BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0226.0003.p (228 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312873|ref|YP_003776965.1| hypothetical protein Hsero_3578... 348 2e-97 gi|300310596|ref|YP_003774688.1| 2-keto-4-pentenoate hydratase/2... 117 1e-27 >gi|300312873|ref|YP_003776965.1| hypothetical protein Hsero_3578 [Herbaspirillum seropedicae SmR1] Length = 227 Score = 348 bits (893), Expect = 2e-97, Method: Compositional matrix adjust. Identities = 162/201 (80%), Positives = 181/201 (90%) Query: 1 MSYAIPVPAVTTVPVVGGDPFPVRRIYCVGRNYAAHAREMGHDPNREAPFFFMKPTDAIV 60 MS+AIP PA TV VVGG FPVRRIYCVGRNYAAHAREMGHDP+RE PFFFMKP DA+V Sbjct: 1 MSFAIPAPAQATVAVVGGAAFPVRRIYCVGRNYAAHAREMGHDPDREPPFFFMKPADAVV 60 Query: 61 PTGSAVPYPSKTSDYQHEIELVVALGKGGRDVPADQALDLVFGYAVGLDMTRRDLQGEAK 120 PT S VPYP TSDY HEIELVVAL KGGR++P +QAL+LVFGYAVGLDMTRRD+Q +AK Sbjct: 61 PTDSVVPYPVATSDYHHEIELVVALAKGGRNIPVEQALELVFGYAVGLDMTRRDIQSQAK 120 Query: 121 KLARPWDMGKGFDHSAPCAPLLPVSKIGHPEQGAIWLKVNGEVRQQGDLSDLIWNVAETI 180 KL RPWDM KGFD SAPCAP++PV++ GHP+QGA+WLKVNGEVRQQGDL+DLIW+VAETI Sbjct: 121 KLGRPWDMAKGFDQSAPCAPIVPVAQAGHPDQGAVWLKVNGEVRQQGDLADLIWSVAETI 180 Query: 181 SYLSGLVELFPGDLIYTGTPQ 201 SYLSGLVELFPGDLI+TGTP+ Sbjct: 181 SYLSGLVELFPGDLIFTGTPE 201 >gi|300310596|ref|YP_003774688.1| 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase [Herbaspirillum seropedicae SmR1] Length = 281 Score = 117 bits (292), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 74/198 (37%), Positives = 105/198 (53%), Gaps = 11/198 (5%) Query: 10 VTTVPVVGGDPF------PVRRIYCVGRNYAAHAREMGHDPNREAPFFFMKPTDAIVPTG 63 + T+PVV G+P + + C+G NYA HA E + P P F K T A+V Sbjct: 51 LNTLPVVEGNPRIGACVGNIGKFICIGLNYADHAAE-SNLPIPAEPVVFNKWTSAVVGPN 109 Query: 64 SAVPYPSKTSDYQHEIELVVALGKGGRDVPADQALDLVFGYAVGLDMTRRDLQGEAKKLA 123 V P + E+EL V +GKGG + A+ V GY V D++ R+ Q E Sbjct: 110 DNVKIPRGSKKTDWEVELGVIIGKGGSYIDEKDAMSHVAGYCVVNDVSEREYQIER---G 166 Query: 124 RPWDMGKGFDHSAPCAP-LLPVSKIGHPEQGAIWLKVNGEVRQQGDLSDLIWNVAETISY 182 WD GKG D P P L+ ++ P++ +WL+V+G+ Q G+ S +I+NVA +SY Sbjct: 167 GTWDKGKGCDTFGPIGPWLVTRDEVADPQKLGMWLEVDGKRYQNGNTSTMIFNVAHIVSY 226 Query: 183 LSGLVELFPGDLIYTGTP 200 LS + L PGD+I TGTP Sbjct: 227 LSRFMSLQPGDVISTGTP 244 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.139 0.444 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 828,030 Number of extensions: 38933 Number of successful extensions: 84 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 82 Number of HSP's successfully gapped: 2 Length of query: 228 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 146 Effective length of database: 1,231,600 Effective search space: 179813600 Effective search space used: 179813600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)