BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0225.0034.p (187 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314078|ref|YP_003778170.1| uncharacterized protein involve... 155 2e-39 gi|300311615|ref|YP_003775707.1| outer membrane biogenesis prote... 70 8e-14 >gi|300314078|ref|YP_003778170.1| uncharacterized protein involved in outer membrane biogenesis protein [Herbaspirillum seropedicae SmR1] Length = 682 Score = 155 bits (392), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 81/161 (50%), Positives = 101/161 (62%), Gaps = 6/161 (3%) Query: 1 MPRTSKIIGFSLAGVIALIVLLVILIITFDWNRARPWINQRVSDATGRHFAIQGDLVVKW 60 MPR +KII + A +I LI VI I++FDWNRA+PWIN+RVS + GR FAI GDL + W Sbjct: 1 MPRLTKIILWLCASLILLITAAVIFILSFDWNRAKPWINERVSASIGREFAIDGDLSLHW 60 Query: 61 -RAPDSPQTS----WARWIPWPHVSANDIVLGNPDWAPANSNMATVKQLNVTLNPWPLLQ 115 RA P + W RW+PWP + A + +GNP MAT+ Q+ TLNP PLL Sbjct: 61 QRAQTLPGAAPPGFWERWLPWPRLQAEQLRIGNPPAFSQAGQMATIGQVTFTLNPLPLLV 120 Query: 116 HKIVLPDLEAQDPYLQLLRRKDAVNNWTFKSDDS-PSTWQF 155 H I LP L+ +DP + LLR AVNNWTFKS + PS W Sbjct: 121 HDIALPSLDIRDPSVSLLRNAQAVNNWTFKSSSAGPSAWNL 161 >gi|300311615|ref|YP_003775707.1| outer membrane biogenesis protein [Herbaspirillum seropedicae SmR1] Length = 654 Score = 70.5 bits (171), Expect = 8e-14, Method: Compositional matrix adjust. Identities = 42/128 (32%), Positives = 63/128 (49%), Gaps = 3/128 (2%) Query: 30 DWNRARPWINQRVSDATGRHFAIQGDLVVKWR--APDSPQTSWARWIPWPHVSANDIVLG 87 DWN A+PW+ +++S A GR + G+L + W D + AR P + A + +G Sbjct: 41 DWNSAKPWLGRQLSAALGRDIRVDGNLDLSWSLAQADDEAGALARLAPQLRLRAERLFIG 100 Query: 88 NPDWAPANSNMATVKQLNVTLNPWPLLQHKIVLPDLEAQDPYLQLLRRKDAVNNWTFKSD 147 NPDWA ++ ++ L+V +P PLL + DL + L + R D NW F S Sbjct: 101 NPDWAHSSDHLVRAAALDVRFSPLPLLHWHWHVTDLRVEGLDLVMERADDQRKNWRFSSQ 160 Query: 148 DSPSTWQF 155 S W F Sbjct: 161 PR-SRWSF 167 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.135 0.446 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 607,977 Number of extensions: 23844 Number of successful extensions: 49 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 47 Number of HSP's successfully gapped: 2 Length of query: 187 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 107 Effective length of database: 1,241,070 Effective search space: 132794490 Effective search space used: 132794490 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 128 (53.9 bits)