BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0225.0013.p (398 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310425|ref|YP_003774517.1| gluconokinase [Herbaspirillum s... 159 3e-40 gi|300309787|ref|YP_003773879.1| sugar (pentulose and hexulose) ... 122 7e-29 gi|300312696|ref|YP_003776788.1| D-xylulose kinase [Herbaspirill... 113 3e-26 gi|300310306|ref|YP_003774398.1| glycerol kinase [Herbaspirillum... 100 3e-22 gi|300310399|ref|YP_003774491.1| D-ribulose/ribitol kinase [Herb... 83 5e-17 >gi|300310425|ref|YP_003774517.1| gluconokinase [Herbaspirillum seropedicae SmR1] Length = 520 Score = 159 bits (402), Expect = 3e-40, Method: Compositional matrix adjust. Identities = 116/378 (30%), Positives = 187/378 (49%), Gaps = 25/378 (6%) Query: 14 TGNGVDSYYGYTKMLWLKNRRPEVWAKTRYFLPPNSYVNYLLTGELAVDHSSAGNIGGVY 73 TG V K++W+++ +P+V+ + F+ Y+ Y L G+ VDHS A + G++ Sbjct: 131 TGTPVHPMSPLCKLMWMRHEKPDVFHRAARFVGIKEYLLYQLFGQWVVDHSIA-SATGMF 189 Query: 74 DITQRCWSTEAMAMLGIPPMLMPERLIASSDVVGPLLPSQ-AMALGLAAGTPVIAGGVDA 132 ++ + W A+A+LG+ P +P + + + P L S+ A LGL+ TP I G D Sbjct: 190 NLRELAWDQGALALLGVRPDQLPTPVPTTHRL--PALSSEMAQRLGLSVDTPFIIGANDG 247 Query: 133 AMATFAAGVTKAGNHVAMIGTS-MCWGYINQQVDAGHGLISMPHVYNGLQDLYIFGGAIT 191 ++ + G+ IGTS I++ G + Y + ++ GG + Sbjct: 248 VLSNLGVNAIEIGHVAVTIGTSGAMRTVIDEPRTDPQGRL---FCYALTEKHWVVGGPVN 304 Query: 192 AGAAV-SWFLEQFCQAEMTEALRSDVDPHFLLERDAAAIAPGAEGIVFLPYLMGERGPIW 250 G + W ++ AE A VDP+ L R A + PGAEG++F P++ GER P+W Sbjct: 305 NGGNIFRWVRDELATAEAAAAREEGVDPYEALTRIAEKVRPGAEGLLFHPFMAGERAPLW 364 Query: 251 DAKASGAFVGLSLYHRRAHLYRAVLEGVAFALKHNIDAGACGAQFLDE------RLVVVG 304 +A G+F GL+L+H + H+ RA LEGV F L + A L+E R++ G Sbjct: 365 NADLRGSFFGLALHHGKHHMIRAALEGVIFNLYSILPA-------LEELVGPTKRMMATG 417 Query: 305 GASHSNLWMQIIADVTGYPVYTIDDNVEAALGAALLAAFGVGLIDTSQVEKGWARLRLRT 364 G + S LW Q++AD+ V + + LGAA+L A+ +GL+ + V G Sbjct: 418 GFARSALWRQMMADIFNREVVVPESVESSCLGAAVLGAWALGLVPSLSVISGMVGSTNHH 477 Query: 365 TPD---IAASRRYQHIFA 379 P+ +A R Q IFA Sbjct: 478 APEAEAVAVYGRLQPIFA 495 >gi|300309787|ref|YP_003773879.1| sugar (pentulose and hexulose) kinase [Herbaspirillum seropedicae SmR1] Length = 490 Score = 122 bits (305), Expect = 7e-29, Method: Compositional matrix adjust. Identities = 118/379 (31%), Positives = 175/379 (46%), Gaps = 18/379 (4%) Query: 9 RLAVLTGNGVDSYYGYTKMLWLKNRRPEVWAKTRYFLPPNSYVNYLLTGELAVDHSSAGN 68 RL + GN + K+LW+ P+++A+ L P ++ +TG D S A Sbjct: 117 RLHGIAGNLAMPGFTAPKLLWVARHEPQLFARIATVLLPKDWLRLKMTGRKVSDPSDAA- 175 Query: 69 IGGVY-DITQRCWSTEAMAMLGIPPMLMPERLIASSDVVGPLLPSQAMALGLAAGTPVIA 127 G ++ D+ R WS E +A G+ MP L+ S V G LLP A A GL + V Sbjct: 176 -GTLWLDVEGRNWSDELLAASGMRRDQMPA-LVDGSAVSGSLLPEVAQAWGLRSDVIVAG 233 Query: 128 GGVDAAMATFAAGVTKAGNHVAMIGTSMCWGYINQQVDAGHGLISMPHVYNGLQDLYIFG 187 G D A + G K G+ +GTS +N + G + Q + Sbjct: 234 GAGDGAASAVGIGAVKPGDGFLSLGTSGVLFVVNDRFRPNPGRAIHAFCHTLPQRWHQMS 293 Query: 188 GAITAGAAVSWFLEQFCQAEMTEALRSDVDPHFLLERDAAAIAPGAEGIVFLPYLMGERG 247 ++A + + WF + C + ++L ++++ L+ A AP +FLPYL GER Sbjct: 294 VMLSAASCLRWFC-RLCSVD-EKSLLAEIEQ---LDEQACNNAP-----LFLPYLSGERT 343 Query: 248 PIWDAKASGAFVGLSLYHRRAHLYRAVLEGVAFALKHNIDAGACGAQFLDERLVVVGGAS 307 P D A+G F GL+ H+RA L AVLEGVAF + +DA + E L +VGG + Sbjct: 344 PHNDPYATGVFHGLTPEHQRAALGYAVLEGVAFGMVDGLDALRAAGTDVAE-LSLVGGGA 402 Query: 308 HSNLWMQIIADVTGYPVYT-IDDNVEAALGAALLAAFGVGLIDTSQVEKGWARLRLRTTP 366 S W Q++AD + T + ALGAA LA G D ++V + A+ L P Sbjct: 403 RSAYWAQLLADALQVRIVTHVGSEAGGALGAARLAWLADG-GDEAEVCRKPAQQAL-YQP 460 Query: 367 DIAASRRYQHIFAQYRGLY 385 D A Q +YR +Y Sbjct: 461 DPARHAALQARLQRYRAIY 479 >gi|300312696|ref|YP_003776788.1| D-xylulose kinase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 113 bits (282), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 125/398 (31%), Positives = 169/398 (42%), Gaps = 37/398 (9%) Query: 5 VDLERLA----VLTGNGVDSYYGYTKMLWLKNRRPEVWAKTRYFLPPNSYVNYLLTGELA 60 V+LE L +TGN + K+LWL P V+ L P ++ + LTGE Sbjct: 109 VELEALVPDAVAITGNRAMPGFTAPKLLWLSKYEPAVYRAIDKVLLPKDFLGWKLTGEFV 168 Query: 61 VDHSSAGNIGGVY-DITQRCWSTEAMAMLGIPPMLMPERLIASSDVVGPLLPSQAMALGL 119 + S A G ++ D+ +R WS + G+ MP RL+ S V G L G+ Sbjct: 169 SEMSDAA--GTLWLDVARRDWSERMLGATGLDRSHMP-RLVEGSAVAGQLRDELRREWGI 225 Query: 120 AAGTPVIAGGV-DAAMATFAAGVTKAGNHVAMIGTS--MCWGYINQQVDAGHGLISMPHV 176 + G V+AGG D A + GV +AG+ +G+S + + +A G+ + H Sbjct: 226 S-GPVVVAGGAGDNAASAVGIGVIRAGDAFLSLGSSGVLFAATAHHAPNAQQGVHAFCHC 284 Query: 177 YNGLQDLYIFGGAITAGAAVSWFLEQFCQAEMTEALRSDVDPHFLLERDAAAIAPGAEGI 236 G + ++A A++ W A L S + L A AP Sbjct: 285 LPG--QWHQMSVILSAAASLHWL--SGVTARPVAELVSGAE---RLSAGQQAQAP----- 332 Query: 237 VFLPYLMGERGPIWDAKASGAFVGLSLYHRRAHLYRAVLEGVAFALKHNIDAGACGAQFL 296 +FLPYL GER P DA A G G++ H AHL AV+EGVAFA+ A L Sbjct: 333 LFLPYLNGERTPHNDAAAKGVLFGMTPAHESAHLAYAVMEGVAFAMADGYAALQAAGTTL 392 Query: 297 DERLVVVGGASHSNLWMQIIADVTGYPVYT-IDDNVEAALGAALLAAFGVGLIDTSQV-- 353 E VGG S S W + A G P+ V AALGAA L D + V Sbjct: 393 -ESAAFVGGGSRSAFWGVLCATALGIPLRRHAGAEVGAALGAARLGRLARTGEDAASVCI 451 Query: 354 ---EKGWARLRLRTTPDIAASRRYQHIFAQYRGLYPSL 388 W R PD A + Q AQYR LY SL Sbjct: 452 PPEVLEWYR------PDAARQAQLQRRLAQYRRLYRSL 483 >gi|300310306|ref|YP_003774398.1| glycerol kinase [Herbaspirillum seropedicae SmR1] Length = 495 Score = 100 bits (248), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 110/382 (28%), Positives = 164/382 (42%), Gaps = 29/382 (7%) Query: 8 ERLAVLTGNGVDSYYGYTKMLWLKNRRPEVWAKTR----YFLPPNSYVNYLLTG-ELAVD 62 E + TG +D Y+ TK+ W+ + + A+ F +S++ + LTG L V Sbjct: 120 EAIQKKTGLVLDPYFSGTKLRWILDHVDGLRARAERGELLFGTVDSWLVWNLTGGRLHVS 179 Query: 63 HSSAGNIGGVYDITQRCWSTEAMAMLGIPPMLMPERLIASSDVVGPLLPSQAMALGLAAG 122 S + +++I W E + LGIP ++P+ + S+ + G + A LG A Sbjct: 180 DVSNASRTMLWNIHTGAWDEELLQWLGIPAAMLPQ-VQPSAHLYGEI---DAQHLGAAVK 235 Query: 123 TPVIAGGVDAAMATFAAGVTKAGNHVAMIGTSMCWGYI---NQQVDAGHGLISMPHVYNG 179 IAG D A F + G GT C+ + +Q + +GLIS Sbjct: 236 IGGIAG--DQQSALFGQACVRPGMAKNTYGTG-CFMLLHTGDQCATSHNGLISTAACQVD 292 Query: 180 LQDLYIFGGAI-TAGAAVSWFLEQFCQAEMTEALRSDVDPHFLLERDAAAIAPGAEGIVF 238 Q Y G++ GA V W + +A+++ D L A + G+VF Sbjct: 293 EQKQYALEGSVFIGGAVVQWLRDGL------KAIKTSTDVEGL-----ACSVEDSGGVVF 341 Query: 239 LPYLMGERGPIWDAKASGAFVGLSLYHRRAHLYRAVLEGVAFALKHNIDAGACGAQFLDE 298 +P G P W+ A GA VGLS AH+ RA LE +AF ++A A Sbjct: 342 VPSFTGLGAPYWNPSAKGAIVGLSRGSTVAHIARAALESIAFQSTALLNAMVRDAVSPIT 401 Query: 299 RLVVVGGASHSNLWMQIIADVTGYPVYTIDDNVEAALGAALLAAFGVGLI-DTSQVEKGW 357 L V GGA+ +NL +Q AD+ G PV ALGAA LA G+ D S++ W Sbjct: 402 ELRVDGGAAANNLLLQFQADLLGIPVIRPQVTETTALGAAYLAGIATGVYRDLSELSSQW 461 Query: 358 ARLRLRTTPDIAASRRYQHIFA 379 R+ P I + I A Sbjct: 462 -RMEHTFEPSIGRDQAQARIHA 482 >gi|300310399|ref|YP_003774491.1| D-ribulose/ribitol kinase [Herbaspirillum seropedicae SmR1] Length = 548 Score = 82.8 bits (203), Expect = 5e-17, Method: Compositional matrix adjust. Identities = 110/423 (26%), Positives = 173/423 (40%), Gaps = 44/423 (10%) Query: 15 GNGVDSYYG--------YTKMLWLKNRRPEVWAKTRYFLPPNSYVNYLLTGELAVDHSSA 66 G+ V SY G K+LWLK PEV+A +F ++ + TG L S Sbjct: 123 GHAVLSYVGGVISPEMETPKLLWLKENLPEVYASAAHFFDLTDFLTWKATGSLQ-RSSCT 181 Query: 67 GNIGGVYDITQRCWSTEAMAMLGIPPM-------LMPERLIASSDVVGPLLPSQAMALGL 119 Y + W +G+ + + E + + + G L + A A+GL Sbjct: 182 VTCKWTYLAHEAGWDASYFRQIGLGDLAEAGFARIGAEVVWPGTPLAGGLTQTAAQAMGL 241 Query: 120 AAGTPVIAGGVDAAMATFAAGVTKAGNHVAMIGTSMCWGYINQQVDAGHGLISMPHVYNG 179 G V AG +DA + G A + +G + + + + +P V+ Sbjct: 242 RPGIAVAAGLIDAHAGGVGTVAARGGQGDAAACMAYVFGTSSCTMTSHADPVLVPGVWGP 301 Query: 180 LQDLYI------FGGAITAGAAVSWFLEQFCQAEMT--EALRSDVD-PHFLLERDAAAIA 230 GG AGAA+ L EA R+ ++ P +L ++ AA+ Sbjct: 302 YYKAMAPGMWLNEGGQSAAGAAIDHLLRLHPATPQAREEAARAQMELPQWLAQQALAAVD 361 Query: 231 PGAEGI------VFLPYLMGERGPIWDAKASGAFVGLSLYH---RRAHLYRAVLEGVAFA 281 +E + +P +G R P+ D +A A VGL + H LY A L + + Sbjct: 362 HPSEAVWLAGQLTVVPEFLGNRSPLADPQARAALVGLGMEHDIDSLVALYVAGLCSLGYG 421 Query: 282 LKHNIDA-GACGAQFLDERLVVVGGASHSNLWMQIIADVTGYPVYTIDDNVEAALGAALL 340 L+ I+A ACG + + + V GGA L Q++AD TG PV LG+A+L Sbjct: 422 LRQIIEAQAACGVRV--QSISVSGGAGVHPLTRQLLADATGMPVEITASPEPVLLGSAML 479 Query: 341 AAFGVGL-IDTSQVEKGWARLRLRTTP---DIAA--SRRYQHIFAQYRGLYPSLKSAMHQ 394 AA GL D + + R+ P D+AA + RYQ F + + L L+ ++ Sbjct: 480 AAVAAGLHADLQSAMPAMSSVAGRSLPAQGDVAALHAARYQ-AFLKSQQLARELRDSLAP 538 Query: 395 LHA 397 L A Sbjct: 539 LLA 541 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.137 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,293,043 Number of extensions: 55023 Number of successful extensions: 186 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 172 Number of HSP's successfully gapped: 5 Length of query: 398 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 311 Effective length of database: 1,207,925 Effective search space: 375664675 Effective search space used: 375664675 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)