BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0225.0019.p (244 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310396|ref|YP_003774488.1| sugar ABC transporter ATPase [H... 291 4e-80 gi|300310382|ref|YP_003774474.1| sugar ABC transporter ATPase [H... 198 4e-52 gi|300310063|ref|YP_003774155.1| sugar ABC transporter ATPase [H... 197 7e-52 gi|300310371|ref|YP_003774463.1| sugar ABC transporter ATPase [H... 169 2e-43 gi|300313728|ref|YP_003777820.1| sugar ABC transporter ATPase [H... 169 3e-43 >gi|300310396|ref|YP_003774488.1| sugar ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 291 bits (745), Expect = 4e-80, Method: Compositional matrix adjust. Identities = 142/238 (59%), Positives = 177/238 (74%) Query: 1 MDVRAIQLRRDGPVNRFQLREGEILGFAGLVGSGRTELALGLIGADPCHRKDVSVHGVTM 60 ++V A+QLRR PV++FQLR GEILGFAGLVGSGRTE L ++GA +S+HGV + Sbjct: 263 LEVHALQLRRQAPVSQFQLRRGEILGFAGLVGSGRTETVLAMLGAHAALSCKLSMHGVPL 322 Query: 61 ALHDPAEALVAGVGLLPENRKTEGLILDFSIKDNISLNNLQKYCSALPFIRRGAEREDAQ 120 DPA+AL AG+GLLPE+RK +GLI FSI N+SLNN KY F+ R E++ + Sbjct: 323 RFADPAQALQAGIGLLPESRKEQGLITSFSILHNVSLNNYGKYRLGGLFLDRRREQQATE 382 Query: 121 CMSRDIDIKAPDVFARVGNLSGGNQQKVVIARWLNHHCKILIFDEPTRGIDVGAKAEIYR 180 + + +KAP RV LSGGNQQKVVIARW+NH K+LIFDEPTRGIDVGAK+EIY+ Sbjct: 383 AAMQRVRVKAPGAQVRVDTLSGGNQQKVVIARWINHAMKVLIFDEPTRGIDVGAKSEIYQ 442 Query: 181 LMRRLTVQGYAVIMISSELPEIVGMCDRVAVFRQGAIVTILEGDSIQSNEVMRHATTG 238 LMR T QGY+++MISSELPE+VGM DRV VFR G IV LEG+++ + E+M HATTG Sbjct: 443 LMREFTAQGYSILMISSELPEVVGMADRVCVFRGGGIVATLEGEAVNAEEIMTHATTG 500 Score = 73.9 bits (180), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 63/204 (30%), Positives = 95/204 (46%), Gaps = 8/204 (3%) Query: 17 FQLREGEILGFAGLVGSGRTELALGLIGADPCHRKDVSVHGVTMALHDPAEALVAGVGLL 76 ++R GE+ G G+G++ L L G ++ + G A+ GVG++ Sbjct: 26 LEVRAGEVHALLGENGAGKSTLMKILCGIVRPDAGEIRIDGQPCRFDSYRAAIAGGVGVV 85 Query: 77 PENRKTEGLILDFSIKDNISLNNLQKYCSALPFIRRGAEREDAQCMSRDIDIKAP-DVFA 135 + LI DN+ L ++ S +++R A R AQ + + + P DV Sbjct: 86 FQEFS---LIPYLDAVDNMFLA--RELRSRWGWLQRAAMRRRAQEIIGQLGVAIPLDV-- 138 Query: 136 RVGNLSGGNQQKVVIARWLNHHCKILIFDEPTRGIDVGAKAEIYRLMRRLTVQGYAVIMI 195 V LS QQ V IA+ L +IL+ DEPT + ++ +MR L QG A+I I Sbjct: 139 PVCKLSVAQQQFVEIAKALALDARILVLDEPTATLTPAEVEHLFAVMRSLRAQGVAIIFI 198 Query: 196 SSELPEIVGMCDRVAVFRQGAIVT 219 S L EI +CDR+ V R GA V Sbjct: 199 SHHLEEIFEICDRITVLRDGAYVA 222 >gi|300310382|ref|YP_003774474.1| sugar ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 520 Score = 198 bits (503), Expect = 4e-52, Method: Compositional matrix adjust. Identities = 104/220 (47%), Positives = 145/220 (65%), Gaps = 1/220 (0%) Query: 17 FQLREGEILGFAGLVGSGRTELALGLIGADPCHRKDVSVHGVTMALHDPAEALVAGVGLL 76 F LR+GEILGFAGL+G+GRTE+A + GADP ++ +HG + PA+A+ G+G L Sbjct: 294 FTLRKGEILGFAGLMGAGRTEVARAIFGADPLEAGEIIIHGGKAVIKSPADAVAHGIGYL 353 Query: 77 PENRKTEGLILDFSIKDNISLNNLQKYCSALPFIRRGAEREDAQCMSRDIDIKAPDVFAR 136 E+RK GL + ++ NI+L+++ ++ + + F+ + A RE AQ R + IK P V + Sbjct: 354 SEDRKHFGLAVGMDVQANIALSSMGRF-TRVGFMDQRAIREAAQMYVRQLAIKTPSVEQQ 412 Query: 137 VGNLSGGNQQKVVIARWLNHHCKILIFDEPTRGIDVGAKAEIYRLMRRLTVQGYAVIMIS 196 LSGGNQQK+VIA+WL C IL FDEPTRGIDVGAK+EIY+L+ L QG A++MIS Sbjct: 413 ARLLSGGNQQKIVIAKWLLRDCDILFFDEPTRGIDVGAKSEIYKLLDALAEQGKAIVMIS 472 Query: 197 SELPEIVGMCDRVAVFRQGAIVTILEGDSIQSNEVMRHAT 236 SELPE++ M RV V +G I L ++M+ AT Sbjct: 473 SELPEVLRMSHRVLVMCEGRITGELARADATQEKIMQLAT 512 Score = 81.6 bits (200), Expect = 5e-17, Method: Compositional matrix adjust. Identities = 58/209 (27%), Positives = 100/209 (47%), Gaps = 11/209 (5%) Query: 16 RFQLREGEILGFAGLVGSGRTELALGLIGADPCHRKDVSVHGVTMALHDPAEALVAGVGL 75 +F+L GE+ G G+G++ L L G D+ + G + + +P +A G+G+ Sbjct: 42 QFELAAGEVHALMGENGAGKSTLMKILSGVYQRDSGDILLDGKPVEITEPRQAQALGIGI 101 Query: 76 LPENRKTEGLILDFSIKDNISLNNLQKYCSALPFIRRGAEREDAQCMSR---DIDIKAPD 132 + + L+ S NI + + L R+ A +R D+D P Sbjct: 102 IHQELN---LMNHLSAAQNIFIGREPRKAMGLFIDEDELNRQAAAIFARMRLDMDPSTP- 157 Query: 133 VFARVGNLSGGNQQKVVIARWLNHHCKILIFDEPTRGIDVGAKAEIYRLMRRLTVQGYAV 192 VG L+ QQ V IA+ L+ ++LI DEPT ++ AE++R++R L QG + Sbjct: 158 ----VGELTVARQQMVEIAKALSFDSRVLIMDEPTAALNNAEIAELFRIIRDLQAQGVGI 213 Query: 193 IMISSELPEIVGMCDRVAVFRQGAIVTIL 221 + IS ++ E+ + DRV+V R G + + Sbjct: 214 VYISHKMDELRQIADRVSVMRDGKYIATV 242 >gi|300310063|ref|YP_003774155.1| sugar ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 521 Score = 197 bits (501), Expect = 7e-52, Method: Compositional matrix adjust. Identities = 107/236 (45%), Positives = 151/236 (63%), Gaps = 2/236 (0%) Query: 1 MDVRAIQLRRDGPVNRFQLREGEILGFAGLVGSGRTELALGLIGADPCHRKDVSVHGVTM 60 M VRA+ + P + F +R GE+LGFAGLVG+GRTELA L GADP D+ + G + Sbjct: 265 MQVRALAGGKIRPAS-FDVRAGEVLGFAGLVGAGRTELARLLFGADPRSGGDILLEGRPV 323 Query: 61 ALHDPAEALVAGVGLLPENRKTEGLILDFSIKDNISLNNLQKYCSALPFIRRGAEREDAQ 120 + P A+ AG+ +PE+RK +GL L ++ N ++N ++ L +R + A+ Sbjct: 324 HIDQPRAAMRAGIAYVPEDRKGQGLFLQMAVAANATMNVASRHTR-LGLVRSRSLGGVAR 382 Query: 121 CMSRDIDIKAPDVFARVGNLSGGNQQKVVIARWLNHHCKILIFDEPTRGIDVGAKAEIYR 180 + +++K VG LSGGNQQKV++ARWL K+LI DEPTRG+D+ AK+EIY+ Sbjct: 383 AAIQRLNVKVAHPETPVGKLSGGNQQKVLLARWLEIAPKVLILDEPTRGVDIYAKSEIYQ 442 Query: 181 LMRRLTVQGYAVIMISSELPEIVGMCDRVAVFRQGAIVTILEGDSIQSNEVMRHAT 236 L+ RL QG AV++ISSELPE++G+CDRV V R+G I L G +I +MR AT Sbjct: 443 LVHRLASQGVAVVVISSELPEVIGICDRVLVMREGMITGELAGAAITQENIMRLAT 498 Score = 87.0 bits (214), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 67/229 (29%), Positives = 109/229 (47%), Gaps = 10/229 (4%) Query: 17 FQLREGEILGFAGLVGSGRTELALGLIGADPCHRKDVSVHGVTMALHDPAEALVAGVGLL 76 +R GEI G G+G++ L L G + ++ + G +AL DP + AG+ L+ Sbjct: 27 LTIRPGEIHALMGENGAGKSTLMKVLSGVHAPDQGEILLDGRPVALRDPGASRAAGINLI 86 Query: 77 PENRKTEGLILDFSIKDNISLNNLQKYCSALPFIRRGAEREDAQCMSRDID--IKAPDVF 134 + + + S+ N+ + + + + L I A R + R + A D+ Sbjct: 87 YQEL---AVAPNISVAANVFMGS--ELRTRLGLIDHAAMRSRTDAVLRQLGAGFGASDL- 140 Query: 135 ARVGNLSGGNQQKVVIARWLNHHCKILIFDEPTRGIDVGAKAEIYRLMRRLTVQGYAVIM 194 G LS QQ+V IAR L H +I+I DEPT + +++ ++RRL +G A+I Sbjct: 141 --AGRLSIAEQQQVEIARALVHRSRIVIMDEPTAALSERETEQLFNVVRRLRDEGLAIIY 198 Query: 195 ISSELPEIVGMCDRVAVFRQGAIVTILEGDSIQSNEVMRHATTGSRLEF 243 IS + E+ + DRV V R G+ V L D I S +++ S EF Sbjct: 199 ISHRMAEVYALADRVTVLRDGSFVGELVRDEIDSERIVQMMVGRSLSEF 247 >gi|300310371|ref|YP_003774463.1| sugar ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 518 Score = 169 bits (427), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 95/230 (41%), Positives = 136/230 (59%), Gaps = 7/230 (3%) Query: 17 FQLREGEILGFAGLVGSGRTELALGLIGADPCHRKDVS----VHGVTMALHDPAEALVAG 72 +R GEI+G AGL+G+GRTELA + G + K ++ +HG + L +A+ G Sbjct: 284 MSVRAGEIVGIAGLMGAGRTELAKSIFGR--AYGKKITGQAFLHGKEVDLSTIEKAIAKG 341 Query: 73 VGLLPENRKTEGLILDFSIKDNISLNNLQKYCSALPFIRRGAEREDAQCMSRDIDIKAPD 132 + + E+RK +GL+L+ IK NISL NL S I E + A + + I+ Sbjct: 342 IAYVTEDRKGDGLVLEEDIKKNISLANLGG-VSERTVIDEAREYKIAADFKQQMRIRCSS 400 Query: 133 VFARVGNLSGGNQQKVVIARWLNHHCKILIFDEPTRGIDVGAKAEIYRLMRRLTVQGYAV 192 V +V NLSGGNQQKVV+++WL ++LI DEPTRGIDVGAK EIY ++ +L +G + Sbjct: 401 VLQKVVNLSGGNQQKVVLSKWLFSQPEVLILDEPTRGIDVGAKFEIYNIISKLAAEGKCI 460 Query: 193 IMISSELPEIVGMCDRVAVFRQGAIVTILEGDSIQSNEVMRHATTGSRLE 242 IMISSE+PE++GMCDR+ V +G V L +MR R++ Sbjct: 461 IMISSEMPELLGMCDRIYVMNEGQFVGHLPKAEATQENIMRAIMRNKRID 510 Score = 64.7 bits (156), Expect = 7e-12, Method: Compositional matrix adjust. Identities = 59/223 (26%), Positives = 102/223 (45%), Gaps = 17/223 (7%) Query: 19 LREGEILGFAGLVGSGRTELALGLIGADP--CHRKDVSVHGVTMALHDPAEALVAGVGLL 76 +REGEI G G+G++ L L G P + D+ G A D ++ G+ ++ Sbjct: 27 VREGEIHAIVGENGAGKSTLMKVLSGVYPHGSYSGDIVYKGEVRAFKDIRDSEHLGIIII 86 Query: 77 PENRKTEGLILDFSIKDNISLNNLQKYCSALPFIRRGAEREDAQCMSRDIDIKA---PDV 133 + L+ S+ +N+ L N Q + + E + ++++ K Sbjct: 87 HQEL---ALVPLLSVMENLFLGNEQARGGVIDW-------EQSYVRAKELLAKVGLKESP 136 Query: 134 FARVGNLSGGNQQKVVIARWLNHHCKILIFDEPTRGIDVGAKAEIYRLMRRLTVQGYAVI 193 +VG+L G QQ + IA+ L+ K+LI DEPT ++ + L+ L QG A I Sbjct: 137 LTQVGDLGVGKQQLIEIAKALSKEVKLLILDEPTASLNESDSDALLELLLELKRQGIASI 196 Query: 194 MISSELPEIVGMCDRVAVFRQGAIVTILE--GDSIQSNEVMRH 234 +IS +L EI + D + V R G V + + I + +++H Sbjct: 197 LISHKLNEISKVADSITVLRDGTTVDTFDCRAEPISEDRIIQH 239 >gi|300313728|ref|YP_003777820.1| sugar ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 546 Score = 169 bits (427), Expect = 3e-43, Method: Compositional matrix adjust. Identities = 89/207 (42%), Positives = 128/207 (61%), Gaps = 5/207 (2%) Query: 17 FQLREGEILGFAGLVGSGRTELALGLIGADPCHRKDVSVHGVTMALHDPAEALVAGVGLL 76 F +R GEILGF GLVG+GR+E+ L G P + V + G + L PA+A+ G+ + Sbjct: 309 FAVRPGEILGFYGLVGAGRSEVMHALFGLSPEAQGAVWIDGREVKLCSPAQAIAHGLAYV 368 Query: 77 PENRKTEGLILDFSIKDNISLNNLQKYCSALPFIRRGAERED--AQCMSRDIDIKAPDVF 134 PE+R+ +G +L I NI+L L F+RR RE A+ + +++KA Sbjct: 369 PEDRQRQGALLSLPIFQNITLPVLPGIGF---FLRRHRRREIDIARRLCEQLELKASHFH 425 Query: 135 ARVGNLSGGNQQKVVIARWLNHHCKILIFDEPTRGIDVGAKAEIYRLMRRLTVQGYAVIM 194 V LSGGNQQKVV+A+WL ++LI DEPT+GID+G+KA ++R + L QG AVI+ Sbjct: 426 QHVAQLSGGNQQKVVLAKWLATQPRVLILDEPTKGIDIGSKAAVHRFIGELVAQGLAVIL 485 Query: 195 ISSELPEIVGMCDRVAVFRQGAIVTIL 221 +SSELPE++GM DR+ V QG + + Sbjct: 486 VSSELPEVMGMSDRIVVMHQGRVQQVF 512 Score = 72.0 bits (175), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 54/203 (26%), Positives = 94/203 (46%), Gaps = 5/203 (2%) Query: 17 FQLREGEILGFAGLVGSGRTELALGLIGADPCHRKDVSVHGVTMALHDPAEALVAGVGLL 76 F +R GE++ G G+G++ L L G + + G + +A+ G+ + Sbjct: 56 FTVRPGEVMALLGENGAGKSTLVKILTGIHQPDEGSIHLGGREVRFASAQDAMRGGITAV 115 Query: 77 PENRKTEGLILDFSIKDNISLNNLQKYCSALPFIRRGAEREDAQCMSRDIDIKAPDVFAR 136 + + + S+ +NI + Q C I ++A+ + +++ P V AR Sbjct: 116 HQETV---MFEELSVAENIWIGR-QPLCGTPRRIDWRRMEDEARALFARLEVDLP-VRAR 170 Query: 137 VGNLSGGNQQKVVIARWLNHHCKILIFDEPTRGIDVGAKAEIYRLMRRLTVQGYAVIMIS 196 V +LS + V IAR L+ +++I DEPT + E+YR++ +L G AVI IS Sbjct: 171 VKDLSVAQRHFVEIARALSQQAQVVIMDEPTAALSHHEIGELYRIIGQLRRAGTAVIFIS 230 Query: 197 SELPEIVGMCDRVAVFRQGAIVT 219 + EI + DR V R G + Sbjct: 231 HKFDEIYAVADRYTVLRDGRFIA 253 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.140 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 769,366 Number of extensions: 32306 Number of successful extensions: 320 Number of sequences better than 1.0e-08: 62 Number of HSP's gapped: 290 Number of HSP's successfully gapped: 73 Length of query: 244 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 161 Effective length of database: 1,226,865 Effective search space: 197525265 Effective search space used: 197525265 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 129 (54.3 bits)