BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0223.0025.p (138 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313033|ref|YP_003777125.1| hypothetical protein Hsero_3739... 196 7e-52 >gi|300313033|ref|YP_003777125.1| hypothetical protein Hsero_3739 [Herbaspirillum seropedicae SmR1] Length = 130 Score = 196 bits (497), Expect = 7e-52, Method: Compositional matrix adjust. Identities = 91/113 (80%), Positives = 104/113 (92%) Query: 25 FRFDERRLSLKGESYPENAAQFWGDIIVALRRFLAEDGDTAPVAVNVALAYFNSSSTKML 84 FRF++R+LSLKGESYPENAAQFWG+II LRRFLA+D AP+ VNVALAYFNSSSTKML Sbjct: 17 FRFEQRQLSLKGESYPENAAQFWGEIIATLRRFLADDSADAPLTVNVALAYFNSSSTKML 76 Query: 85 FSLFGALNDKAKSGTEVVLNWYHDEDDDTIFEFGQELREDFPDLVFNDHAVKS 137 FSLF ALN+KA++GTEV+LNWYHDEDDDTIFEFGQEL+EDFP LVF+DHAV + Sbjct: 77 FSLFDALNEKAEAGTEVILNWYHDEDDDTIFEFGQELQEDFPALVFHDHAVSN 129 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 344,115 Number of extensions: 11489 Number of successful extensions: 35 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 35 Number of HSP's successfully gapped: 1 Length of query: 138 Length of database: 1,619,870 Length adjustment: 76 Effective length of query: 62 Effective length of database: 1,260,010 Effective search space: 78120620 Effective search space used: 78120620 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)