BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0223.0013.p
         (416 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300309915|ref|YP_003774007.1| alkaline phosphatase D [Herbasp...   477   e-135

>gi|300309915|ref|YP_003774007.1| alkaline phosphatase D
           [Herbaspirillum seropedicae SmR1]
          Length = 542

 Score =  477 bits (1227), Expect = e-135,   Method: Compositional matrix adjust.
 Identities = 225/289 (77%), Positives = 252/289 (87%), Gaps = 3/289 (1%)

Query: 78  APAVVTSERMRPQVDSGAMSGDITRDTAMLWSRTDRAARMVVEYSVNENFKDAFTVVGPA 137
           APA+VTSER+RPQ+  G MSGD+TRD+A+LW+R DR ARM+VEYS + NF +   + GP 
Sbjct: 42  APAIVTSERLRPQLPGGVMSGDVTRDSAILWARADRNARMLVEYSTSGNFSNPVRIAGPT 101

Query: 138 ALARNDYTARMDLRGLPVSQRLYYRLRFQDLQHESAWSAPVTGSLIIPG---GGERDISF 194
           ALAR DYTAR+DLR LP  QRLYYR+RFQDL HESA S P+TGSL++P    G  RDI+F
Sbjct: 102 ALAREDYTARVDLRDLPAGQRLYYRVRFQDLVHESALSEPLTGSLMMPASAHGQARDITF 161

Query: 195 AFSGDEAGQGWGINEAWGGYRVYETMRRFKPDFFIHSGDQIYADGPIQAEVTLDDGSLWR 254
           AFSGDEAGQGWGINE WGGYRVYE+MRRF+PDFFIHSGDQIYADGPIQAEV LDDGSLWR
Sbjct: 162 AFSGDEAGQGWGINEQWGGYRVYESMRRFRPDFFIHSGDQIYADGPIQAEVKLDDGSLWR 221

Query: 255 NLVTPAKSKVAQTLDDYRGNFAYNLMDANKKRFMAEVPFLVQWDDHEVRNNWYPGQVIGA 314
           N+VT AKSKVAQTLDDYRGNFAYN++DANK+RF AEVPFLVQWDDHEVRNNWYPGQ IGA
Sbjct: 222 NVVTEAKSKVAQTLDDYRGNFAYNMLDANKRRFAAEVPFLVQWDDHEVRNNWYPGQEIGA 281

Query: 315 AEKRYQERELNVLKQRARQAMFEYNPFRIDVADPERIYRSFNYGPLLEV 363
           AEKRY ER ++VL  RARQAMFE+NPFRID ADP RIYRSFN+GPLLEV
Sbjct: 282 AEKRYDERNIDVLAARARQAMFEHNPFRIDSADPFRIYRSFNHGPLLEV 330


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.319    0.134    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,288,142
Number of extensions: 54962
Number of successful extensions: 139
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 138
Number of HSP's successfully gapped: 1
Length of query: 416
Length of database: 1,619,870
Length adjustment: 88
Effective length of query: 328
Effective length of database: 1,203,190
Effective search space: 394646320
Effective search space used: 394646320
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 132 (55.5 bits)