BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0226.0001.p (243 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312872|ref|YP_003776964.1| ATP-dependent RNA helicase [Her... 177 1e-45 gi|300311049|ref|YP_003775141.1| ATP-dependent RNA helicase [Her... 98 5e-22 gi|300312721|ref|YP_003776813.1| ATP-dependent RNA helicase [Her... 80 1e-16 >gi|300312872|ref|YP_003776964.1| ATP-dependent RNA helicase [Herbaspirillum seropedicae SmR1] Length = 487 Score = 177 bits (448), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 86/103 (83%), Positives = 89/103 (86%) Query: 16 VRFEDFGLSPDILKALTAQGYIHPTPIQEQAIPIVLQGRDVMGAAQTGTGKTAGFSLPII 75 VRFEDFGLSPDILKAL QGY+HPTPIQ QAIP+VLQGRDVMGAAQTGTGKTAGFSLPII Sbjct: 16 VRFEDFGLSPDILKALAEQGYVHPTPIQAQAIPVVLQGRDVMGAAQTGTGKTAGFSLPII 75 Query: 76 QLLLAHASTSMSPARHPVRALILTPTRELADQVAXEKLIKIKF 118 Q LL HAS S SPARHPVRALILTPTRELADQVA +F Sbjct: 76 QRLLQHASHSASPARHPVRALILTPTRELADQVADNVAAYCRF 118 Score = 102 bits (253), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 60/105 (57%), Positives = 65/105 (61%), Gaps = 27/105 (25%) Query: 111 EKLIKIKFVRAELAGFVPRQRSERSDRGGERGERPERSGRGERTERGDRGDRGERSSSAA 170 EKLIK KF RAEL GF PR R ERSD R ER ERS RGER++RGDR DRGERS S+ Sbjct: 378 EKLIKKKFERAELTGFAPRARHERSD----RAERGERSERGERSDRGDRSDRGERSRSS- 432 Query: 171 SGSSDGERSSVRRDTQSYGRSGG---VRREKVDPWFLKPYEPTAA 212 R GG +RREKVDPWFLKPYEP+A Sbjct: 433 -------------------REGGAAPMRREKVDPWFLKPYEPSAV 458 >gi|300311049|ref|YP_003775141.1| ATP-dependent RNA helicase [Herbaspirillum seropedicae SmR1] Length = 520 Score = 98.2 bits (243), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 51/91 (56%), Positives = 65/91 (71%), Gaps = 1/91 (1%) Query: 18 FEDFGLSPDILKALTAQGYIHPTPIQEQAIPIVLQGRDVMGAAQTGTGKTAGFSLPIIQL 77 F GLS +I++A++ QGY PTPIQ QA+P VL G D++ AQTGTGKTAGF+LP++Q Sbjct: 3 FSALGLSDEIVRAVSEQGYTSPTPIQAQAVPAVLSGGDLLAGAQTGTGKTAGFTLPLLQR 62 Query: 78 LLAHASTSMSPARHPVRALILTPTRELADQV 108 L A ++ P+RALIL PTRELA QV Sbjct: 63 LSAMPRQKIN-GHVPIRALILAPTRELAAQV 92 >gi|300312721|ref|YP_003776813.1| ATP-dependent RNA helicase [Herbaspirillum seropedicae SmR1] Length = 510 Score = 80.1 bits (196), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 48/110 (43%), Positives = 64/110 (58%), Gaps = 19/110 (17%) Query: 18 FEDFGLSPDILKALTAQGYIHPTPIQEQAIPIVLQGRDVMGAAQTGTGKTAGFSLPIIQL 77 FE GL+P I+KALT GY PTP+QE+AIP + G+D++ ++QTG+GKTA F LP + Sbjct: 3 FEALGLNPSIIKALTEAGYTSPTPVQEKAIPAAISGQDLLVSSQTGSGKTAAFMLPSLHQ 62 Query: 78 LLA-----HASTSMSPARHPVRA--------------LILTPTRELADQV 108 L A+ + + R RA L+LTPTRELA QV Sbjct: 63 LADLPPAPQAARTPNQERQASRARGERPRYQPAQPKMLVLTPTRELALQV 112 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.313 0.131 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 540,921 Number of extensions: 16480 Number of successful extensions: 50 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 47 Number of HSP's successfully gapped: 4 Length of query: 243 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 160 Effective length of database: 1,226,865 Effective search space: 196298400 Effective search space used: 196298400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 129 (54.3 bits)