BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0226.0005.p (369 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310376|ref|YP_003774468.1| outer membrane porin [Herbaspir... 452 e-128 gi|300313813|ref|YP_003777905.1| transmembrane outer membrane po... 137 1e-33 gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) ... 113 3e-26 gi|300310614|ref|YP_003774706.1| outer membrane porin [Herbaspir... 111 7e-26 gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) ... 106 4e-24 >gi|300310376|ref|YP_003774468.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 355 Score = 452 bits (1162), Expect = e-128, Method: Compositional matrix adjust. Identities = 247/368 (67%), Positives = 286/368 (77%), Gaps = 31/368 (8%) Query: 20 FGACLASAQAE-DSVTIYGRVVAGVDVQSNVHQANGA--TGTQVRAASNQWGTSMIGFKG 76 GA A AQA+ SVTIYGRVVAGVD+ +NV + +G+ TGT++RAASNQWGTSMIG KG Sbjct: 1 MGAFSAQAQAQTSSVTIYGRVVAGVDIMTNVPKTDGSGTTGTRIRAASNQWGTSMIGVKG 60 Query: 77 KEDLGGGLNSFFLLESGFGATKGATNGPE-----LFNRRSYVGLNGS-AGSVKIGKNLFI 130 EDLGGG N+FFLLESGFG+T G NG + LFNRRSYVGL+ + AGS+K+GKNLFI Sbjct: 61 TEDLGGGTNAFFLLESGFGSTTGGLNGTDINGTSLFNRRSYVGLSSANAGSLKLGKNLFI 120 Query: 131 DNDVWFLDPTGQQWIGTANLVHGRNWGGANNIIEYQTPTWGGFNATFQTSLGEKAGSTTK 190 +NDVWFLDPTGQQWIGTANLV GRNW GA+N+IEY TPT+GGF AT QTSLGE AGS +K Sbjct: 121 NNDVWFLDPTGQQWIGTANLVRGRNWPGASNVIEYTTPTFGGFGATVQTSLGEAAGSFSK 180 Query: 191 G-------RKDGVSLVYENQGLELRAIYDLVRDANGKYGSIKTNQDGTTTAGNVYDSSKE 243 G RKDG+SLVY+N GLELR IYD++RDANG+Y +VYD+SKE Sbjct: 181 GGTSTKYGRKDGLSLVYQNNGLELRGIYDVIRDANGRYT-------------DVYDTSKE 227 Query: 244 LILGGTYTIDKLKMYLGYENLKAPD--VAPGSTDKVNHYWIGANYQLSPALTLIGSVFHV 301 LILGGTY IDKLKM+LGYENL+APD A G D+VNHYW+GANY ++P L+GSVFHV Sbjct: 228 LILGGTYKIDKLKMFLGYENLRAPDANTASGVPDRVNHYWLGANYDVTPQFQLVGSVFHV 287 Query: 302 NANNNGGSANLFMAGLNYNLSKRTLLYVGAGTVRNSGAGTFSLEADEAGAPLAGQSQTGG 361 NANN GSANLFMAG+NY LSKRTLLYVG GT+RN +SLEAD + G +QTGG Sbjct: 288 NANNGNGSANLFMAGVNYFLSKRTLLYVGGGTMRNGNNANYSLEADSSTGGGKGTNQTGG 347 Query: 362 YFGIAHSF 369 YFGIAHSF Sbjct: 348 YFGIAHSF 355 >gi|300313813|ref|YP_003777905.1| transmembrane outer membrane porin signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 354 Score = 137 bits (345), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 128/361 (35%), Positives = 171/361 (47%), Gaps = 41/361 (11%) Query: 26 SAQAEDSVTIYGRVVAGVDVQSNVHQANGATGTQVRAASNQWGTSMIGFKGKEDLGGGLN 85 S QA+ SVTIYG V GV+ +N NGA G+ R S TS IGF+G EDLGGGL Sbjct: 18 SVQAQSSVTIYGLVDTGVEYLNNAG-PNGA-GSVTRMTSGATNTSRIGFRGTEDLGGGLK 75 Query: 86 SFFLLESGFGATKGATNGP--ELFNRRSYVGLNGSAGSVKIGKNLFIDNDVWFL---DPT 140 + F LE+GF G+ +G +LFNR+S VGL G G V G++ D FL DP Sbjct: 76 ALFQLENGFKLDNGSFDGDANQLFNRQSTVGLEGGFGRVVAGRSYSTTYD--FLGPFDPM 133 Query: 141 G----QQWIGTANLVHGRNWG---GANNIIEYQTPTWGGFNATFQTSLGEKAGSTTKGRK 193 G W TA R G G +N+++YQ + G GE AG T G K Sbjct: 134 GYSANYSWATTAGATGNRKDGMPTGVSNLVKYQGE-FSGVKIGAMYGFGEVAGETRNGAK 192 Query: 194 DGVSLVYENQGLELRAIYDLVRDANGKYGSIKTNQDGTTTAGNV-YDSSKELILGGTYTI 252 GV L + + A YD ++ + TA N YD + L Y Sbjct: 193 YGVGLGWGTGPFSVVATYD---------------RNNSATATNTPYDRATSAHLAAGYQP 237 Query: 253 -DKLKMYLGYENLKAPDVAPGSTD-KVNHYWIGANYQLSPALTLIGSVFHVNANNNGGSA 310 + K LGY K +A G+ D + + +W GA Y+ +PA+TLIG++++ N N A Sbjct: 238 SEAWKFNLGYRYYK-KSLATGAADLRSDFFWGGAAYKFTPAMTLIGAIYYQNIKNVANDA 296 Query: 311 NLFMAGLN--YNLSKRTLLYVGAGTVRNSGAGTFSLEADEAGAPLAGQSQTGGYFGIAHS 368 + M L Y +SKRT LY+ + L D+AG G SQ G G+ H Sbjct: 297 DPIMYSLRLKYAMSKRTDLYLSTAYAKAKNGQLVGLSRDDAG---YGSSQFGMTLGMQHR 353 Query: 369 F 369 F Sbjct: 354 F 354 >gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 352 Score = 113 bits (282), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 112/374 (29%), Positives = 167/374 (44%), Gaps = 40/374 (10%) Query: 12 KALGTASLFGACLASAQAEDSVTIYGRVVAGVDVQSNVHQANGATGTQVRAASNQWGTSM 71 K L A++ +A A+ SV+IYG + G+ S+ ++ G +R S S Sbjct: 3 KTLLVAAMLAGGATTAHAQSSVSIYGLIDLGLTYYSSAPKSGGGGARLLRMDSGIAQGSR 62 Query: 72 IGFKGKEDLGGGLNSFFLLESGFGATKGAT-NGPELFNRRSYVGLNGSA-GSVKIGKNL- 128 IGFKG EDLGGG+N+FF LE+GFGA G+ G LF R+S+VGL + GS+ +G+ Sbjct: 63 IGFKGTEDLGGGMNAFFTLETGFGADDGSLGQGGLLFGRQSFVGLGSTQWGSLSVGRQYD 122 Query: 129 FIDN-----DVWFLDPTGQ-QWIGTANLVHG-----RNWGG--ANNIIEYQTPTWGGFNA 175 F+ N + L P G W A+ HG + G NN I+Y + +GGF+ Sbjct: 123 FMANIGGQYAMGALSPAGSFGWGLHADAAHGTALNNHIYAGDRTNNSIKYNSAKFGGFSF 182 Query: 176 TFQTSLGEKAGSTTKGRKDGVSLVYENQGLELRAIYDLVRDANGKYGSIKTNQDGTTTAG 235 LGE AG+ GR + Y+N Y ++D+ G + Sbjct: 183 GAMVGLGETAGNNNAGRTVSATGQYDNGPFSGALAYTDIKDSTGSF-------------- 228 Query: 236 NVYDSSKELILGGTYTIDKLKMYLGYENLKAPDVAPGSTDKVNHYWIGANYQLSPALTLI 295 S++ G Y I + +K + + K Y +G Y L+P L Sbjct: 229 ----STRIYGGGARYQIGAFTPFGVVTQVKNTNTSA----KATTYELGTVYALTPLWDLA 280 Query: 296 GSVFHVNANNNGGSANLFMAGLNYNLSKRTLLYVGAGTVRNSGAGTFSLEADEAGAPLAG 355 + N G+A +A ++Y +SKRT LYVGA R+ G + + G A Sbjct: 281 AAYQFQKRNQGVGNAQALVATVDYKISKRTDLYVGAVYDRDKGYNAYPVFG--GGIQSAT 338 Query: 356 QSQTGGYFGIAHSF 369 +QT G+ H F Sbjct: 339 GNQTALRLGMRHRF 352 >gi|300310614|ref|YP_003774706.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 369 Score = 111 bits (278), Expect = 7e-26, Method: Compositional matrix adjust. Identities = 118/389 (30%), Positives = 176/389 (45%), Gaps = 51/389 (13%) Query: 8 KTILKALGTASLFGACLASAQAEDSVTIYGRVVAGVDVQSNVHQANGATGTQVRAASNQW 67 K I+ AL T S A L+ AQ SVT+YG + GV+ SNV +R + Sbjct: 5 KAIIAALLTMS---ATLSQAQ---SVTLYGIIDTGVEYVSNVSNVGAGQQGLLRVPNLTA 58 Query: 68 GT-SMIGFKGKEDLGGGLNSFFLLESGFGATKGATN-GPELFNRRSYVGLNGSAGSVKIG 125 T S IG +G EDLGGGL S F+LESGF GA N G +F R +YVGL+ G V G Sbjct: 59 TTPSRIGLRGSEDLGGGLRSVFMLESGFAPDSGALNQGGRMFGRIAYVGLSDHWGQVSFG 118 Query: 126 KNLF------IDNDVWFLDPTGQQWIGTANLVHGRNWGGANNIIEYQ----------TPT 169 + +D+D+ G G L + R A+N I Y+ T + Sbjct: 119 RQATMLLWATLDSDI-----LGANVYGANALDNYRPNARADNSIAYRGSFGAFTLGGTYS 173 Query: 170 WG------GFNATFQTSLGEKAGSTTKGRKDGVSLVYENQGLELRAIYDLVRDANGKYGS 223 +G G N GE+AG + R+ + + Y+ YD +R G Sbjct: 174 FGRDTVNAGQNPAGTNCAGEQAGDSLACREWSLMVKYDTAAWGASLAYDTLRGGKGAAAG 233 Query: 224 IKTNQDGTTTAGNVYDSSKELILGGTYTIDKLKMYLGYENLKAPDVAPGSTDKVNHYWIG 283 + ++Q S ++LG D+LK+ +G +L+ + S ++ Y+ G Sbjct: 234 LSSSQL----------SDSRMLLGAYIKADRLKVGIG-GSLRHNQGSALSKSQL--YYAG 280 Query: 284 ANYQLSPALTLIGSVFHVNANNNGGSANLFMAGLNYNLSKRTLLYVGAGTVRNSGAGTFS 343 +Y L+P L L G +++++ +++ A L Y+LS RT LY+ G + N G S Sbjct: 281 LSYDLTPTLNLAGQLYYLHYDDSANKAWLGALRATYSLSARTALYLTGGFIDNRGQSNLS 340 Query: 344 LEADEA---GAPLAGQSQTGGYFGIAHSF 369 + A + P G +Q G GI HSF Sbjct: 341 VSAGGSVGTANPAPGGNQFGMMAGIRHSF 369 >gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 390 Score = 106 bits (264), Expect = 4e-24, Method: Compositional matrix adjust. Identities = 118/336 (35%), Positives = 151/336 (44%), Gaps = 44/336 (13%) Query: 32 SVTIYGRVVAGVDVQSNVHQANGATGTQVRAASNQWGTSMIGFKGKEDLGGGLNSFFLLE 91 SVTIYG V GV QS V G TG++ S S IGFKG EDLGGGL++ F LE Sbjct: 23 SVTIYGIVDTGVVYQSKVATPTGGTGSKFSLNSGVIQGSRIGFKGTEDLGGGLSAVFNLE 82 Query: 92 SGFGATKGATNGPE----LFNRRSYVGLNGSAGSVKIGKNL-FIDNDVWFLDPTGQQ--- 143 +GF GA + + LF R+S VGL G+ GSV +G+ F D + T Q Sbjct: 83 AGFSNDTGALDDSKTSNTLFRRKSVVGLAGNFGSVLLGRQTDFADTISSY---TSVQDFG 139 Query: 144 -WIGTANLVHGRNWGG-ANNIIEYQTPTWGGFNATFQTSLGEKAGSTTKGRKDGVSLVYE 201 W G A R G NN I Y T GF GE AG T+ G+ GV Y+ Sbjct: 140 GWTGAAGHNLDRLEGTRTNNSISYTTANLNGFTGNLMYGFGETAGQTSAGQAFGVGGKYD 199 Query: 202 NQGLELRAIYDLVR------DANGKYGSIKTNQDGTTTAGNVYDSSKELILGGTYTIDKL 255 N L L Y + DA+ + TNQ T N + K L + +Y Sbjct: 200 NGPLGLGINYYQSKAGSTPADAS-LLAATGTNQF-TNGTNNGSTAQKVLNVAASYQFGPA 257 Query: 256 KMYLGYE----------NLKAPDVAP---------GSTDKVNHYWIGANYQLSPALTLIG 296 ++Y + NL A V +++K + Y IG Y LSP+L L+ Sbjct: 258 RVYGNWSRVKQDLNTQANLTAAGVGSLATISNRTLAASNKADIYEIGTAYSLSPSLKLLA 317 Query: 297 SVFHVNANNNG--GSANLFMAGL--NYNLSKRTLLY 328 +V H A +G G A L L +Y LSKRT LY Sbjct: 318 AVDHTRAQFDGVSGKAKLTQVSLGTDYWLSKRTDLY 353 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.132 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,234,189 Number of extensions: 56505 Number of successful extensions: 201 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 150 Number of HSP's successfully gapped: 15 Length of query: 369 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 282 Effective length of database: 1,207,925 Effective search space: 340634850 Effective search space used: 340634850 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 131 (55.1 bits)