BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0225.0025.p (203 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314069|ref|YP_003778161.1| amino acid ABC transporter perm... 325 2e-90 gi|300312953|ref|YP_003777045.1| amino acid ABC transporter perm... 124 4e-30 gi|300313933|ref|YP_003778025.1| branched-chain amino acid ABC t... 94 1e-20 gi|300311771|ref|YP_003775863.1| branched-chain amino acid ABC t... 93 2e-20 gi|300313285|ref|YP_003777377.1| branched-chain amino acid ABC t... 88 4e-19 >gi|300314069|ref|YP_003778161.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 326 Score = 325 bits (833), Expect = 2e-90, Method: Compositional matrix adjust. Identities = 159/174 (91%), Positives = 169/174 (97%) Query: 10 PFGLYPVFLMKMLCFALFACAFNLLIGYTGLLSFGHAAFFGGAGYVAGQAIKAWGVPTEI 69 PFG+YPVFLMKMLCFALFACAFNLLIGYTGLLSFGHAAF G AGY+ GQA+K WG+PTE+ Sbjct: 18 PFGIYPVFLMKMLCFALFACAFNLLIGYTGLLSFGHAAFLGWAGYMCGQALKVWGLPTEL 77 Query: 70 GLILGTLTGALIGLVMGLLAIRRQGIYFTMITLALAQMLFFVALQAPFTGGEDGLQGIPR 129 GLI+GTLTGALIGLVMGLLAIRRQGIYFTMITLALAQMLFFVALQAPFTGGEDGLQG+PR Sbjct: 78 GLIIGTLTGALIGLVMGLLAIRRQGIYFTMITLALAQMLFFVALQAPFTGGEDGLQGVPR 137 Query: 130 GKLLGFIDLSSDMTLYYVVLAIAIAGFALIVRTIHSPFGQVLKAIKENEPRAIS 183 GKLLGF++L SDMTLYYVVLAIA+AGFALIVRTIHSPFGQVLKAIKENEPRAIS Sbjct: 138 GKLLGFLNLESDMTLYYVVLAIAVAGFALIVRTIHSPFGQVLKAIKENEPRAIS 191 >gi|300312953|ref|YP_003777045.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 317 Score = 124 bits (312), Expect = 4e-30, Method: Compositional matrix adjust. Identities = 76/167 (45%), Positives = 107/167 (64%), Gaps = 6/167 (3%) Query: 18 LMKMLCFALFACAFNLLIGYTGLLSFGHAAFFGGAGYVAGQAIKAWGVPTEIGLI--LGT 75 L ++L FA+ A + N ++GY G++SFGHA FFG YV AI ++ T LI L Sbjct: 32 LTRILIFAMVAASLNFILGYGGMVSFGHAVFFGLGAYVT--AIASFHGVTSAWLIWLLAA 89 Query: 76 LTGALIGLVMGLLAIRRQGIYFTMITLALAQMLFFVALQAPFTGGEDGLQGIPRGKLLGF 135 L AL+GLV+G++A+R + +YF MIT+ALAQ+ ++ + + GG+DGLQ R LLGF Sbjct: 90 LVTALVGLVIGVIALRTRAVYFIMITMALAQLFYYFFVGFRYYGGDDGLQVTAR-PLLGF 148 Query: 136 -IDLSSDMTLYYVVLAIAIAGFALIVRTIHSPFGQVLKAIKENEPRA 181 +D+S D Y+VVLA A F L+ R + S FG L AI++NE RA Sbjct: 149 GLDMSGDNAFYWVVLAWLAASFILLQRLLASRFGLALNAIRQNERRA 195 >gi|300313933|ref|YP_003778025.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 334 Score = 93.6 bits (231), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 70/167 (41%), Positives = 96/167 (57%), Gaps = 5/167 (2%) Query: 20 KMLCFALFACAFNLLIGYTGLLSFGHAAFFGGAGYVAGQAIKAWGVPTEIGLILGTLTGA 79 ++L FAL A NLL+GYTGL+SFG FFG Y AG + + L L L GA Sbjct: 32 EVLVFALAALGCNLLLGYTGLMSFGQGIFFGVGSYTAGVVLLQLKLALLPALALAALGGA 91 Query: 80 LIGLVMGLLAIRRQGIYFTMITLALAQMLFFVALQ-APFTGGEDGLQGIPRGKL----LG 134 L+ L++G +IR++G YF M+TLA AQM +F+A TGG++GL IPR L L Sbjct: 92 LVALLVGWFSIRQRGTYFVMLTLAFAQMFYFLAYTMKDVTGGDNGLLDIPRPPLALFGLT 151 Query: 135 FIDLSSDMTLYYVVLAIAIAGFALIVRTIHSPFGQVLKAIKENEPRA 181 + +S Y VV + + F L+ R + S G+ L A++ENE RA Sbjct: 152 LLPTTSSWQYYTVVAILFVLVFWLLQRVVDSVLGKTLLAVRENEARA 198 >gi|300311771|ref|YP_003775863.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 628 Score = 92.8 bits (229), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 59/172 (34%), Positives = 90/172 (52%), Gaps = 6/172 (3%) Query: 11 FGLYPVFLMKMLCFALFACAFNLLIGYTGLLSFGHAAFFGGAGYVAGQAIKAWGVPTEIG 70 F PV ++++L LFA + + L+G G++SFGHAA+FG Y A P + Sbjct: 344 FPYLPVLMVEILVAVLFAASLHFLMGPGGMVSFGHAAYFGLGAYAAALVTLRLQWPMPLA 403 Query: 71 LILGTLTGALIGLVMGLLAIRRQGIYFTMITLALAQMLFFVALQ-APFTGGEDGLQGIPR 129 ++ G T L L+ G +R G+Y M+TLA AQ+++ Q TGG +GL G+ Sbjct: 404 MLAGAGTAMLAALLFGWFCVRLSGVYLAMLTLAFAQIVWAAIYQWDDLTGGSNGLVGLRT 463 Query: 130 GKLLGFIDLSSDMTLYYVVLAIAIAGFALIVRTIHSPFGQVLKAIKENEPRA 181 L+S + YY+ L + G L+ R IH+PFG L+A ++ RA Sbjct: 464 AA-----ALASPLAYYYLALLCSALGVYLLRRVIHAPFGLALRAARDASGRA 510 >gi|300313285|ref|YP_003777377.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 338 Score = 88.2 bits (217), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 62/170 (36%), Positives = 96/170 (56%), Gaps = 4/170 (2%) Query: 14 YPVFLMKMLCFALFACAFNLLIGYTGLLSFGHAAFFGGAGYVAG-QAIKAWGVPTEIGLI 72 Y V + +++ LFA + +L++GY G++S GHAAFFG Y AG A+ WG P GL+ Sbjct: 47 YLVLISQIMIVGLFAVSLDLILGYAGIVSLGHAAFFGLGAYTAGLLAVHGWGEPIT-GLL 105 Query: 73 LGTLTGALIGLVMGLLAIRRQGIYFTMITLALAQMLFFVALQAPF-TGGEDGLQGIPRGK 131 L + A+ G ++ L +R + M+TL + MLF A +A F TGG DGL G+ GK Sbjct: 106 LAAVVAAIAGFLVSFLVVRGADLTRLMVTLGIGLMLFEAANKAAFITGGVDGLSGMVMGK 165 Query: 132 LLGFIDLSSDMTLYY-VVLAIAIAGFALIVRTIHSPFGQVLKAIKENEPR 180 + G + + + Y A+ + F ++ R ++SPFG L+ I+E R Sbjct: 166 IFGVFEFDLNGRVAYGYSFAVLLLLFVILRRLVNSPFGLGLRGIREGGRR 215 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.331 0.148 0.438 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 470,143 Number of extensions: 15706 Number of successful extensions: 99 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 86 Number of HSP's successfully gapped: 14 Length of query: 203 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 122 Effective length of database: 1,236,335 Effective search space: 150832870 Effective search space used: 150832870 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 128 (53.9 bits)