BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0225.0058.p (447 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309753|ref|YP_003773845.1| HipA domain-containing protein ... 410 e-116 gi|300313385|ref|YP_003777477.1| HipA domain-containing protein ... 80 3e-16 gi|300312001|ref|YP_003776093.1| HipA-like protein [Herbaspirill... 73 5e-14 >gi|300309753|ref|YP_003773845.1| HipA domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 455 Score = 410 bits (1055), Expect = e-116, Method: Compositional matrix adjust. Identities = 224/445 (50%), Positives = 288/445 (64%), Gaps = 8/445 (1%) Query: 3 RRTLTQRLFVWMNGIRVAAWDKTRDEERLSYFPEWQEQEEGRPLSLSLPFTPNNLVYRGA 62 RR + L +WMNG V W T + L Y W GRPLSLSLPF P N +RG Sbjct: 5 RRRKPKELGLWMNGSFVGTWALTAQGDSLQYDARWVVSASGRPLSLSLPFRPGNPPHRGD 64 Query: 63 LVSSFFDNLLPDNDAIRRRLAQRYHAENTDPFSLLAELGRDCVGAIQLLPPDVAPTDLFS 122 LV +F+NLLPD+ AIR RLA+RY A +TD F+LL E GRDCVGA+Q+LP P ++ Sbjct: 65 LVRHYFENLLPDSKAIRERLARRYQAADTDAFALLGEAGRDCVGALQILPLGATPELSWT 124 Query: 123 ISGRPLDEAEVANLLRNTTSAAILGQTGEHDDLRLSIAGAQEKTALLYHEGAWHLPQGST 182 I RPL E EVA LLR T L T + ++LRLSIAGAQEKTALL + W LP G+T Sbjct: 125 IDARPLREREVAELLRQTMEPTPLALT-QGEELRLSIAGAQEKTALLRYLDQWCLPHGAT 183 Query: 183 PTTHIFKLPLGLVGNRRADMQSSVENEWLCSKLMAAYELPIAHCDIGRFEDQKVLIVERF 242 PTTHI KLP+GLVG + D++ SVENEWLCS+++AAY +P+A CDI FEDQKVL VERF Sbjct: 184 PTTHILKLPMGLVGGLQLDLRHSVENEWLCSRILAAYGMPVAACDIFCFEDQKVLAVERF 243 Query: 243 DRK----PASDGSWIIRLPQEDMCQATGTPSLRKYQADGGPGIADVIHVLDRSDKAQQDK 298 DR+ PAS + ++RLPQEDMCQATG KY+ADGGPG+ +++ +L + + Sbjct: 244 DRRWSRDPASGQTMLLRLPQEDMCQATGISPWLKYEADGGPGMKEIMALLGGAIDPVAAR 303 Query: 299 RNFFKTQIVFWLLAATDGHAKNFSIAHLPGGSYCATPLYDVLSAHPITGPGPNQMAAQRL 358 R+FFK Q++FW+L ATDGHAKNFS+ GG Y TPLYDVLS P+ G GP +++ R Sbjct: 304 RHFFKAQLLFWMLWATDGHAKNFSLFLKAGGRYEPTPLYDVLSVFPLIGEGPGKLSPHRA 363 Query: 359 KLAMAVRSTSNYYLIDRIQRRHWGAQAAQVGLGAAAAE---EIIEEVIAATDGVLDAVGR 415 +AMAVR+ + + + I RHW A + G+ A + I+EE+ T+ V+ V Sbjct: 364 AMAMAVRAKNAHRKMKDILPRHWMALGSHFGIVAENGDGPLAILEELAEQTEQVIAKVQA 423 Query: 416 QLPEDFPAKLADVIFSGVARQCKRL 440 LP FP++LA I G+ R L Sbjct: 424 DLPPGFPSELAASILDGLRRSASSL 448 >gi|300313385|ref|YP_003777477.1| HipA domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 439 Score = 80.5 bits (197), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 117/448 (26%), Positives = 177/448 (39%), Gaps = 83/448 (18%) Query: 23 DKTRDEERLSYFPEWQEQEEGRPLSLSLPFTPNNLVYRGALVSSFFDNLLPDNDAIRRRL 82 D + Y+ WQ E PLS + ++ + ++F NL P+ DA R+ Sbjct: 22 DIQSSDFNFYYYAHWQLSTETFPLSPHFALSQGR--FKSSATANFMMNLFPEGDA---RM 76 Query: 83 AQRYHAE-NTDP-FSLLAELGRDCVGAIQLL--------PPDVAPTDLFSISGRPLDEAE 132 A H+ N D FS+ G + GA L P AP R +D+ E Sbjct: 77 AIMKHSRLNADNVFSMAYLFGAEPAGAASFLFHSGTSALPSSQAPYL------REIDDDE 130 Query: 133 VANLLRNTTSAAILGQTGEHDDLRLSIAGAQEKTALLYHEGAWHLPQGSTPTTHIFKLPL 192 ++ + Q H S+AG Q+K +++H+ L GS +THI K Sbjct: 131 LSERIHARHRGVPFAQW--HGAWHQSLAGVQDKLQVIFHDERLFLAGGSLTSTHILKPEP 188 Query: 193 GLVGNRRADMQSSVENEWLCSKLMAAYELPIAHCDIGRFEDQKVLIVERFDRK--PASDG 250 G R + V NE C L AA L + H I R + VL+++RFDR P +D Sbjct: 189 Q--GERTPHL---VANEHFCMSLAAAVGLSVPHVKIHR-APEAVLLIDRFDRTYAPGTDV 242 Query: 251 SWIIRLPQE---DMCQATGTPSLRKYQADGGPGIADVIHVLDRSDKAQQ--DKRNFF--- 302 R+ + D CQA PS KY+ GG +RS + + D + F Sbjct: 243 LQPGRVERTHIIDGCQALELPSWYKYECLGGHQ--------NRSPERRYGIDFKRLFGLA 294 Query: 303 ---------KTQIVFW-----LLAATDGHAKNFSIAHLPGGSYCATPLYDVLSAHPITGP 348 K IV W L+ +D H KN S G A P YD++S Sbjct: 295 PHFEDASAAKLLIVRWALFNLLIGNSDAHGKNISFRQTANGIMPA-PFYDLVSV------ 347 Query: 349 GPNQMAAQRLKLAMAVRSTSNYYLIDRIQRRHWGAQAAQVGLGAAAAEEIIEEVIAATDG 408 Q+ +++ MA+ + + +L +++ A A G+ IE+V+ G Sbjct: 348 ---QVYGEKVLQEMAM-AFGDQFLAEKVGVADLAAFAQVAGIP-------IEDVMGELTG 396 Query: 409 VLDAVGRQLPEDFPAKLADVIFSGVARQ 436 + AV E PA AD + R+ Sbjct: 397 IAAAV----IEMAPALAADACYLDAERK 420 >gi|300312001|ref|YP_003776093.1| HipA-like protein [Herbaspirillum seropedicae SmR1] Length = 446 Score = 73.2 bits (178), Expect = 5e-14, Method: Compositional matrix adjust. Identities = 111/386 (28%), Positives = 159/386 (41%), Gaps = 77/386 (19%) Query: 60 RGALVSSFFDNLLPDNDAIRRRLAQRYHAENTDPFSLLAELGRDCVGAIQLLPPDVAPTD 119 RG L FF NLLP+ +R+ L QR D F LLA GRD LP DV Sbjct: 74 RGGL-PPFFQNLLPEGQ-LRKHLVQRAGLAPDDEFGLLAYCGRD-------LPGDV---- 120 Query: 120 LFSISGRPLDEAEVANLL------RNTTSAAILGQTGEHDDLRLSIAGAQEKTALLYHEG 173 S S LDE + LL +S + GE S++G Q K AL+ G Sbjct: 121 --SASAEELDERGLGRLLGQGHDSYEMSSGQLPVPGGE------SLSGVQPKLALVREAG 172 Query: 174 AWHL--PQGSTPTTHIFKLPLGLVGNRRADMQSSVENEWLCSKLMAAYEL--------PI 223 ++ + S + I KLP D + + E+ L AA + P+ Sbjct: 173 GRYVMRSKDSHGSHFIAKLP-------ATDYPNMPQVEFSSLTLAAAAGVHTCRFSLEPL 225 Query: 224 A----HCDIG-RFEDQKVLIVERFDRK-PASDGSWIIRLPQEDMCQATGTPSLRKYQADG 277 + H G R + + L+V+RFDR P G RL ED Q TGT KY Sbjct: 226 SAIAEHLPFGLRNDASRFLLVQRFDRDAPTPTG----RLHMEDFAQVTGTAPGAKYV--- 278 Query: 278 GPGIADVIHVLDRSDKAQQDKRNFFKTQIVFWLLAATDGHAKNFSIAHLPGGSYCATPLY 337 G A + +L+RS + +D + V LL D H KNFS+ + + +P Y Sbjct: 279 GSYAAIGVVLLERSTRGVEDVYELLRRIKVNELLGNFDAHLKNFSLLYHTPQAAALSPAY 338 Query: 338 DVLS-AHPITGPG------PNQMAAQRLKLAMAVRSTSNYY----------LIDRIQR-- 378 D+++ A + G G P Q Q L A+ +R +N + L+D ++R Sbjct: 339 DIVAYAAYVGGDGHALAFVPGQKGKQLLTPAI-LRQLANLWRIPEPKLQAVLVDTVERAM 397 Query: 379 RHWGAQAAQVGLGAAAAEEIIEEVIA 404 W A A + L A ++ + A Sbjct: 398 TQWPALIASLPLTDAQRTRLLSHLDA 423 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,454,241 Number of extensions: 63029 Number of successful extensions: 166 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 162 Number of HSP's successfully gapped: 3 Length of query: 447 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 359 Effective length of database: 1,203,190 Effective search space: 431945210 Effective search space used: 431945210 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)