BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0225.0024.p (122 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314069|ref|YP_003778161.1| amino acid ABC transporter perm... 201 1e-53 >gi|300314069|ref|YP_003778161.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 326 Score = 201 bits (512), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 105/122 (86%), Positives = 111/122 (90%) Query: 1 VLSAALTGLAGSTKALVLGFETLTDVHWSMSGLVVLMTLVGGLGTIVGPIVGAVIIIMLE 60 VLSA L+GLAG+TK LVLGFETLTDVHWSMSGLVVLMTLVGGLGTI+GPIVGAVIIIMLE Sbjct: 205 VLSAGLSGLAGATKTLVLGFETLTDVHWSMSGLVVLMTLVGGLGTILGPIVGAVIIIMLE 264 Query: 61 NKLGDIGNWLAGVTHIEWFNTLGDSVTIVTGFIFIVCVLAFRRGIVGEILHLFGKRSPTA 120 NKLGDIG WLA VTHI+WFNTLGDSVTIVTGFIFIVCVLAFR+GIVGEIL L K + Sbjct: 265 NKLGDIGAWLASVTHIDWFNTLGDSVTIVTGFIFIVCVLAFRKGIVGEILALTRKGQASG 324 Query: 121 SH 122 SH Sbjct: 325 SH 326 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.143 0.437 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 294,009 Number of extensions: 7975 Number of successful extensions: 33 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 33 Number of HSP's successfully gapped: 3 Length of query: 122 Length of database: 1,619,870 Length adjustment: 74 Effective length of query: 48 Effective length of database: 1,269,480 Effective search space: 60935040 Effective search space used: 60935040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 125 (52.8 bits)