ M +A P+ + + G PIDA Sbjct: 143 LADLCVVIDELGGRVRLVGLCQG-----GWISAMIAARFPEKVARLVLAGSPIDAGAGEG 197 Query: 204 AVNDLATEKPFSWFENTVIYSVPANYPGFGRKVYPGFLQHAGFVAMNPRRHA-ESHWDFY 262 + + P ++E+ V G + G G+ M+P H H + Sbjct: 198 PLKQMVQRLPLRFYEDLVRS---------GGGLMRGSFMLQGWKNMHPDEHYFNEHLELL 248 Query: 263 MHLRDGDDESAESHRK-FYDEYNAVLDMPAEFYLETIKIVFQDFDLPQGTWNIEGKLVRP 321 H+ DD + + R+ F Y +D+P +YL+ I +F+D L +G + G+ + Sbjct: 249 QHI---DDPAYLAKRETFAAWYETTVDLPGRWYLQAIGHIFKDNLLARGKYVALGQQLDL 305 Query: 322 QDIKSVALFTIEGELDDISGSGQTQAAQDLCSSIPKSRKQHFTAPKCGHYGIFSGRRWRE 381 + I + L+ + GE DDI+ Q A L + P ++ P GH G+F G R Sbjct: 306 RQI-TCPLYLLAGERDDITTPEQVLNAAALVGT-PAAQITQKIVPG-GHIGLFMGSRTLA 362 Query: 382 VICPKIGEFI 391 + P+I ++ Sbjct: 363 EVWPQIATWL 372 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,308,784 Number of extensions: 55450 Number of successful extensions: 132 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 129 Number of HSP's successfully gapped: 3 Length of query: 395 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 308 Effective length of database: 1,207,925 Effective search space: 372040900 Effective search space used: 372040900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)