BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0225.0012.p (243 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310400|ref|YP_003774492.1| 2-deoxy-D-gluconate 3-dehydroge... 181 4e-47 gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reduc... 110 1e-25 gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 108 5e-25 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 102 2e-23 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 101 5e-23 >gi|300310400|ref|YP_003774492.1| 2-deoxy-D-gluconate 3-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 241 Score = 181 bits (459), Expect = 4e-47, Method: Compositional matrix adjust. Identities = 99/238 (41%), Positives = 145/238 (60%) Query: 3 FDVAKRKYVVTGATSGIGEVTARLLRSCGAAVVAVGRQKDKLALLKEEIGCETLVLDIAD 62 A + ++TGA GIG A LL GA V+A+ R LA L+EEIG + +D++D Sbjct: 1 MSFAGKTVIITGAGRGIGRACALLLAQRGAQVIALARTASDLARLQEEIGARVIAVDLSD 60 Query: 63 DRAMEVALQDVRGIDGLVNCSGISVLENSLALTSENFDRVMQVNARGTAMMSRHVAATMI 122 A A+ + D L+N +GI+VLE+ L ++ E ++ V+ VN R + + A I Sbjct: 61 PAAARRAMAEAGTADFLINSAGINVLESVLEMSDEGYEAVLGVNLRAALITCQAFARMRI 120 Query: 123 REGRSGSIVNVSSQAALVALDDHLAYCASKAAMDAITRVMCAELGPHRIRVNSVNPTVTL 182 +G G++VN++S A + HL Y ASKA ++ TRV+ ELGPH IRVN+V PT+TL Sbjct: 121 AQGGGGAMVNITSIAGHRGFEQHLCYAASKAGLEGATRVLARELGPHGIRVNAVAPTITL 180 Query: 183 TPMALQAWADPEKNRRVLEAIPLRKFAAPEDVAAPILFLLSDAAAMISGVSLPIDGGY 240 T +A AW+DP K+ ++ P +FA+PEDVA I LLS AAM++G +P+DGG+ Sbjct: 181 TELAEAAWSDPAKSGPMMARHPSARFASPEDVARSIAMLLSPDAAMLNGAVVPVDGGF 238 >gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 241 Score = 110 bits (274), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 84/241 (34%), Positives = 125/241 (51%), Gaps = 12/241 (4%) Query: 4 DVAKRKY-VVTGATSGIGEVTARLLRSCGAAVVAVGRQKDKLALLKEEI-GCE-TLV-LD 59 D+ K K +VTG T GIG AR L + GA V+A G EE G E TLV ++ Sbjct: 6 DLLKGKTALVTGGTQGIGAAVARQLVALGARVIAAGLPP----FAHEEFAGSEVTLVEMN 61 Query: 60 IADDRAMEVALQDVRGIDGLVNCSGISVLENSLALTSENFDRVMQVNARGTAMMSRHVAA 119 +A + ++ AL + +D +VNC+GI ++ L +E F++V+ VN GT M A Sbjct: 62 VASEESVRAALAGLTQLDIVVNCAGI--IKRGAELETEVFEQVIAVNLTGT-MRVCAAAR 118 Query: 120 TMIREGRSGSIVNVSSQAALVALDDHLAYCASKAAMDAITRVMCAELGPHRIRVNSVNPT 179 ++R+ R G IVN +S + Y ASK + +T+ + IRVN+V P Sbjct: 119 ELLRQSR-GCIVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVNAVAPG 177 Query: 180 VTLTPMALQAWADPEKNRRVLEAIPLRKFAAPEDVAAPILFLLSDAAAMISGVSLPIDGG 239 TP+ DP + +L PL ++ PEDV + FL S AA+ ++G +P+DGG Sbjct: 178 WIATPLTQALQDDPVRAGPILARTPLGRWGQPEDVGQVVAFLCSPAASFMTGAIVPVDGG 237 Query: 240 Y 240 Y Sbjct: 238 Y 238 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 108 bits (269), Expect = 5e-25, Method: Compositional matrix adjust. Identities = 82/241 (34%), Positives = 126/241 (52%), Gaps = 16/241 (6%) Query: 11 VVTGAT--SGIGEVTARLLRSCGAAVVAVGRQKDKLALLKEEIGCE--TLVLDIADDRAM 66 V+TG +G+G TARL+ GA VV + + A IG + LV D+ D + Sbjct: 9 VITGGAGINGLGYATARLMAEQGARVVVLDLAQADPAAAAARIGSQHLGLVADVTDKASC 68 Query: 67 EVA----LQDVRGIDGLVNCSGISVLENSLALTSENFDRVMQVNARGTAMMSRHVAATMI 122 E A LQ ID LVN +GI+ +L +T ++DR++ V+ RGT MS+ V M Sbjct: 69 ETAAAAILQQFGRIDILVNNAGITQPVKTLEITGADYDRILDVSLRGTLYMSQAVLPAM- 127 Query: 123 REGRSGSIVNVSSQAAL----VALDDHLAYCASKAAMDAITRVMCAELGPHRIRVNSVNP 178 R ++GSI+ +SS +A + H Y A+KA + + R M E GP IRVNS+ P Sbjct: 128 RAQQNGSIICISSVSAQRGGGILGGPH--YSAAKAGVLGLARAMAREFGPEGIRVNSITP 185 Query: 179 TVTLTPMALQAWADPEKNRRVLEAIPLRKFAAPEDVAAPILFLLSDAAAMISGVSLPIDG 238 + T + ++ EK + E IPL + +D+ +FL SD + +G++L ++G Sbjct: 186 GLIGTDI-IKGKLTEEKKAEIAETIPLARLGRADDIGGACVFLGSDLSQYCTGITLDVNG 244 Query: 239 G 239 G Sbjct: 245 G 245 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 102 bits (255), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 78/236 (33%), Positives = 118/236 (50%), Gaps = 9/236 (3%) Query: 11 VVTGATSGIGEVTARLLRSCGAAVVAVGRQKDKLALLKEEIGCETLVLDIAD-------D 63 +VTGA SGIGE TAR GA V+ R L + + + E D Sbjct: 18 IVTGAASGIGEATARRFSDEGARVLLADRDAAALGKVFDSLPPERTAARETDVSHHEQVR 77 Query: 64 RAMEVALQDVRGIDGLVNCSGISVLENSLALTSENFDRVMQVNARGTAMMSRHVAATMIR 123 + ++ A++ +D LV+ +G+ N ++ E++ RV N G +R + Sbjct: 78 QLVDFAIERFGQLDVLVSDAGVFAEGNVTEVSPEDWHRVQATNVNGVFYGAREALPHL-- 135 Query: 124 EGRSGSIVNVSSQAALVALDDHLAYCASKAAMDAITRVMCAELGPHRIRVNSVNPTVTLT 183 E G IVNV+S + L A + AY ASK A+ +TR M + G +R+N+V P++T T Sbjct: 136 EKTRGCIVNVASVSGLAADWNLSAYNASKGAVCNLTRAMALDFGRKGVRINAVCPSLTHT 195 Query: 184 PMALQAWADPEKNRRVLEAIPLRKFAAPEDVAAPILFLLSDAAAMISGVSLPIDGG 239 M DP + E I L + A P ++AA I FL S A+ ++GV+LP+DGG Sbjct: 196 AMTADMADDPPLLDKFAERIALGRGADPLEIAAVITFLASPDASFVNGVNLPVDGG 251 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 101 bits (252), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 80/241 (33%), Positives = 128/241 (53%), Gaps = 16/241 (6%) Query: 11 VVTGAT--SGIGEVTARLLRSCGAAVVAVGRQKDKLALLKEEIGCE--TLVLDIADDRAM 66 ++TG +G+G TARL+ + GA +V + ++ + A +G + LV D+ D A Sbjct: 9 IITGGAGQNGLGFSTARLMAAQGARIVILDLERAEPAGAAARLGAQHLGLVADVTDKAAC 68 Query: 67 EVA----LQDVRGIDGLVNCSGISVLENSLALTSENFDRVMQVNARGTAMMSRHVAATMI 122 E A L+ ID LVN +G++ L +T +++DR++ V+ RGT MS+ V M Sbjct: 69 EAAAAAVLKQFGRIDILVNNAGVTQPAKLLEITGKDYDRILDVSLRGTLYMSQAVLPAMQ 128 Query: 123 REGRSGSIVNVSSQAAL----VALDDHLAYCASKAAMDAITRVMCAELGPHRIRVNSVNP 178 + +SGSIV +SS +A + H Y A+KA + + R M E GP IRVN + P Sbjct: 129 AQ-QSGSIVCISSVSAQRGGGILGGPH--YSAAKAGVLGLARAMAREFGPQGIRVNCITP 185 Query: 179 TVTLTPMALQAWADPEKNRRVLEAIPLRKFAAPEDVAAPILFLLSDAAAMISGVSLPIDG 238 + T + + EK + + IPL + P+DV +FL SD + +GV+L ++G Sbjct: 186 GLISTDINKDILS-AEKKAEIAQTIPLGRLGGPDDVGGVCVFLASDLSQYCTGVTLDVNG 244 Query: 239 G 239 G Sbjct: 245 G 245 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.132 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 684,034 Number of extensions: 25497 Number of successful extensions: 229 Number of sequences better than 1.0e-08: 46 Number of HSP's gapped: 161 Number of HSP's successfully gapped: 46 Length of query: 243 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 160 Effective length of database: 1,226,865 Effective search space: 196298400 Effective search space used: 196298400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)