BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0225.0029.p (159 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314073|ref|YP_003778165.1| amino acid ABC transporter ATPa... 291 1e-80 gi|300311565|ref|YP_003775657.1| branched-chain amino acid ABC t... 114 4e-27 gi|300310523|ref|YP_003774615.1| branched-chain amino acid ABC t... 108 2e-25 gi|300309521|ref|YP_003773613.1| branched-chain amino acid ABC t... 108 2e-25 gi|300312239|ref|YP_003776331.1| amino acid ABC transporter ATPa... 107 4e-25 >gi|300314073|ref|YP_003778165.1| amino acid ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 291 bits (746), Expect = 1e-80, Method: Compositional matrix adjust. Identities = 137/159 (86%), Positives = 152/159 (95%) Query: 1 MENVRIGLQRTLGTSFHFWKSSDSLKQLNDRAMALLDEVDLTSFADTLTVDLPYGRKRAL 60 +ENVRIGLQR LGTSFHFWK+ +SLKQL++RAM LL+EVDLTSFA T TVDLPYGRKRAL Sbjct: 98 LENVRIGLQRKLGTSFHFWKNGESLKQLDERAMQLLEEVDLTSFAHTTTVDLPYGRKRAL 157 Query: 61 EIATTLAMEPELMLLDEPTQGMGHEDVDRVTALIKKVSAGRTILMVEHNMNVIAGICDRI 120 EIATTLAMEPELMLLDEPTQGMGHEDVDRVTALIKKVSAGRTILMVEHNM+VIAGICDRI Sbjct: 158 EIATTLAMEPELMLLDEPTQGMGHEDVDRVTALIKKVSAGRTILMVEHNMSVIAGICDRI 217 Query: 121 SVLQRGAVLAEGTYDQVSKNPQVMEAYMGTTAGALEGAH 159 SVLQRGA+LAEG+Y++VS+NPQVMEAYMG+T+ L+GAH Sbjct: 218 SVLQRGALLAEGSYEEVSRNPQVMEAYMGSTSAELQGAH 256 >gi|300311565|ref|YP_003775657.1| branched-chain amino acid ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 114 bits (285), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 58/148 (39%), Positives = 89/148 (60%) Query: 1 MENVRIGLQRTLGTSFHFWKSSDSLKQLNDRAMALLDEVDLTSFADTLTVDLPYGRKRAL 60 ENVR L TLG + FW+ + + RA+ +L +V L + AD +L Y +RAL Sbjct: 99 FENVRCALLWTLGYRYAFWRRMGGSHEASARALTVLQQVGLAARADVAAGELSYAEQRAL 158 Query: 61 EIATTLAMEPELMLLDEPTQGMGHEDVDRVTALIKKVSAGRTILMVEHNMNVIAGICDRI 120 E+A T+A + ++LLDEPT GM + + ALI++V+ G+T+LMVEH+M V+ + DRI Sbjct: 159 ELAVTIAADASVILLDEPTAGMSRAESAAMVALIREVTMGKTLLMVEHDMQVVFDLADRI 218 Query: 121 SVLQRGAVLAEGTYDQVSKNPQVMEAYM 148 +V+ G V+A T ++ N V Y+ Sbjct: 219 AVMDNGRVIACDTAPRIRANEDVQALYL 246 >gi|300310523|ref|YP_003774615.1| branched-chain amino acid ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 255 Score = 108 bits (270), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 59/155 (38%), Positives = 89/155 (57%), Gaps = 6/155 (3%) Query: 1 MENVRIGL-----QRTLGTSFHFWKSSDSLKQLNDRAMALLDEVDLTSFADTLTVDLPYG 55 +ENV +G Q G F + + + +++ LLD V + FA L YG Sbjct: 98 LENVMVGCHVRTKQNVFGAVFRHKAAREEEAAIREKSQKLLDFVGIGQFAKRTARHLSYG 157 Query: 56 RKRALEIATTLAMEPELMLLDEPTQGMGHEDVDRVTALIKKVSA-GRTILMVEHNMNVIA 114 +R LEIA LA +P+L+ LDEP GM + + L+ K+ A G+TIL++EH++ ++ Sbjct: 158 DQRRLEIARALATDPQLLALDEPAAGMNATEKLGLRELLVKIQAEGKTILLIEHDVKLMM 217 Query: 115 GICDRISVLQRGAVLAEGTYDQVSKNPQVMEAYMG 149 G+C+RI+VL G +AEG V KNP V+EAY+G Sbjct: 218 GLCNRITVLDYGKPIAEGVPADVQKNPAVIEAYLG 252 >gi|300309521|ref|YP_003773613.1| branched-chain amino acid ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 255 Score = 108 bits (270), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 59/155 (38%), Positives = 89/155 (57%), Gaps = 6/155 (3%) Query: 1 MENVRIGL-----QRTLGTSFHFWKSSDSLKQLNDRAMALLDEVDLTSFADTLTVDLPYG 55 +ENV +G Q G F + + + +++ LLD V + FA L YG Sbjct: 98 LENVMVGCHVRTRQNVFGAVFRHKAAREEEAAIREKSQKLLDFVGIGQFAKRTARHLSYG 157 Query: 56 RKRALEIATTLAMEPELMLLDEPTQGMGHEDVDRVTALIKKVSA-GRTILMVEHNMNVIA 114 +R LEIA LA +P+L+ LDEP GM + + L+ K+ A G+TIL++EH++ ++ Sbjct: 158 DQRRLEIARALATDPQLLALDEPAAGMNATEKLGLRELLVKIQAEGKTILLIEHDVKLMM 217 Query: 115 GICDRISVLQRGAVLAEGTYDQVSKNPQVMEAYMG 149 G+C+RI+VL G +AEG V KNP V+EAY+G Sbjct: 218 GLCNRITVLDYGKPIAEGVPADVQKNPAVIEAYLG 252 >gi|300312239|ref|YP_003776331.1| amino acid ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 253 Score = 107 bits (268), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 59/155 (38%), Positives = 89/155 (57%), Gaps = 6/155 (3%) Query: 1 MENVRIGLQRTLGTS-----FHFWKSSDSLKQLNDRAMALLDEVDLTSFADTLTVDLPYG 55 +ENV +G+ L + ++ K+ +RA+ LL V L A L L YG Sbjct: 95 LENVVVGMHDHLNYGVASMLLNLGGFRNAEKEARERALELLSWVRLDHKAHMLADSLSYG 154 Query: 56 RKRALEIATTLAMEPELMLLDEPTQGMG-HEDVDRVTALIKKVSAGRTILMVEHNMNVIA 114 +R LE A LA +P+L+LLDEP GM E + ++ ++ G TI M+EH+M + Sbjct: 155 EQRKLEFARALATKPKLLLLDEPVAGMNPAEKTELMSEIVNIKQRGFTIFMIEHDMRFVM 214 Query: 115 GICDRISVLQRGAVLAEGTYDQVSKNPQVMEAYMG 149 G+C+RI+VL G ++AEGT DQ+ N +V+EAY+G Sbjct: 215 GLCERIAVLNFGKIIAEGTPDQIKNNQEVIEAYLG 249 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.132 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 441,174 Number of extensions: 16213 Number of successful extensions: 278 Number of sequences better than 1.0e-08: 52 Number of HSP's gapped: 289 Number of HSP's successfully gapped: 53 Length of query: 159 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 81 Effective length of database: 1,250,540 Effective search space: 101293740 Effective search space used: 101293740 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)