BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0225.0031.p (458 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314075|ref|YP_003778167.1| methylmalonate-semialdehyde deh... 685 0.0 gi|300313930|ref|YP_003778022.1| methylmalonate-semialdehyde deh... 526 e-150 gi|300312371|ref|YP_003776463.1| methylmalonate-semialdehyde deh... 342 3e-95 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 166 4e-42 gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrog... 159 3e-40 >gi|300314075|ref|YP_003778167.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 503 Score = 685 bits (1767), Expect = 0.0, Method: Compositional matrix adjust. Identities = 364/446 (81%), Positives = 391/446 (87%), Gaps = 10/446 (2%) Query: 8 IQHYINGRIAPSESGRRQDVFNPATGEVSGQVVLASTAEVNAAVASASAAAPGWAETAPL 67 I H+ING+I+ SESGR QDVFNPATG + +V LAS EVNAAVA+ASAAAP WAETAPL Sbjct: 6 IDHFINGQISRSESGRSQDVFNPATGAATAKVALASVQEVNAAVAAASAAAPAWAETAPL 65 Query: 68 KRARVLFKFKELIEQHHDDLAAAITREHGKVFSDAKGEVTRGLEIVEFACGVPQLLKTQF 127 KRARVLFKFKELIEQ+HD LA AIT EHGKVFSDAKGEVTRGLEIVEFA G+PQLLKTQF Sbjct: 66 KRARVLFKFKELIEQNHDALAKAITLEHGKVFSDAKGEVTRGLEIVEFATGIPQLLKTQF 125 Query: 128 TDNIGGGIDNWNLRQRWASPPASRRSISPSWCRCGWRRWRSLPATASSSS---RPSA--- 181 TDNIGGGIDNWNLRQ P I+P W + A A+ +S +PS Sbjct: 126 TDNIGGGIDNWNLRQ----PLGVTAGITPFNFPVMVPLWMAPIAIATGNSFVLKPSERDP 181 Query: 182 IXSLMLADLFKRAGLPDGVFNVVQGDKVAVDALLQHPEVQAISFVGSTPIAEYIYSEATK 241 SLMLA+LFKRAGLPDGVFNVVQGDKVAVDALL+HPEVQA+SFVGSTPIAEYIY +AT+ Sbjct: 182 SPSLMLAELFKRAGLPDGVFNVVQGDKVAVDALLEHPEVQAVSFVGSTPIAEYIYRKATE 241 Query: 242 RAGTFPLRAQALGGAKNHLVVMPDANLEQAVDALIGAAYGSAGERCMAISVAVAVGSVAD 301 RAGTFPLRAQALGGAKNHLVVMPDANL+QAVDALIGAAYGSAGERCMAISVAVAVGSVAD Sbjct: 242 RAGTFPLRAQALGGAKNHLVVMPDANLDQAVDALIGAAYGSAGERCMAISVAVAVGSVAD 301 Query: 302 KLVEALIPRVKALKIKNGMEADAEMGPLVTAAHKAKVEGYIDDGVNAGAKLLVDGRGIKV 361 KLVEALIPRVKALK+KNGME DAEMGPLVTAAHKAKVEGYI GV+AGAKLLVDGRG+KV Sbjct: 302 KLVEALIPRVKALKVKNGMEDDAEMGPLVTAAHKAKVEGYIQSGVDAGAKLLVDGRGLKV 361 Query: 362 PGHENGFYVGGTLFDHVTPDMKIYKEEIFGPVLCVVRVPDFAEAVRLINAHEYGNGVSLF 421 PGHE GF++GGTLFDHVT +MKIY+EEIFGPVLCVVRVPDFA AV+LINAHEYGNGVSLF Sbjct: 362 PGHEEGFFIGGTLFDHVTTEMKIYQEEIFGPVLCVVRVPDFASAVQLINAHEYGNGVSLF 421 Query: 422 TADGNTAHEFSRRIQVGMVGINVPXP 447 TADGNTAHEFSRRIQVGMVGINVP P Sbjct: 422 TADGNTAHEFSRRIQVGMVGINVPIP 447 >gi|300313930|ref|YP_003778022.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 497 Score = 526 bits (1356), Expect = e-150, Method: Compositional matrix adjust. Identities = 272/449 (60%), Positives = 323/449 (71%), Gaps = 20/449 (4%) Query: 8 IQHYINGRIAPSESGRRQDVFNPATGEVSGQVVLASTAEVNAAVASASAAAPGWAETAPL 67 I HYI G+ + SGR DVFNPA G QV L ++ EV+AAV + AA P W+ T PL Sbjct: 4 IAHYIGGQRVDTRSGRYADVFNPAQGVPVAQVALGTSDEVDAAVKAGVAAFPAWSNTPPL 63 Query: 68 KRARVLFKFKELIEQHHDDLAAAITREHGKVFSDAKGEVTRGLEIVEFACGVPQLLKTQF 127 RARVLF+F LI+Q DD A I REHGK FSDA+GEV RG+EIVEFA G+PQ+LK ++ Sbjct: 64 TRARVLFRFLHLIQQRADDFARIIVREHGKTFSDAQGEVARGIEIVEFAAGIPQMLKGEY 123 Query: 128 TDNIGGGIDNWNLRQRWAS----PPASRRSISPSW-----CRCGWRRWRSLPATASSSSR 178 TD I GID W++RQ P + ++ P W CG + S Sbjct: 124 TDQIARGIDAWSMRQALGVVAGITPFNFPAMVPMWMFPIAIACG-----NCFVLKPSERD 178 Query: 179 PSAIXSLMLADLFKRAGLPDGVFNVVQGDKVAVDALLQHPEVQAISFVGSTPIAEYIYSE 238 PSA SL++ADL K AGLPDGVFNVVQGDKV VDALL HP+V+AISFVGSTPIAEYIY+ Sbjct: 179 PSA--SLLIADLLKEAGLPDGVFNVVQGDKVTVDALLDHPQVKAISFVGSTPIAEYIYAR 236 Query: 239 ATKRAGTFPLRAQALGGAKNHLVVMPDANLEQAVDALIGAAYGSAGERCMAISVAVAVGS 298 + + R QALGGAKNH+VVMPDA+++ AVDAL+GAAYGSAGERCMAISVA+AVGS Sbjct: 237 GSAKGK----RVQALGGAKNHMVVMPDADMQLAVDALMGAAYGSAGERCMAISVALAVGS 292 Query: 299 VADKLVEALIPRVKALKIKNGMEADAEMGPLVTAAHKAKVEGYIDDGVNAGAKLLVDGRG 358 D+LV AL R + LKI GM A AEMGP+VTAA K ++EG I GV GA L+VDGRG Sbjct: 293 AGDELVAALAERARKLKINEGMAAGAEMGPVVTAAAKERIEGLIGRGVEEGATLVVDGRG 352 Query: 359 IKVPGHENGFYVGGTLFDHVTPDMKIYKEEIFGPVLCVVRVPDFAEAVRLINAHEYGNGV 418 +VPG E GF+VGGTL DHVTP+M +YKEEIFGPVLCV+R PD A AV LINAHEYGNGV Sbjct: 353 YQVPGFEKGFFVGGTLLDHVTPEMTVYKEEIFGPVLCVLRCPDIASAVELINAHEYGNGV 412 Query: 419 SLFTADGNTAHEFSRRIQVGMVGINVPXP 447 +++T DG A EF R+I+VGMVG+NVP P Sbjct: 413 AVYTRDGGVAREFVRQIEVGMVGVNVPLP 441 >gi|300312371|ref|YP_003776463.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 342 bits (878), Expect = 3e-95, Method: Compositional matrix adjust. Identities = 191/446 (42%), Positives = 265/446 (59%), Gaps = 21/446 (4%) Query: 11 YINGRIAPSESGRRQDVFNPATGEVSGQVVLASTAEVNAAVASASAAAPGWAETAPLKRA 70 Y+NG S S +DV NPAT EV +V A+ EV+ AVA+A W T+ +R Sbjct: 12 YLNGEKVQSTSKEWRDVLNPATQEVVARVPFATKEEVDRAVANAKETFKTWRNTSLAQRM 71 Query: 71 RVLFKFKELIEQHHDDLAAAITREHGKVFSDAKGEVTRGLEIVEFACGVPQLLKTQFTDN 130 R++ KF++L+ ++ LA ITREHGK DA+GEV RGLE+VE AC + L + +N Sbjct: 72 RIMLKFQQLLRENIAPLAELITREHGKTLPDAEGEVMRGLEVVEHACSITSLQLGELAEN 131 Query: 131 IGGGIDNWNLRQ---------RWASPPASRRSISPSWCRCGWRRWRSLPATASSSSRPSA 181 + GG+D + L Q + P + P CG + S P++ Sbjct: 132 VAGGVDVYTLYQPLGVGAGITAFNFPVMLPCFMFPIAVACG-----NTFVLKPSEQDPTS 186 Query: 182 IXSLMLADLFKRAGLPDGVFNVVQGDKVAVDALLQHPEVQAISFVGSTPIAEYIYSEATK 241 SL L +L +AGLP GV NVV G + + HP+++A+SF+GST + +IY + Sbjct: 187 --SLFLVELANQAGLPPGVLNVVHGGPDVANMICDHPDIKAVSFIGSTHVGTHIY----R 240 Query: 242 RAGTFPLRAQALGGAKNHLVVMPDANLEQAVDALIGAAYGSAGERCMAISVAVAVGSVAD 301 RA RAQ + GAKNH +V+PDA +QA++ L+GAA+G+AG+RCMA SV V VG + Sbjct: 241 RASEAGKRAQCMMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCMANSVVVLVGQTRE 300 Query: 302 KLVEALIPRVKALKIKNGMEADAEMGPLVTAAHKAKVEGYIDDGVNAGAKLLVDGRGIKV 361 + E ++ R KA+K+ G + A++GPLV+ A K +VE I GV GAKLL+DGR KV Sbjct: 301 WIPE-IVERSKAMKVGPGTDRKADVGPLVSKAAKERVERLIASGVEQGAKLLLDGRNCKV 359 Query: 362 PGHENGFYVGGTLFDHVTPDMKIYKEEIFGPVLCVVRVPDFAEAVRLINAHEYGNGVSLF 421 G ENG +VG T+F V P+M IY +EIFGP +C+V + EA+ INA+ GNG S+F Sbjct: 360 AGSENGNFVGPTVFTGVKPEMDIYTQEIFGPAMCIVELDTLDEAIAFINANPNGNGTSIF 419 Query: 422 TADGNTAHEFSRRIQVGMVGINVPXP 447 T+ G A +F I VG VGINVP P Sbjct: 420 TSSGYAARKFQNEIDVGQVGINVPIP 445 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 166 bits (420), Expect = 4e-42, Method: Compositional matrix adjust. Identities = 145/469 (30%), Positives = 220/469 (46%), Gaps = 46/469 (9%) Query: 1 MTAAPST-------IQH-YINGRIAPSESGRRQDVFNPATGEVSGQVVLASTAEVNAAVA 52 MT++P T + H YI+G+ P + R V PAT E ++ L + A+V+ AV Sbjct: 1 MTSSPLTHLTRNARLDHFYIDGKWQPPQGSERAAVVAPATEERVCEIALGNAADVDQAVQ 60 Query: 53 SASAAAPGWAETAPLKRARVLFKFKELIEQHHDDLAAAITREHGKVFSDAK-GEVTRGLE 111 +A A P WA T+PL+RA +L + L+ + + A A+T E G S A+ V E Sbjct: 61 AARRAIPAWAATSPLERAALLGRVHALLLERSELFAQALTLEMGAPISYARTAHVPLAAE 120 Query: 112 IVEFACGVPQLLKTQFTDNIGGGIDNWNLRQRWAS----PPASRRSISPSWCRCGWRRWR 167 + A DN+ D +R R + P ++ W W ++ Sbjct: 121 HLRVA-----------RDNLA---DYPFIRPRGTTAIVREPIGVCALITPW---NWPIYQ 163 Query: 168 SL----PATAS------SSSRPSAIXSLMLADLFKRAGLPDGVFNVVQGDKVAVDALLQ- 216 PA A+ S S + +L+ A++ AG+P GVFN+V G V A L Sbjct: 164 ITAKVGPALAAGCTVVLKPSELSPLSALLFAEIVHEAGVPAGVFNLVNGSGAEVGAALSA 223 Query: 217 HPEVQAISFVGSTPIAEYIYSEATKRAGTFPLRAQALGGAKNHLVVMPDANLEQAVDALI 276 HP+V IS GST + A A T AQ LGG +LV +PDA+LE+A+ + Sbjct: 224 HPQVDMISITGSTRAGVLV---AQAAAVTVKRVAQELGGKSPNLV-LPDADLERAIPPGV 279 Query: 277 GAAYGSAGERCMAISVAVAVGSVADKLVEALIPRVKALKIKNGMEADAEMGPLVTAAHKA 336 AA + G+ C A + + + ++ E + + +K+ + +A GPL A Sbjct: 280 AAALRNMGQSCSAPTRLIVPRAALARVHELALATLAQMKVGDPNDAATTHGPLANRAQFL 339 Query: 337 KVEGYIDDGVNAGAKLLVDGRGIKVPGHENGFYVGGTLFDHVTPDMKIYKEEIFGPVLCV 396 +V+ I+ G+ GA+LL G G + G G+Y T+F V DM I +EEIFGPVL + Sbjct: 340 RVQQMIEAGLQDGARLLAGGPG-RPDGLAQGYYARPTIFSEVHTDMVIAQEEIFGPVLAI 398 Query: 397 VRVPDFAEAVRLINAHEYGNGVSLFTADGNTAHEFSRRIQVGMVGINVP 445 + EA+ + N YG G + D + + RIQ G V +N P Sbjct: 399 LPYDTVEEAIAIANDTVYGLGAHVQGRDKDQVRAVAARIQSGQVHLNYP 447 >gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 480 Score = 159 bits (403), Expect = 3e-40, Method: Compositional matrix adjust. Identities = 130/452 (28%), Positives = 209/452 (46%), Gaps = 37/452 (8%) Query: 8 IQHYINGRIAPSESGRRQDVFNPATGEVSGQVVLASTAEVNAAVASASAAAPGWAETAPL 67 +Q YI+GR E R Q+V +PATG+V G++ A A++ A+ +A A W + +PL Sbjct: 5 LQLYIDGRFHGVEGRRYQEVRDPATGDVLGRLPWAERADIEEAIQAAHRAFQQWRQVSPL 64 Query: 68 KRARVLFKFKELIEQHHDDLAAAITREHGKVFSDAKGEVTRGLEIVEF------------ 115 +R+ +L + L + +A AIT ++GK +DA E+ E +++ Sbjct: 65 ERSDILRRAAALARERAQAMAPAITMDNGKPLADAVAEIINAAEHLDWHAEEGRRLYGRV 124 Query: 116 -ACGVPQLLKTQFTDNIG--GGIDNWNLRQRWASPPASRRSISPSWCRCGWRRWRSLPAT 172 P++ + + +G I WN + + + C + Sbjct: 125 VGARHPEVRQLVVREPVGVCAAISPWNFP--FGQAMKKVAAAMAAGCTVVLK-------- 174 Query: 173 ASSSSRPSAIXSLMLADLFKRAGLPDGVFNVVQGDKVAVDA-LLQHPEVQAISFVGSTPI 231 S P+AI +++A LF AG+P GVFN+V G + L++ P V+ ISF GS P+ Sbjct: 175 -GPSESPAAI--VLMAQLFHDAGVPAGVFNIVSGVSSEISQHLIESPLVRNISFTGSVPV 231 Query: 232 AEYIYSEATKRAGTFPLRAQALGGAKNHLVVMPDANLEQAVDALIGAAYGSAGERCMAIS 291 + ++A + LGG ++V DA +E A L +AG+ C++ S Sbjct: 232 GSKLAAQAALHMKRMSME---LGGHAP-VLVFDDAEVEPAARLLARLKTRNAGQVCVSPS 287 Query: 292 VAVAVGSVADKLVEALIPRVKALKIKNGMEADAEMGPLVTAAHKAKVEGYIDDGVNAGAK 351 + + V A + +K+ G E +MGPL+ V+G IDD + G++ Sbjct: 288 RFYVQKGIYARFVTAFAEALLQIKVGPGHEPGVQMGPLIHGRRFEAVQGLIDDALAQGSE 347 Query: 352 LLVDGRGIKVPGHENGFYVGGTLFDHVTPDMKIYKEEIFGPVLCVVRVPDFAEAVRLINA 411 L+ G + GH FY TL V P ++ +EE FGP+ V EA+ + NA Sbjct: 348 LVTGGARLGQRGH---FY-APTLITGVPPSARVMQEEPFGPIAAVTPFSQAEEAIAMANA 403 Query: 412 HEYGNGVSLFTADGNTAHEFSRRIQVGMVGIN 443 G +FT +TAH SR +Q GMV IN Sbjct: 404 LPLGLASYVFTQSLDTAHRVSRDLQAGMVNIN 435 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,229,026 Number of extensions: 46170 Number of successful extensions: 219 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 180 Number of HSP's successfully gapped: 24 Length of query: 458 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 370 Effective length of database: 1,203,190 Effective search space: 445180300 Effective search space used: 445180300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)