BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0223.0010.p (161 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reduc... 291 2e-80 gi|300310400|ref|YP_003774492.1| 2-deoxy-D-gluconate 3-dehydroge... 105 2e-24 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 100 5e-23 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 100 8e-23 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 97 7e-22 >gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 241 Score = 291 bits (744), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 137/161 (85%), Positives = 150/161 (93%) Query: 1 VINCAGIIKRGAEHEVEVFEQVIAVNLTGTMRICSAARALLKESKGCIVNLASMLSFFGG 60 V+NCAGIIKRGAE E EVFEQVIAVNLTGTMR+C+AAR LL++S+GCIVN ASMLSFFGG Sbjct: 81 VVNCAGIIKRGAELETEVFEQVIAVNLTGTMRVCAAARELLRQSRGCIVNTASMLSFFGG 140 Query: 61 GLVPAYSSSKGGVAQLTKSLAIAYAADGIRVNAVAPGWIATPLTQALQEDPARSGPILQR 120 GLVP YS+SKGGVAQLTKSLAIAYAADGIRVNAVAPGWIATPLTQALQ+DP R+GPIL R Sbjct: 141 GLVPGYSASKGGVAQLTKSLAIAYAADGIRVNAVAPGWIATPLTQALQDDPVRAGPILAR 200 Query: 121 TPLNRWGTPEDIGNVAAFLCTPAASFMTGAVVPVDGGYLVS 161 TPL RWG PED+G V AFLC+PAASFMTGA+VPVDGGYLV+ Sbjct: 201 TPLGRWGQPEDVGQVVAFLCSPAASFMTGAIVPVDGGYLVA 241 >gi|300310400|ref|YP_003774492.1| 2-deoxy-D-gluconate 3-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 241 Score = 105 bits (261), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 66/163 (40%), Positives = 92/163 (56%), Gaps = 4/163 (2%) Query: 1 VINCAGI--IKRGAEHEVEVFEQVIAVNLTGTMRICSA-ARALLKESKG-CIVNLASMLS 56 +IN AGI ++ E E +E V+ VNL + C A AR + + G +VN+ S+ Sbjct: 77 LINSAGINVLESVLEMSDEGYEAVLGVNLRAALITCQAFARMRIAQGGGGAMVNITSIAG 136 Query: 57 FFGGGLVPAYSSSKGGVAQLTKSLAIAYAADGIRVNAVAPGWIATPLTQALQEDPARSGP 116 G Y++SK G+ T+ LA GIRVNAVAP T L +A DPA+SGP Sbjct: 137 HRGFEQHLCYAASKAGLEGATRVLARELGPHGIRVNAVAPTITLTELAEAAWSDPAKSGP 196 Query: 117 ILQRTPLNRWGTPEDIGNVAAFLCTPAASFMTGAVVPVDGGYL 159 ++ R P R+ +PED+ A L +P A+ + GAVVPVDGG+L Sbjct: 197 MMARHPSARFASPEDVARSIAMLLSPDAAMLNGAVVPVDGGFL 239 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 100 bits (250), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 57/162 (35%), Positives = 97/162 (59%), Gaps = 4/162 (2%) Query: 1 VINCAGIIKRGA--EHEVEVFEQVIAVNLTGTMRI-CSAARALLKESKGCIVNLASMLSF 57 ++N AG+ + + ++ +E + Q + VN+TG + AAR ++K+ G IVN++S+ Sbjct: 89 LVNNAGVARTYSFLDYPLENWLQTMNVNVTGVLLAGQHAARLMVKQQWGRIVNISSISGI 148 Query: 58 FGGGLVPAYSSSKGGVAQLTKSLAIAYAADGIRVNAVAPGWIATPLTQALQEDPARSGPI 117 G AY +SK V LT+ +AI A GI VN+VAPG + TP+TQA+ + R Sbjct: 149 RAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITVNSVAPGPVDTPMTQAMHSEQTRE-SY 207 Query: 118 LQRTPLNRWGTPEDIGNVAAFLCTPAASFMTGAVVPVDGGYL 159 + P+ R+GT ++I + +L ++++TG V+PVDGG++ Sbjct: 208 YRLVPMRRYGTTDEIASAVCYLAAEESAYITGHVIPVDGGFV 249 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 100 bits (248), Expect = 8e-23, Method: Compositional matrix adjust. Identities = 57/163 (34%), Positives = 89/163 (54%), Gaps = 2/163 (1%) Query: 1 VINCAGIIKRGAEHEV--EVFEQVIAVNLTGTMRICSAARALLKESKGCIVNLASMLSFF 58 +++ AG+ G EV E + +V A N+ G A L++++GCIVN+AS+ Sbjct: 93 LVSDAGVFAEGNVTEVSPEDWHRVQATNVNGVFYGAREALPHLEKTRGCIVNVASVSGLA 152 Query: 59 GGGLVPAYSSSKGGVAQLTKSLAIAYAADGIRVNAVAPGWIATPLTQALQEDPARSGPIL 118 + AY++SKG V LT+++A+ + G+R+NAV P T +T + +DP Sbjct: 153 ADWNLSAYNASKGAVCNLTRAMALDFGRKGVRINAVCPSLTHTAMTADMADDPPLLDKFA 212 Query: 119 QRTPLNRWGTPEDIGNVAAFLCTPAASFMTGAVVPVDGGYLVS 161 +R L R P +I V FL +P ASF+ G +PVDGG + S Sbjct: 213 ERIALGRGADPLEIAAVITFLASPDASFVNGVNLPVDGGLMAS 255 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 97.1 bits (240), Expect = 7e-22, Method: Compositional matrix adjust. Identities = 60/160 (37%), Positives = 93/160 (58%), Gaps = 5/160 (3%) Query: 1 VINCAGIIKR--GAEHEVEVFEQVIAVNLTGTMRICSAA-RALLKESKGCIVNLASMLSF 57 ++N AGI + + E ++ VIA NLT R+ A R ++K G I+N+ S++ Sbjct: 90 LVNNAGITQDQLAMRMKDEEWDSVIATNLTAVGRLSRAVLRGMMKARTGRIINITSVVGE 149 Query: 58 FGGGLVPAYSSSKGGVAQLTKSLAIAYAADGIRVNAVAPGWIATPLTQALQEDPARSGPI 117 G Y+++K GVA ++++LA + I VN VAPG+I T +T+AL E +S I Sbjct: 150 AGNPGQMNYAAAKAGVAGMSRALAREIGSRNITVNCVAPGFIDTDMTRALNEQ--QSAAI 207 Query: 118 LQRTPLNRWGTPEDIGNVAAFLCTPAASFMTGAVVPVDGG 157 LQ+ PL R G E+I AFL +P A+++TG + V+GG Sbjct: 208 LQQIPLGRLGAAEEIAYAVAFLASPQAAYITGTTLNVNGG 247 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 523,782 Number of extensions: 21578 Number of successful extensions: 158 Number of sequences better than 1.0e-08: 37 Number of HSP's gapped: 132 Number of HSP's successfully gapped: 37 Length of query: 161 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 83 Effective length of database: 1,250,540 Effective search space: 103794820 Effective search space used: 103794820 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)