BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0223.0017.p (265 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313109|ref|YP_003777201.1| short-chain alcohol dehydrogena... 348 2e-97 gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herb... 132 3e-32 gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogena... 129 2e-31 gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independe... 128 5e-31 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 123 2e-29 >gi|300313109|ref|YP_003777201.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 266 Score = 348 bits (893), Expect = 2e-97, Method: Compositional matrix adjust. Identities = 156/264 (59%), Positives = 206/264 (78%) Query: 1 MSERMKGKTVIVVGAGSSGPGWGNGKAAAALYAREGAKVFAVDFRLEAAQETESIIRAEG 60 M + M+GK +IV GAGS G GWGNGKAAA LYAREG +V AVD +EAA +T+ IIR+EG Sbjct: 1 MDKLMQGKAIIVTGAGSVGEGWGNGKAAAVLYAREGGRVLAVDRSIEAALQTQDIIRSEG 60 Query: 61 GECSSFRADVVSSEDLRDMAAACVEQYGQIDVLHNNVGIVETGGPVETSEESWNRVIAVN 120 G C ADV + +++ +A A ++ +G++DVLHNNVGI + GGPVETSEESWNR++AVN Sbjct: 61 GVCEVLAADVSRNTEVKAIAEAAMDHFGRVDVLHNNVGIADVGGPVETSEESWNRLVAVN 120 Query: 121 QTSVFLACKHVLPYMTARNSGAIVNIGSIAGVRWIGFPYVAYSAAKAALISMTTNIAVQY 180 QTS+FL KHVLP M + GAIVNI S+A +RWIGFPY+ Y+A KAA+++ T N+A+QY Sbjct: 121 QTSLFLTHKHVLPIMEKQRKGAIVNISSLAALRWIGFPYLGYTATKAAILAFTKNVAMQY 180 Query: 181 AAQGVRANCVLPGLMNTPLIREPLAKTYGGNVDSMIEQRDKLSPTGKMGDAWDTAYAALY 240 A G+RANCVLPG+M+TP+IREPL +YGG+++ M +R+ P G MGDAWD A+A+L+ Sbjct: 181 APMGIRANCVLPGMMDTPMIREPLKASYGGDIEQMRAKRNAQCPMGFMGDAWDVAHASLF 240 Query: 241 LASEEARYVNGTSIVVDGGLTARC 264 LAS+ ARY+ G ++VDGGL+ RC Sbjct: 241 LASDHARYITGLDMIVDGGLSLRC 264 >gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 132 bits (332), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 90/262 (34%), Positives = 136/262 (51%), Gaps = 22/262 (8%) Query: 5 MKGKTVIVVGAGSSGPGWGNGKAAAALYAREGAKVFAVDFRLEAAQETESIIRAEGGECS 64 ++GK ++ GA + G G+A A L+AR GA + R +A ++ ++I A+GG S Sbjct: 4 LQGKVALITGASA-----GIGRATALLFARHGAALILTARRQDALEQVAALICAQGGRAS 58 Query: 65 SFRADVVSSEDLRDMAAACVEQYGQIDVLHNNVGIVETGGPV-ETSEESWNRVIAVNQTS 123 DV + A ++++G +D+ NN G V P+ E S + W + N TS Sbjct: 59 VVAGDVGEAATHERGVAMALDEFGGLDIAINNAGAVGACKPLAEISPQQWQDTLHANLTS 118 Query: 124 VFLACKHVLPYMTARNSGAIVNIGSIAGVRWIGFP-YVAYSAAKAALISMTTNIAVQYAA 182 FL + +P M AR +GAIV S G G P AY AAKA L+ + IA YA Sbjct: 119 AFLGARCQIPAMLARGAGAIVFTSSFVGSS-AGLPGMAAYGAAKAGLMGLVKGIAADYAV 177 Query: 183 QGVRANCVLPGLMNTPLIREPLAKTYGGNVDSM--IEQRDKLSPTGKMGDAWDTAYAALY 240 QG+RAN +LPG ++T + +P K + + +M I Q +++ A AAL+ Sbjct: 178 QGIRANALLPGGVDTDMGGDPQQKQWAAGLHAMKRIAQPEEI------------AAAALF 225 Query: 241 LASEEARYVNGTSIVVDGGLTA 262 LAS A +V GT++ DGG +A Sbjct: 226 LASPMASFVTGTALYADGGNSA 247 >gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 129 bits (325), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 86/260 (33%), Positives = 138/260 (53%), Gaps = 19/260 (7%) Query: 4 RMKGKTVIVVGAGSSGPGWGNGKAAAALYAREGAKVFAVDFRLEAAQETESIIRAEGGEC 63 R+ GKTV++ A G G+A+ L+AREGA+V A D E I E Sbjct: 7 RLAGKTVLITAAAQ-----GIGRASTELFAREGARVIATDISKPHLDELAGIAGVE---- 57 Query: 64 SSFRADVVSSEDLRDMAAACVEQYGQIDVLHNNVGIVETGGPVETSEESWNRVIAVNQTS 123 + DV ++ A V + G IDVL N G V G +E +++W+ +N + Sbjct: 58 -THLLDVTDDAAIK----ALVAKIGTIDVLFNCAGYVAAGNILECDDKAWDFSFNLNAKA 112 Query: 124 VFLACKHVLPYMTARNSGAIVNIGSIA-GVRWIGFPYVAYSAAKAALISMTTNIAVQYAA 182 +F + VLP M A+ +G+IVNI S A V+ + + AY A+KAA++ +T ++A + A Sbjct: 113 MFHTIRAVLPGMLAKKAGSIVNIASAASSVKGVANRF-AYGASKAAVVGLTKSVAADFVA 171 Query: 183 QGVRANCVLPGLMNTPLIREPL---AKTYGGNVDSMIEQRDKLSPTGKMGDAWDTAYAAL 239 QG+R N + PG + +P + + + AK G + + + P G++G A + A AL Sbjct: 172 QGIRCNAICPGTIESPSLNQRISTQAKETGKSEEEVRAAFVGRQPMGRIGKAEEVAALAL 231 Query: 240 YLASEEARYVNGTSIVVDGG 259 YLAS+E+ + G+ ++DGG Sbjct: 232 YLASDESNFTTGSIHMIDGG 251 >gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independent alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 128 bits (322), Expect = 5e-31, Method: Compositional matrix adjust. Identities = 84/242 (34%), Positives = 120/242 (49%), Gaps = 16/242 (6%) Query: 29 AALYAREGAKVFAVDFRLEAAQETESIIRAEGGECSSFRADVVSSEDLRDMAAACVEQYG 88 A YAREGA + D I+A GG +ADV D+ + A +E +G Sbjct: 22 AKAYAREGAAIVVADIGEAGGLRVVEEIKAAGGRAVFAKADVSKRADMDQLLATALEHFG 81 Query: 89 QIDVLHNNVGIVETGGP-VETSEESWNRVIAVNQTSVFLACKHVLPYMTARNSGAIVNIG 147 ++D++ NN G P +E E+ ++RV AVN S+FL+ K +PY GA +NI Sbjct: 82 KLDIVVNNAGTTHRNRPMLEVEEDEFDRVYAVNVKSIFLSAKTFVPYFRQVGGGAFINIA 141 Query: 148 SIAGVR-WIGFPYVAYSAAKAALISMTTNIAVQYAAQGVRANCVLPGLMNTPLIREPLAK 206 S AG+R G + Y+ +K A+I+ + ++A + +R NCV P + T L+ E + Sbjct: 142 STAGIRPRPGLTW--YNGSKGAVITTSKSMAAELGPDKIRVNCVNPVISATGLLSEFMG- 198 Query: 207 TYGGNVDSMIEQRDKLS---PTGKMGDAWDTAYAALYLASEEARYVNGTSIVVDGGLTAR 263 V E R K P G+ D A A LYL S+EA +V G I VDGG R Sbjct: 199 -----VPDTPENRKKFVATIPMGRFSTPEDIANACLYLGSDEAEFVTGVCIEVDGG---R 250 Query: 264 CV 265 CV Sbjct: 251 CV 252 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 123 bits (308), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 83/265 (31%), Positives = 133/265 (50%), Gaps = 25/265 (9%) Query: 3 ERMKGKTVIVVGAGSSGPGWGNGKAAAALYAREGAKVFAVDFRLEAAQETESIIRAEGGE 62 +R +GK VIV GA S G G+A A ++ EGA+V D A + + E Sbjct: 10 QRFEGKVVIVTGAAS-----GIGEATARRFSDEGARVLLADRDAAALGKVFDSLPPE--R 62 Query: 63 CSSFRADVVSSEDLRDMAAACVEQYGQIDVLHNNVGIVETGGPVETSEESWNRVIAVNQT 122 ++ DV E +R + +E++GQ+DVL ++ G+ G E S E W+RV A N Sbjct: 63 TAARETDVSHHEQVRQLVDFAIERFGQLDVLVSDAGVFAEGNVTEVSPEDWHRVQATNVN 122 Query: 123 SVFLACKHVLPYMTARNSGAIVNIGSIAGVRWIGFPYVAYSAAKAALISMTTNIAVQYAA 182 VF + LP++ + G IVN+ S++G+ + AY+A+K A+ ++T +A+ + Sbjct: 123 GVFYGAREALPHLE-KTRGCIVNVASVSGL-AADWNLSAYNASKGAVCNLTRAMALDFGR 180 Query: 183 QGVRANCVLPGLMNTPLIRE-----PLAKTYGGNVDSMIEQRDKLSPTGKMGDAWDTAYA 237 +GVR N V P L +T + + PL + + G+ D + A Sbjct: 181 KGVRINAVCPSLTHTAMTADMADDPPLLDKFAERI-----------ALGRGADPLEIAAV 229 Query: 238 ALYLASEEARYVNGTSIVVDGGLTA 262 +LAS +A +VNG ++ VDGGL A Sbjct: 230 ITFLASPDASFVNGVNLPVDGGLMA 254 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.131 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 718,112 Number of extensions: 26950 Number of successful extensions: 204 Number of sequences better than 1.0e-08: 47 Number of HSP's gapped: 127 Number of HSP's successfully gapped: 47 Length of query: 265 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 181 Effective length of database: 1,222,130 Effective search space: 221205530 Effective search space used: 221205530 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)