BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0225.0033.p
         (354 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300314078|ref|YP_003778170.1| uncharacterized protein involve...   459   e-130
gi|300311615|ref|YP_003775707.1| outer membrane biogenesis prote...   219   2e-58

>gi|300314078|ref|YP_003778170.1| uncharacterized protein involved
           in outer membrane biogenesis protein [Herbaspirillum
           seropedicae SmR1]
          Length = 682

 Score =  459 bits (1182), Expect = e-130,   Method: Compositional matrix adjust.
 Identities = 216/320 (67%), Positives = 274/320 (85%)

Query: 1   VVSNLLRFEDLGPLIGADSNASKEKRGAEERQPADKVLPVKEFQTEAWGAIDADVKLTAH 60
           + S +LRF+DL PLIGADSNA K +RGA+ERQP DKVLPVK F T+AWGA+DADVKLT  
Sbjct: 341 LTSEVLRFDDLAPLIGADSNAEKAERGAQERQPGDKVLPVKTFSTDAWGALDADVKLTGK 400

Query: 61  RVVKEKDLPVNNIQADLHLKDKVLSLTPLNFGIAGGNLTSNMRLDGREPKMKSDVKLSAR 120
           R++K+K+LP++++QA L LKDKVL+L PL+FG+AGGNL S +RLDG+   MKS++ LSAR
Sbjct: 401 RIIKQKNLPISDLQAHLILKDKVLTLAPLDFGVAGGNLRSTIRLDGQAAPMKSELILSAR 460

Query: 121 RIKIQQLFPNLNAAQTSFGEINGDAALTATGNSVAAMLGSSNGELKTLINHGAISKLLLE 180
           R+KIQ+L+P L+A++TSFGEINGDA L+ATGNSVAAMLGSSNGE+KTL+  GAISK LLE
Sbjct: 461 RLKIQKLYPQLDASRTSFGEINGDARLSATGNSVAAMLGSSNGEVKTLVTQGAISKFLLE 520

Query: 181 EMGLNIGNVVVSKLFGDKQVKLNCMAGDFTVTNGLAQARTFTLDTEDAIITITGDVNLAQ 240
             GLN+GNVV+SKLFGDK+VKLNC+AGD  V  G+  ART  LDTEDA+I++ G +++ +
Sbjct: 521 AAGLNLGNVVISKLFGDKEVKLNCLAGDLPVREGIMTARTLMLDTEDALISVDGTIDMRR 580

Query: 241 EQLNLTINPRSKGLRIISLRSPLYVTGPFKDPNVGVDKGVLALKTGGAIALGVLAPVAAI 300
           E+L+L ++P+SKGLRIISLRSPLYV G FK P+VGVDKG+LAL+ G A+ALG+LAPV AI
Sbjct: 581 EKLDLHVHPKSKGLRIISLRSPLYVAGTFKQPDVGVDKGMLALRAGSAVALGLLAPVTAI 640

Query: 301 LPLINISGEQQTDCANLLQE 320
           LPLIN+ G++Q DCA+LL+E
Sbjct: 641 LPLINVDGDEQADCAHLLEE 660


>gi|300311615|ref|YP_003775707.1| outer membrane biogenesis protein
           [Herbaspirillum seropedicae SmR1]
          Length = 654

 Score =  219 bits (559), Expect = 2e-58,   Method: Compositional matrix adjust.
 Identities = 126/282 (44%), Positives = 180/282 (63%)

Query: 34  ADKVLPVKEFQTEAWGAIDADVKLTAHRVVKEKDLPVNNIQADLHLKDKVLSLTPLNFGI 93
           AD+  P K  Q E W  +DAD+ L A RV     L      A L LKD+VL+  PL F +
Sbjct: 369 ADQGKPSKPAQPERWSKLDADIDLQAKRVQWRAGLEWQQAAATLTLKDQVLTADPLRFNM 428

Query: 94  AGGNLTSNMRLDGREPKMKSDVKLSARRIKIQQLFPNLNAAQTSFGEINGDAALTATGNS 153
           AGG     +RLDGR+P ++S +++  +  ++++LFP L + + SF + NG+A L+ATGNS
Sbjct: 429 AGGQGQGQVRLDGRQPDIRSRLQIRLQDAQVRELFPRLASLKASFSKFNGEATLSATGNS 488

Query: 154 VAAMLGSSNGELKTLINHGAISKLLLEEMGLNIGNVVVSKLFGDKQVKLNCMAGDFTVTN 213
            AA+L S++GE+K  +  G IS+ +LE  GLN+ N V +KL+ D+QVKL C A D  + +
Sbjct: 489 AAAVLASADGEIKADLGPGTISQFILEAAGLNLANAVFAKLYRDQQVKLLCGAADVAIQH 548

Query: 214 GLAQARTFTLDTEDAIITITGDVNLAQEQLNLTINPRSKGLRIISLRSPLYVTGPFKDPN 273
           G A  R   L+TEDA I + G V+L ++ L L I+PR+K +RI+SLR+PL+V G F  P 
Sbjct: 549 GQATIRHGILNTEDAAIDVGGQVDLGRQTLALDIHPRTKQVRILSLRTPLHVRGSFSHPE 608

Query: 274 VGVDKGVLALKTGGAIALGVLAPVAAILPLINISGEQQTDCA 315
           +G D G LA + G A AL ++APVAA++PLI+       DCA
Sbjct: 609 IGADSGALAARAGAAAALALVAPVAAVIPLISPGQAASDDCA 650


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.314    0.132    0.362 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,034,889
Number of extensions: 41992
Number of successful extensions: 93
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 93
Number of HSP's successfully gapped: 2
Length of query: 354
Length of database: 1,619,870
Length adjustment: 86
Effective length of query: 268
Effective length of database: 1,212,660
Effective search space: 324992880
Effective search space used: 324992880
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 131 (55.1 bits)