BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0225.0007.p
         (221 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300314064|ref|YP_003778156.1| threonine efflux protein [Herba...   280   5e-77
gi|300312440|ref|YP_003776532.1| threonine efflux protein [Herba...    75   5e-15
gi|300311201|ref|YP_003775293.1| threonine efflux protein [Herba...    55   5e-09

>gi|300314064|ref|YP_003778156.1| threonine efflux protein
           [Herbaspirillum seropedicae SmR1]
          Length = 210

 Score =  280 bits (717), Expect = 5e-77,   Method: Compositional matrix adjust.
 Identities = 164/221 (74%), Positives = 187/221 (84%), Gaps = 11/221 (4%)

Query: 1   MQALFHTIGITDVWQLIAATVVFLMLPGPGTFCVLTCSAKHGLRGGFXGTCSAKHGLRGG 60
           MQALFH++GITD+WQLIAAT+VFL+LPGPGTFCVLTC+AK G+RGGF             
Sbjct: 1   MQALFHSLGITDIWQLIAATMVFLLLPGPGTFCVLTCAAKGGVRGGFAAQ---------- 50

Query: 61  FAGLMLGDIVLMFLAAIGVAALLHANPLLFKGMQYVGAAYLAYLGGRLLFAKEEGGSSVV 120
            AGLMLGD+VLMFLAA GVAALLHANPLLF G+QY+GAAYLAYLG RLLFA+ EGG +VV
Sbjct: 51  -AGLMLGDVVLMFLAAAGVAALLHANPLLFHGLQYLGAAYLAYLGARLLFARGEGGGTVV 109

Query: 121 PFSDMEDFRRGFFVTLMNPKAIVFYMAFFPLFIDPATQKGAVTFLIMGTVICTCTLIYGS 180
           PFS+  +FRRGF VTL NPKAIVFYMAFFPLF+DPATQ+GA+TF+ MG VI  CT +YGS
Sbjct: 110 PFSNAAEFRRGFLVTLFNPKAIVFYMAFFPLFLDPATQQGALTFITMGAVISCCTFLYGS 169

Query: 181 LLVLAGNAAAKRLARNRKIAALASRAAGVFLIGFGIKLGTH 221
           +LV+ GNAAA+R+ARNR IA LASRAAGVFLIGFGIKL T 
Sbjct: 170 VLVMVGNAAARRMARNRTIARLASRAAGVFLIGFGIKLTTQ 210


>gi|300312440|ref|YP_003776532.1| threonine efflux protein
           [Herbaspirillum seropedicae SmR1]
          Length = 208

 Score = 74.7 bits (182), Expect = 5e-15,   Method: Compositional matrix adjust.
 Identities = 60/218 (27%), Positives = 91/218 (41%), Gaps = 21/218 (9%)

Query: 10  ITDVWQLIAATVVFLMLPGPGTFCVLTCSAKHGLRGGFXGTCSAKHGLRGGFAGLMLGDI 69
           + D+   + A +   + PGP    VLT     G + G               AG   G +
Sbjct: 1   MPDLILFLLAAMTLTLAPGPDNLYVLTRGIAQGRKAGLVAA-----------AGFCSGLV 49

Query: 70  VLMFLAAIGVAALLHANPLLFKGMQYVGAAYLAYLGGRLL--------FAKEEGGSSVVP 121
               LA +G AAL+ A P  +  +QY GAAYLAYLG R L         A +  G++ VP
Sbjct: 50  FHTLLAVLGFAALIKAYPPAYHALQYAGAAYLAYLGVRTLRSASAGLALAADGQGAAAVP 109

Query: 122 FSDMEDFRRGFFVTLMNPKAIVFYMAFFPLFIDPATQKGAVTFLIMGTVICTCTLIYGSL 181
              +  + +     ++NPK  +F++AF P F+       A+  L++  V         S 
Sbjct: 110 LRRI--YWQSVLANMLNPKVTLFFIAFLPQFVQREAGHEALQMLVLALVFIVQAFAIFSA 167

Query: 182 LVLAGNAAAKRLARNRKIAALASRAAGVFLIGFGIKLG 219
           + L   A      R    +   +R AG   IG GI++ 
Sbjct: 168 IALFSGAVGAYFRRRASASVHLNRLAGCAFIGLGIRMA 205


>gi|300311201|ref|YP_003775293.1| threonine efflux protein
           [Herbaspirillum seropedicae SmR1]
          Length = 219

 Score = 55.1 bits (131), Expect = 5e-09,   Method: Compositional matrix adjust.
 Identities = 56/182 (30%), Positives = 81/182 (44%), Gaps = 29/182 (15%)

Query: 28  GPGTFCVLTCSAKHGLRGGFXGTCSAKHGLRGGFAGLMLGDIVLMFLAAIGVAALLHANP 87
            PG+  VL+ S  HGL  G   T +          GL +  +V++ +A  GV +LL A+ 
Sbjct: 19  SPGSGAVLSMS--HGLNYGVRRTSAT-------ILGLEIALVVILIIAGAGVGSLLVASE 69

Query: 88  LLFKGMQYVGAAYLAYLG---GRLLFAKEEGGSSV-------VPFSDMEDFRR---GFFV 134
             F  ++  GA YL YLG    R     EE G ++          +    ++R   GF  
Sbjct: 70  TAFNVIKVAGALYLIYLGFSQWRARVPSEEQGEALDAANASARAHARQTWWQRCMTGFLT 129

Query: 135 TLMNPKAIVFYMAFFPLFIDPATQKGAVTFLIMGTVICTCTLIYGSLLVLAGNA-AAKRL 193
              NPK IVF +A  P FID          L +  ++   T++   L+V+ G A AA RL
Sbjct: 130 NATNPKGIVFMVAVLPQFIDHDRP------LWLQLLVLAVTMVAVDLVVMHGYAFAASRL 183

Query: 194 AR 195
            R
Sbjct: 184 QR 185


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.331    0.145    0.446 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 590,522
Number of extensions: 21473
Number of successful extensions: 84
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 83
Number of HSP's successfully gapped: 3
Length of query: 221
Length of database: 1,619,870
Length adjustment: 82
Effective length of query: 139
Effective length of database: 1,231,600
Effective search space: 171192400
Effective search space used: 171192400
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 129 (54.3 bits)