BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0226.0008.p (332 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosp... 272 3e-74 gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosp... 211 5e-56 gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosp... 105 5e-24 gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosp... 76 3e-15 gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase ... 64 2e-11 >gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 585 Score = 272 bits (695), Expect = 3e-74, Method: Compositional matrix adjust. Identities = 124/135 (91%), Positives = 129/135 (95%) Query: 4 DKKRTLRSAAWFGTADKNGFMYRSWMKNQGIPDHEFQGKPVIGICNTWSELTPCNAHFRK 63 DK RTLRSA WFGTADKNGFMYRSWMKNQGIPDHEFQGKPVIGICNTWSELTPCNAHFRK Sbjct: 7 DKSRTLRSAGWFGTADKNGFMYRSWMKNQGIPDHEFQGKPVIGICNTWSELTPCNAHFRK 66 Query: 64 IAEHVKRGIIEAGGFPVEFPVFSNGESNLRPTAMLTRNLASMDVEESIRGNPLDAVVLLV 123 IAEHV+RGIIEAGGFPVEFPVFSNGESNLRPTAMLTRNLASMDVEESIRGNP+DAVVLL Sbjct: 67 IAEHVRRGIIEAGGFPVEFPVFSNGESNLRPTAMLTRNLASMDVEESIRGNPIDAVVLLT 126 Query: 124 GCDKTTPEVLKPSAA 138 GCDKTTP +L +A+ Sbjct: 127 GCDKTTPALLMGAAS 141 Score = 255 bits (652), Expect = 3e-69, Method: Compositional matrix adjust. Identities = 122/142 (85%), Positives = 132/142 (92%) Query: 132 VLKPSAASPELMKHRGKAVVFEDFAHYKERINDPDLDVDASSVLVMKNCGPKGYPGMAEV 191 VLKPSAA+PELMKHRG+AVVFEDF HYKERINDPDLDVDAS VLVMKN GPKGYPGMAEV Sbjct: 397 VLKPSAATPELMKHRGRAVVFEDFNHYKERINDPDLDVDASCVLVMKNVGPKGYPGMAEV 456 Query: 192 GNMGLPPKLLAQGVKDMVRISDARMSGTAYGTVVLHVAPEAAAGGPLGIVQDGDWIEMDC 251 GNMGLPPK+LA GVKDMVRISDARMSGTAYGTV+LHVAPEAAAGGPLGIVQDGD+IE+D Sbjct: 457 GNMGLPPKVLATGVKDMVRISDARMSGTAYGTVILHVAPEAAAGGPLGIVQDGDFIELDA 516 Query: 252 SAGRLHLDISDEEMAQRQTARS 273 AG+L LDIS+EEM +R AR+ Sbjct: 517 YAGKLQLDISEEEMKRRLEARA 538 >gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 578 Score = 211 bits (538), Expect = 5e-56, Method: Compositional matrix adjust. Identities = 119/228 (52%), Positives = 151/228 (66%), Gaps = 18/228 (7%) Query: 56 PCNAHFRKIAEHVKRGIIEAGGFPVEFPVFSNGESNLRPTAMLTRNLASM-----DV--- 107 PC + + + AGG P V ES + TA LT N ++ D Sbjct: 306 PCLLDLQPSGKFLMEDFYYAGGLPA---VIRQLESVIDKTA-LTANGKTLWENCQDAPNW 361 Query: 108 -EESIRG--NPLD---AVVLLVGCDKTTPEVLKPSAASPELMKHRGKAVVFEDFAHYKER 161 EE IR P + +L G V+KPSAA+P L+KHRG+AVVFE+ +R Sbjct: 362 NEEVIRSFDKPFKEAAGIAILKGNLAPDGAVIKPSAATPALLKHRGRAVVFENSDDLHKR 421 Query: 162 INDPDLDVDASSVLVMKNCGPKGYPGMAEVGNMGLPPKLLAQGVKDMVRISDARMSGTAY 221 I+D +LDVD + VLV+KNCGPKGYPGMAE GNM LPPK+L +G+ DMVR+SDARMSGTAY Sbjct: 422 IDDENLDVDETCVLVLKNCGPKGYPGMAEAGNMPLPPKILRKGITDMVRVSDARMSGTAY 481 Query: 222 GTVVLHVAPEAAAGGPLGIVQDGDWIEMDCSAGRLHLDISDEEMAQRQ 269 GTVVLHV+PEAAAGGPL +VQ+GD+IE+D A +LHLD+SDEE+A+R+ Sbjct: 482 GTVVLHVSPEAAAGGPLAVVQNGDFIELDVEARKLHLDVSDEELARRR 529 Score = 211 bits (538), Expect = 5e-56, Method: Compositional matrix adjust. Identities = 100/139 (71%), Positives = 116/139 (83%), Gaps = 1/139 (0%) Query: 1 MTID-KKRTLRSAAWFGTADKNGFMYRSWMKNQGIPDHEFQGKPVIGICNTWSELTPCNA 59 MT D KK T RS AWFG D++GF+YRSW+KN+GIP +F G+PVIGICNT+SELTPCN+ Sbjct: 1 MTDDTKKSTRRSQAWFGRQDRDGFIYRSWVKNRGIPHDQFDGRPVIGICNTYSELTPCNS 60 Query: 60 HFRKIAEHVKRGIIEAGGFPVEFPVFSNGESNLRPTAMLTRNLASMDVEESIRGNPLDAV 119 HFR +AE VK GI EAGGFP+EFPV S GE+ LRPTAML RNLASMDVEESIR NPLD V Sbjct: 61 HFRALAEQVKIGIWEAGGFPLEFPVMSLGETLLRPTAMLYRNLASMDVEESIRANPLDGV 120 Query: 120 VLLVGCDKTTPEVLKPSAA 138 VLL+GCDKTTP +L +A+ Sbjct: 121 VLLMGCDKTTPALLMGAAS 139 >gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 105 bits (262), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 56/134 (41%), Positives = 79/134 (58%), Gaps = 3/134 (2%) Query: 7 RTLRSAAWFGTADKNGF--MYRSWMKNQGIPDHEFQ-GKPVIGICNTWSELTPCNAHFRK 63 R RS WF D +Y N GI E + G+P+IGI + S+++PCN + Sbjct: 9 RRFRSQDWFDNPDHIDMTALYLERFMNYGITAEELRSGRPIIGIAQSGSDISPCNRIHLE 68 Query: 64 IAEHVKRGIIEAGGFPVEFPVFSNGESNLRPTAMLTRNLASMDVEESIRGNPLDAVVLLV 123 +A+ V+ GI +AGG P+EFP+ E+ RPTA + RNLA + + E + G P+DAVVL Sbjct: 69 LAKRVRDGIRDAGGIPMEFPLHPIFENCRRPTAAIDRNLAYLGLVEILHGYPIDAVVLTT 128 Query: 124 GCDKTTPEVLKPSA 137 GCDKTTP + +A Sbjct: 129 GCDKTTPSQIMAAA 142 Score = 103 bits (256), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 49/123 (39%), Positives = 80/123 (65%) Query: 148 KAVVFEDFAHYKERINDPDLDVDASSVLVMKNCGPKGYPGMAEVGNMGLPPKLLAQGVKD 207 +AVVF+ Y RINDP L +D +++L ++ GP G+PG AEV NM P L+ +GV Sbjct: 424 RAVVFDGSDDYHARINDPALKIDENTLLAIRGAGPVGWPGSAEVVNMQPPDALIKRGVST 483 Query: 208 MVRISDARMSGTAYGTVVLHVAPEAAAGGPLGIVQDGDWIEMDCSAGRLHLDISDEEMAQ 267 + + D R SGT+ +L+ +PE+A GG L ++DGD + +D + G ++ +S+EE+A+ Sbjct: 484 LPTLGDGRQSGTSDSPSILNASPESAVGGGLAYLRDGDRVRIDLNTGECNMLVSEEELAR 543 Query: 268 RQT 270 R++ Sbjct: 544 RKS 546 >gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 620 Score = 76.3 bits (186), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 50/142 (35%), Positives = 80/142 (56%), Gaps = 7/142 (4%) Query: 132 VLKPSAASPELMKHRGKAVVFEDFAHYKERINDPDLDVDASSVLVMKNCGPKGYPGMAEV 191 ++K + ++K G A VFE E I V A V++++ GPKG PGM E Sbjct: 439 IVKTAGVDESILKFSGTARVFESQDAAVEGILGDK--VKAGDVVIVRYEGPKGGPGMQE- 495 Query: 192 GNMGLPPKLL-AQGV-KDMVRISDARMSGTAYGTVVLHVAPEAAAGGPLGIVQDGDWIEM 249 M P + ++G+ K +D R SG + G V+ H +PEAA GG +G+V++GD I++ Sbjct: 496 --MLYPTSYIKSKGLGKACALFTDGRFSGGSSGLVIGHASPEAAEGGAIGLVEEGDMIDI 553 Query: 250 DCSAGRLHLDISDEEMAQRQTA 271 D ++L IS +++A+R+TA Sbjct: 554 DIPNRTINLRISSDDLAKRRTA 575 Score = 58.9 bits (141), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 36/126 (28%), Positives = 62/126 (49%), Gaps = 6/126 (4%) Query: 13 AWFGTADKNGFMYRSWMKNQGIPDHEFQGKPVIGICNTWSELTPCNAHFRKIAEHVKRGI 72 ++ T +N R+ + G+ D +F KP+I + N++++ P + H + + + V R I Sbjct: 6 SYTTTQGRNMAGARALWRATGMKDGDFT-KPIIAVVNSFTQFVPGHVHLKDLGQLVAREI 64 Query: 73 IEAGGFPVEFPVFSNGESNLRPTAMLTRNLASMD-----VEESIRGNPLDAVVLLVGCDK 127 AGG EF + +S A + +L S D VE + + DA+V + CDK Sbjct: 65 EAAGGVAKEFNTIAVDDSGTHGDARMLYSLPSRDLIADSVEYMVNAHCADAMVCISNCDK 124 Query: 128 TTPEVL 133 TP +L Sbjct: 125 ITPGML 130 >gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 623 Score = 63.5 bits (153), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 58/173 (33%), Positives = 77/173 (44%), Gaps = 12/173 (6%) Query: 132 VLKPSAASPELMKHRGKAVVFEDFAHYKERINDPDLDVDASSVLVMKNCGPKGYPGMAEV 191 V+K SA PE AVVF + LD D +V+ + GP+ GM E+ Sbjct: 440 VIKISAVKPEHRVVEAPAVVFHSQEAFMAAFKAGQLDRDFVAVITFQ--GPRAN-GMPEL 496 Query: 192 GNMGLPPKLLAQGVKDMVRISDARMSGTAYGTV--VLHVAPEAAAGGPLGIVQDGDWIEM 249 + +L + + ++D RMSG A G V +HV PE GGPLG V+DGD I + Sbjct: 497 HALTPALGILQDKGRHVALVTDGRMSG-ASGKVPAAIHVTPEVLGGGPLGRVRDGDIIRL 555 Query: 250 DCSAGRLHLDISDEEMAQRQTARSCKACRASARKTARSAGRNLSSAARTTCRT 302 D AG L + E A ARS + S + GR L S R T Sbjct: 556 DAEAGTLEAKVDATEWA----ARSQDSSDLSGNQ--HGMGRELFSLFRNAVGT 602 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.131 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,013,190 Number of extensions: 42664 Number of successful extensions: 104 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 101 Number of HSP's successfully gapped: 9 Length of query: 332 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 246 Effective length of database: 1,212,660 Effective search space: 298314360 Effective search space used: 298314360 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)