BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0225.0016.p
         (223 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspi...    65   5e-12
gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldeh...    65   6e-12
gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herb...    61   8e-11

>gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 341

 Score = 65.1 bits (157), Expect = 5e-12,   Method: Compositional matrix adjust.
 Identities = 57/165 (34%), Positives = 81/165 (49%), Gaps = 16/165 (9%)

Query: 14  PPVVAGHEFFGYVAALGEGAAEHFGVQQGDRVIAEQI-VPCEACLFCKTGKYWMCEVHNI 72
           PP + GHE  GYVAA+G G      V++GDRV    +   C  C  C+TG   +C     
Sbjct: 59  PPFIPGHEGTGYVAAVGAGVKH---VKEGDRVGVPWLHTACGCCSPCRTGWETLCAEQQN 115

Query: 73  FGFQREVADGGMAEYMRLPSNAVVHKIPMDMPLEDAAIIEPLSCAIHTVNRG----DIQL 128
            G+     +G  AEY  L     V  +P ++    AA   P+ CA  TV +G    +++ 
Sbjct: 116 TGYS---VNGSFAEY-GLADPKFVGHLPDNLDFGPAA---PVLCAGVTVYKGLKETEVRP 168

Query: 129 DDVVVIAGAGPLGLMMTQVAKLKTPKKLVVVDLVPERLALARXCG 173
            + VVI+G G LG M  Q AK      +V  D+  ++LALA+  G
Sbjct: 169 GEWVVISGIGGLGHMAVQYAK-AMGMHVVAADIHEDKLALAKKLG 212


>gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldehyde
           dehydrogenase [Herbaspirillum seropedicae SmR1]
          Length = 386

 Score = 64.7 bits (156), Expect = 6e-12,   Method: Compositional matrix adjust.
 Identities = 52/186 (27%), Positives = 78/186 (41%), Gaps = 26/186 (13%)

Query: 7   GQDSWVKPPVVAGHEFFGYVAALGEGAAEHFGVQQGDRVIAEQIVPCEACLFCKTGKYWM 66
           G+    K   + GHEF G V   G        +++GDRV+   ++ C  C FC    Y  
Sbjct: 47  GKIPATKAGDILGHEFMGVVVEAGPAIKR---LKKGDRVVVPFVIACGQCFFCDQHLYAA 103

Query: 67  CEVHN-----------------IFGFQREVAD--GGMAEYMRLPSNAVVHKIPMDMPLED 107
           CE  N                 +FG+        GG AEY+R+P  A V  I +D  L D
Sbjct: 104 CETTNPDRGALMNAKGIRSGAAMFGYSHLYGGMPGGQAEYVRVP-KADVGPIRIDSALAD 162

Query: 108 AAII---EPLSCAIHTVNRGDIQLDDVVVIAGAGPLGLMMTQVAKLKTPKKLVVVDLVPE 164
             ++   + L      V    ++    V I GAGP+G M    A++   +++ +VD    
Sbjct: 163 EQVLFLSDILPTGYQAVKNAGVREGSSVAIYGAGPVGQMAAASARMLGAERIFMVDHHAY 222

Query: 165 RLALAR 170
           RL  A+
Sbjct: 223 RLRFAQ 228


>gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 345

 Score = 60.8 bits (146), Expect = 8e-11,   Method: Compositional matrix adjust.
 Identities = 46/166 (27%), Positives = 73/166 (43%), Gaps = 4/166 (2%)

Query: 12  VKPPVVAGHEFFGYVAALGEGAAEHFGVQQGDRVIAEQIVPCEACLFCKTGKYWMCEVHN 71
           +K P+V GHE  G +A +G    +   +  G R+      PC AC +C+ GK   C    
Sbjct: 55  IKEPMVLGHEVSGQIAEVGTAVTD---IAPGTRIAISPSRPCGACKYCQEGKQNHCLDMR 111

Query: 72  IFGF-QREVADGGMAEYMRLPSNAVVHKIPMDMPLEDAAIIEPLSCAIHTVNRGDIQLDD 130
            +G   R     G      +   +  H +   +   +AA+ EPLS A+H V R    +  
Sbjct: 112 FYGSAMRTPHVQGAFRQEIIIERSQAHVVADHVSDGEAAMAEPLSVALHAVRRAGPLVGK 171

Query: 131 VVVIAGAGPLGLMMTQVAKLKTPKKLVVVDLVPERLALARXCGHRS 176
            V++ G GP+G ++   A+     ++VV D+    L  A   G  S
Sbjct: 172 RVLVTGCGPIGALVVVAARRAGALEVVVTDVASMPLQAALQVGADS 217


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.325    0.142    0.458 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 757,559
Number of extensions: 31799
Number of successful extensions: 130
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 127
Number of HSP's successfully gapped: 3
Length of query: 223
Length of database: 1,619,870
Length adjustment: 82
Effective length of query: 141
Effective length of database: 1,231,600
Effective search space: 173655600
Effective search space used: 173655600
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 129 (54.3 bits)