BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0225.0027.p (403 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314071|ref|YP_003778163.1| amino acid ABC transporter peri... 705 0.0 gi|300309490|ref|YP_003773582.1| branched-chain amino acid ABC t... 581 e-167 gi|300312339|ref|YP_003776431.1| branched-chain amino acid ABC t... 558 e-160 gi|300312372|ref|YP_003776464.1| branched-chain amino acid ABC t... 364 e-102 gi|300313931|ref|YP_003778023.1| branched-chain amino acid ABC t... 98 1e-21 >gi|300314071|ref|YP_003778163.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 402 Score = 705 bits (1820), Expect = 0.0, Method: Compositional matrix adjust. Identities = 340/378 (89%), Positives = 360/378 (95%) Query: 26 QISGDVIKIGFITDISGVYSDIDGQGGAEAIKFAIADMGGSINGKKIELVVADHQNKADV 85 QISGDVIKIGFITDISGVYSDIDGQGGAEAIK AIADMGG+INGKKIEL+VADHQNKADV Sbjct: 25 QISGDVIKIGFITDISGVYSDIDGQGGAEAIKMAIADMGGAINGKKIELLVADHQNKADV 84 Query: 86 AASKSREWFDQQGVDVLIGGTNSGTALAMAKVANEKKKVFIENGAGSARLTNEECSPYTV 145 AASK+REWFDQQGVD+LIGGTNSGTALA AKVA EKKKV+++ GAGSARLTNEECSPYTV Sbjct: 85 AASKAREWFDQQGVDMLIGGTNSGTALASAKVAAEKKKVYMQIGAGSARLTNEECSPYTV 144 Query: 146 HYAYDTVALAKGTGSAVVKQGGKSWFFLTADYAFGHSLESDTSNVVKASGGTVVGSVKHP 205 HYAYDTVALAKGTGSAVVKQGGKSWFFLTADYAFGHSLESDT+NVVKASGGTVVG+VKHP Sbjct: 145 HYAYDTVALAKGTGSAVVKQGGKSWFFLTADYAFGHSLESDTANVVKASGGTVVGAVKHP 204 Query: 206 LGASDFSSFLLQAQTSKAQIIGLANAGGDFINSVKAANEFGITKTAKLAGLLVFINDVHS 265 LGASDFSSFLLQAQ+SKAQIIGLANAGGDFINSVKAANEFGITKTAKLAGLLVFINDVHS Sbjct: 205 LGASDFSSFLLQAQSSKAQIIGLANAGGDFINSVKAANEFGITKTAKLAGLLVFINDVHS 264 Query: 266 LTPKLTQGMYLTDSWYWDTNDETRKFAKRYFEKQKKMPSSLQAADYSAALQYLQAVKAAG 325 LT K T+G+YLTDSWYWD N+ETR FAKRYF +KKMPSSLQAADYSAALQYL+ VK G Sbjct: 265 LTLKNTEGLYLTDSWYWDANEETRAFAKRYFAVRKKMPSSLQAADYSAALQYLKVVKEIG 324 Query: 326 TDDSDKVMAQLRKTKFNDVFQKNGYLRADGRVVHDMYLYQVKTPAESKYPWDYYKLVQTI 385 TDDSDKVMA LRK KFND++QKNGYLRADGRV+HDMYLYQVK +ESKYPWDYYKLVQTI Sbjct: 325 TDDSDKVMAALRKAKFNDMYQKNGYLRADGRVMHDMYLYQVKAQSESKYPWDYYKLVQTI 384 Query: 386 PGEQAWTTKAESKCSAWK 403 P EQAWTTKAESKC+AWK Sbjct: 385 PAEQAWTTKAESKCAAWK 402 >gi|300309490|ref|YP_003773582.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 581 bits (1497), Expect = e-167, Method: Compositional matrix adjust. Identities = 275/393 (69%), Positives = 319/393 (81%) Query: 11 STAAAAFAFAAPASAQISGDVIKIGFITDISGVYSDIDGQGGAEAIKFAIADMGGSINGK 70 +TA A A SAQ +GD IKIGFITD+SG Y+D DGQGG EAI+ AIAD GG+INGK Sbjct: 12 ATACALLGAAILPSAQAAGDTIKIGFITDMSGTYADFDGQGGVEAIRMAIADAGGAINGK 71 Query: 71 KIELVVADHQNKADVAASKSREWFDQQGVDVLIGGTNSGTALAMAKVANEKKKVFIENGA 130 K+ELV ADHQNKAD+AA+K+REWFD+ GVD+L+GG NS +LAM KVA EKKKVFI GA Sbjct: 72 KVELVFADHQNKADIAANKAREWFDRDGVDMLVGGVNSAASLAMGKVAGEKKKVFISVGA 131 Query: 131 GSARLTNEECSPYTVHYAYDTVALAKGTGSAVVKQGGKSWFFLTADYAFGHSLESDTSNV 190 G+ R TNEEC+ YT+ YAYDT ALA+GTG+A+ +QGGKSW+FLTADYAFG SLE DTS V Sbjct: 132 GTTRQTNEECNAYTIQYAYDTTALARGTGAAITRQGGKSWYFLTADYAFGTSLEKDTSEV 191 Query: 191 VKASGGTVVGSVKHPLGASDFSSFLLQAQTSKAQIIGLANAGGDFINSVKAANEFGITKT 250 VK++GG VVG+ + PL SDFSSFLLQAQ+SKAQI+GLA AGGD IN++KAANEFG+ KT Sbjct: 192 VKSNGGNVVGATRVPLATSDFSSFLLQAQSSKAQILGLAVAGGDVINAIKAANEFGVNKT 251 Query: 251 AKLAGLLVFINDVHSLTPKLTQGMYLTDSWYWDTNDETRKFAKRYFEKQKKMPSSLQAAD 310 KLAGLL+FIND HSL LTQGMYLTD WYWD ND+TR +A RYF K KKMPS QA D Sbjct: 252 MKLAGLLIFINDTHSLGLSLTQGMYLTDGWYWDLNDQTRAWANRYFMKMKKMPSMFQAGD 311 Query: 311 YSAALQYLQAVKAAGTDDSDKVMAQLRKTKFNDVFQKNGYLRADGRVVHDMYLYQVKTPA 370 SA QYL+AVKA G+ D+DKVM LRK + ND F NG +R DGR+VHDMYL +VK P+ Sbjct: 312 ASAVGQYLKAVKAVGSTDADKVMDYLRKNRINDFFTSNGVVRPDGRMVHDMYLMEVKKPS 371 Query: 371 ESKYPWDYYKLVQTIPGEQAWTTKAESKCSAWK 403 ESK PWDYYKLVQTIPGEQA+ TKAESKC+ WK Sbjct: 372 ESKRPWDYYKLVQTIPGEQAYMTKAESKCALWK 404 >gi|300312339|ref|YP_003776431.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 558 bits (1438), Expect = e-160, Method: Compositional matrix adjust. Identities = 265/393 (67%), Positives = 325/393 (82%), Gaps = 3/393 (0%) Query: 13 AAAAFA--FAAPASAQISGDVIKIGFITDISGVYSDIDGQGGAEAIKFAIADMGGSINGK 70 AA A A F+ A AQ+S D ++IGFITD+SG Y+D DG GG EAI+ A+AD GG++ GK Sbjct: 12 AACAIAGIFSGAALAQVSNDAVRIGFITDMSGPYADTDGLGGLEAIRMAVADFGGTVLGK 71 Query: 71 KIELVVADHQNKADVAASKSREWFDQQGVDVLIGGTNSGTALAMAKVANEKKKVFIENGA 130 IE++ ADHQNKAD+AA+++REW D++G+D+LIGG NS TAL+M KV EKK+V+I GA Sbjct: 72 PIEVISADHQNKADIAATRAREWADERGLDMLIGGVNSATALSMNKVMAEKKRVYINIGA 131 Query: 131 GSARLTNEECSPYTVHYAYDTVALAKGTGSAVVKQGGKSWFFLTADYAFGHSLESDTSNV 190 G+ARLTNEEC+PYTVHY YDTVALAKGT SAV+KQGGKSWFFL ADYAFGHSL +DTS V Sbjct: 132 GTARLTNEECTPYTVHYEYDTVALAKGTASAVIKQGGKSWFFLVADYAFGHSLANDTSAV 191 Query: 191 VKASGGTVVGSVKHPLGASDFSSFLLQAQTSKAQIIGLANAGGDFINSVKAANEFGITKT 250 VKASGGTVVGS+KHP +SD SSFLLQAQ SKAQI+GLANAG D +NS+KAA EFG+TKT Sbjct: 192 VKASGGTVVGSIKHPPQSSDLSSFLLQAQASKAQILGLANAGEDTVNSIKAAKEFGVTKT 251 Query: 251 AKLAGLLVFINDVHSLTPKLTQGMYLTDSWYWDTNDETRKFAKRYFEKQKKMPSSLQAAD 310 KL GLL+ IND+H+L +G+ +TDSWYWD ++ +RKFA+R+F+K KKMPS+ QAA Sbjct: 252 MKLVGLLMVINDIHALGLANAEGLMMTDSWYWDKDEPSRKFAERFFQKMKKMPSTHQAAA 311 Query: 311 YSAALQYLQAVKAAGTDDSDKVMAQLRKTKFNDVFQKNGYLRADGRVVHDMYLYQVKTPA 370 YSA YL+AV+A GTDDS KVMAQL+KT+ +D + K G +RADGR +HDMYLYQVK+PA Sbjct: 312 YSATTTYLKAVQAIGTDDSTKVMAQLKKTRIDDFYNK-GSIRADGRNIHDMYLYQVKSPA 370 Query: 371 ESKYPWDYYKLVQTIPGEQAWTTKAESKCSAWK 403 ESK PWDY KL++TIPGEQA+T+ A+SKCS K Sbjct: 371 ESKKPWDYLKLLETIPGEQAFTSVADSKCSLLK 403 >gi|300312372|ref|YP_003776464.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 410 Score = 364 bits (934), Expect = e-102, Method: Compositional matrix adjust. Identities = 194/400 (48%), Positives = 264/400 (66%), Gaps = 11/400 (2%) Query: 1 MKLKAMSLAVSTAAAAF-----AFAAPASAQISGDVIKIGFITDISGVYSDIDGQGGAEA 55 M+ A+ +++ AA A A A PA AQ + +KIG +TD+SG YS + GQG A Sbjct: 1 MRKNALGRSLTAAAVACTVSLAATAIPAWAQ-DANTVKIGVLTDMSGPYSAMGGQGSVVA 59 Query: 56 IKFAIAD-MGGSINGKKIELVVADHQNKADVAASKSREWFDQQGVDVLIGGTNSGTALAM 114 K A+ D + G KIE++ AD+QNK DV +++REW D+ V+ + TNS ALA+ Sbjct: 60 AKMAVEDCLKNECKGMKIEILSADNQNKPDVGVTRAREWLDRDKVEAIADLTNSSVALAV 119 Query: 115 AKVANEKKKVFIENGAGSARLTNEECSPYTVHYAYDTVALAKGTGSAVVKQGGKSWFFLT 174 K+ +K + + +G + LT EC+P H+ +DT + A G +A+ K G KSW+F+T Sbjct: 120 QKLIKDKGGIAMYSGPATGALTTAECAPNGFHWMFDTYSQAAGAAAALTKLGNKSWYFVT 179 Query: 175 ADYAFGHSLESDTSNVVKASGGTVVGSVKHPLGASDFSSFLLQAQTSKAQIIGLANAGGD 234 DYAFGHSLE D S+VVKA+GGTVVGSV+HPL ASDFSSFLLQAQ SKAQ+IGLA+ G D Sbjct: 180 VDYAFGHSLEKDASDVVKANGGTVVGSVRHPLNASDFSSFLLQAQASKAQVIGLADGGTD 239 Query: 235 FINSVKAANEFGI-TKTAKLAGLLVFINDVHSLTPKLTQGMYLTDSWYWDTNDETRKFAK 293 +N++K A F + K +L LLVF++DVH+L + QG+ TD +YWD +++TR F+K Sbjct: 240 VVNAIKQARSFAVGGKDQRLVALLVFLSDVHALGLEAAQGLVYTDGFYWDYDEQTRAFSK 299 Query: 294 RYFEKQKK--MPSSLQAADYSAALQYLQAVKAAGTDDSDKVMAQLRKTKFNDVFQKNGYL 351 R FE Q K P+ +QA YS+ Q+L+A AA T D V ++R+ D +N + Sbjct: 300 R-FESQFKNIKPTMVQAGVYSSVYQFLKARAAAKTSDWKVVAQKMREIPIADAVMRNASI 358 Query: 352 RADGRVVHDMYLYQVKTPAESKYPWDYYKLVQTIPGEQAW 391 R DGRV+HDMYLYQVKTPAESK PWDY KL+ IP +QA+ Sbjct: 359 RPDGRVIHDMYLYQVKTPAESKGPWDYLKLLSVIPAQQAF 398 >gi|300313931|ref|YP_003778023.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 397 Score = 98.2 bits (243), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 98/381 (25%), Positives = 163/381 (42%), Gaps = 23/381 (6%) Query: 18 AFAAPASAQI-----SGDVIKIGFITDISGVYSDIDGQGGAEAIKFAIADMGGSINGKKI 72 A AAP + + I G +SG ++ +G+ +K A+ G + GK Sbjct: 12 ALAAPGALSYHDVFAQAESIAFGCPVPMSGPFA-ANGKFADLGMKLALQKYG-KVLGKST 69 Query: 73 ELVVADHQNKADVAASKSREWFDQQGVDVLIGGTNSGTALAMAKVANEKKKVFIENGAGS 132 V D + K A K +E ++G GG SG ALAM K A++ VFI AG+ Sbjct: 70 SYVNLDTEGKPATAVRKMQESI-EKGTRFFAGGILSGEALAMGKEADKAGAVFITT-AGA 127 Query: 133 ARLTNEECSPYTVHYAYDTVALAKGTGSAVVKQ--GGKSWFFLTADYAFGHSLESDTSNV 190 +T +C+ T ++ T + T ++++ K W+ +T Y FG L S N+ Sbjct: 128 DEITGSDCNRATFRWSVPTFGAIEQTVRPLLEKMPNAKRWYTITPQYVFGEGLLSAAKNI 187 Query: 191 VKASGGTVVGSVKHPLGASDFSSFLLQAQTSKAQIIGLANAGGDFINSVKAANEFGITKT 250 K G VG+ H L +FS +L A +K ++ L N G ++++ A FG+ Sbjct: 188 FKEKGIEHVGNSYHSLTEKEFSGYLTNAIAAKPDVLLLLNFGSQSSDTLRQAVSFGMKNN 247 Query: 251 AKL-----AGLLVFINDVHSLTPKLTQGMYLTDSWYWDTNDE--TRKFAKRYFEKQKKMP 303 + +GL F +L + G+Y + YW D ++F K + K P Sbjct: 248 CTILMAWASGLEQF----ETLGADICDGVYF-GAQYWHAIDAPFNKEFVKLVNDNLKIDP 302 Query: 304 SSLQAADYSAALQYLQAVKAAGTDDSDKVMAQLRKTKFNDVFQKNGYLRADGRVVHDMYL 363 + A Y L A+ A + D V+A + K+ + + D +V+ + YL Sbjct: 303 NYSLAGSYICTKIILDAIIKANSTDPKAVIAAMEGMKYEGLTGSEEIRKGDHQVIKNYYL 362 Query: 364 YQVKTPAESKYPWDYYKLVQT 384 + K A+ DY +V + Sbjct: 363 LKGKAKAKMANKNDYADIVSS 383 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.129 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,155,256 Number of extensions: 46663 Number of successful extensions: 221 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 210 Number of HSP's successfully gapped: 17 Length of query: 403 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 316 Effective length of database: 1,207,925 Effective search space: 381704300 Effective search space used: 381704300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)