BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0223.0009.p (578 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309918|ref|YP_003774010.1| major facilitator superfamily p... 756 0.0 gi|300312451|ref|YP_003776543.1| D-galactonate transporter prote... 192 4e-50 gi|300313884|ref|YP_003777976.1| phthalate permease of the major... 159 6e-40 gi|300313744|ref|YP_003777836.1| D-glucarate permease [Herbaspir... 129 6e-31 gi|300313393|ref|YP_003777485.1| major facilitator superfamily p... 124 1e-29 >gi|300309918|ref|YP_003774010.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 756 bits (1953), Expect = 0.0, Method: Compositional matrix adjust. Identities = 373/405 (92%), Positives = 390/405 (96%), Gaps = 2/405 (0%) Query: 1 MEVQK--SPKLKRIQIVALTFLMLAGIVNYLDRSTLSIANHSVSQELGLSASEMGLLLSA 58 MEVQK SP+LKRIQ+VAL+FLMLAGIVNYLDRSTLSIANHSVSQEL LSAS+MGLLLSA Sbjct: 1 MEVQKEKSPRLKRIQVVALSFLMLAGIVNYLDRSTLSIANHSVSQELNLSASQMGLLLSA 60 Query: 59 FSLAYAFSQLPIGAMLDKFGARVMLGAGMMVWSVAQLAGGFVGTLHQFLVARVFLGLGEA 118 FSLAYAFSQLPIGAMLDKFGARVMLGAGM +WSVAQLAGGFV L QFLVARVFLGLGEA Sbjct: 61 FSLAYAFSQLPIGAMLDKFGARVMLGAGMFIWSVAQLAGGFVNNLQQFLVARVFLGLGEA 120 Query: 119 PQFPAGAKVVSEWFAVRERGGPTGIFVASSTIGPALAPPILTVLMLSFGWRMMFVIMGIA 178 PQFPAGAKVVSEWFA+RERGGPTGIFVASSTIGPALAPPILT+LMLSFGWRMMFVIMG+ Sbjct: 121 PQFPAGAKVVSEWFAMRERGGPTGIFVASSTIGPALAPPILTILMLSFGWRMMFVIMGVL 180 Query: 179 GIAVAVGWYIFYRNRNEVELTPSEILYLADGTDNDPNANAKLTWHEWKGLFKYRTTWGML 238 GIAV+VGWY+FYRNRNEV+LTPSE+LYLAD DNDPNANAKL+W EWK LF YRTTWGML Sbjct: 181 GIAVSVGWYLFYRNRNEVQLTPSEVLYLADDHDNDPNANAKLSWSEWKSLFTYRTTWGML 240 Query: 239 FGFMGVIYMVWLYLTWLPAYLEHERHLSIARTGWVVVIPYIFGTLGMLSSGYIADGLMKR 298 FGFMGVIYMVWLYLTWLPAYLEHERHLSIARTGWVVVIPYIFGTLGMLSSGYIADGLMKR Sbjct: 241 FGFMGVIYMVWLYLTWLPAYLEHERHLSIARTGWVVVIPYIFGTLGMLSSGYIADGLMKR 300 Query: 299 GMAPIRSRKWPICAGLIGAGAFTIPAAYTPSTTMAIVYISLAMAFVNMASGGAWALVSVA 358 GM IRSRKWPICAGLIGAGAFTIPAAYTPSTTMAIVYISLAMAFVNMASGGAWALVSVA Sbjct: 301 GMPAIRSRKWPICAGLIGAGAFTIPAAYTPSTTMAIVYISLAMAFVNMASGGAWALVSVA 360 Query: 359 IPRRLVASLGSLQNFGGYLGGSLAPLITGIVVDKTHSFVNALLIS 403 IPRRLVASLGS+QNFGGYLGGSLAP+ITG+VVD+THSFVNALLIS Sbjct: 361 IPRRLVASLGSMQNFGGYLGGSLAPVITGVVVDQTHSFVNALLIS 405 >gi|300312451|ref|YP_003776543.1| D-galactonate transporter protein [Herbaspirillum seropedicae SmR1] Length = 439 Score = 192 bits (489), Expect = 4e-50, Method: Compositional matrix adjust. Identities = 129/408 (31%), Positives = 208/408 (50%), Gaps = 17/408 (4%) Query: 8 KLKRIQIVALTFLMLAGIVNYLDRSTLSIANHSVSQELGLSASEMGLLLSAFSLAYAFSQ 67 K ++ + L L + VNY DR+TLSIA ++ EL L MG + SAFS AY +Q Sbjct: 7 KPTHVRYLILFLLFVVTTVNYADRATLSIAGSAMKTELSLDPVMMGYIFSAFSWAYVLAQ 66 Query: 68 LPIGAMLDKFGARVMLGAGMMVWSVAQLAGGFV------GTLHQFLVARVFLGLGEAPQF 121 LP G +LD+FG++ + + +WSV V + + R +GL EAP F Sbjct: 67 LPGGWLLDRFGSKKIYMWSIALWSVFTFLQAGVTWMSTAAAVASLFLLRFMVGLAEAPSF 126 Query: 122 PAGAKVVSEWFAVRERGGPTGIFVASSTIGPALAPPILTVLMLSFGWRMMFVIMGIAGIA 181 PA ++V+ WF +ERG + IF ++ L P++ ++ ++ W +F +MG+ GIA Sbjct: 127 PANGRIVAAWFPTKERGTASAIFNSAQYFAAVLFTPLMAWIVHTYSWHHVFTVMGVVGIA 186 Query: 182 VAVGW-YIFYRNRNEVELTPSEILYLADGTDNDPNANAKLTWHEWKG------LFKYRTT 234 +A+ W I Y ++ + +E+ ++ G N K+ KG L R Sbjct: 187 LALIWGRIIYSPKDHPRINQAELAHIESGGGLVDMDNRKVAAPSTKGRGYIRQLLSNRML 246 Query: 235 WGMLFGFMGVIYMVWLYLTWLPAYLEHERHLSIARTGWVVVIPYIFGTLGMLSSGYIADG 294 G+ G + + + +LTW P YL ER +SI + G+V IP + G +G + G I+D Sbjct: 247 LGVYIGQYCINVITYFFLTWFPVYLVQERGMSILKAGFVASIPAVCGFIGGVVGGLISDR 306 Query: 295 LMKRGMAPIRSRKWPICAGLIGAGAFTIPAAYTPSTTMAIVYISLAMAFVNMASGG-AWA 353 L+KRG++ +RK PI G++ + I Y + M + +A+AF G WA Sbjct: 307 LIKRGLSVTWARKIPIVGGMLLSMTM-ILCNYVEAQWMVVGI--MALAFFGKGVGALGWA 363 Query: 354 LVSVAIPRRLVASLGSLQNFGGYLGGSLAPLITGIVVDKTHSFVNALL 401 +V+ P+++V G L N G + G P++ G +V +T SF AL+ Sbjct: 364 VVADTAPKQIVGLSGGLFNMFGNVAGITTPIVIGYIVKETGSFSGALV 411 >gi|300313884|ref|YP_003777976.1| phthalate permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 418 Score = 159 bits (402), Expect = 6e-40, Method: Compositional matrix adjust. Identities = 112/388 (28%), Positives = 187/388 (48%), Gaps = 10/388 (2%) Query: 9 LKRIQIVALTFLMLAGIVNYLDRSTLSIANHSVSQELGLSASEMGLLLSAFSLAYAFSQL 68 ++ I+ +T + LA IVNYL R+TLS+A ++ +EL +S + ++ A+ + YA Q Sbjct: 4 VRGIRWYMVTLVTLALIVNYLARNTLSVAAPTMMKELDMSTQQYSYIVVAWQICYAVMQP 63 Query: 69 PIGAMLDKFGARVMLGAGMMVWSVAQLAGGFVGTLHQFLVARVFLGLGEAPQFPAGAKVV 128 G +LD G ++ G + WS+ A F R LG+ EA P G K Sbjct: 64 VAGYILDAVGTKIGFGIFALAWSLVCAAAAFATGWQSLAFFRALLGITEAAGIPGGVKAS 123 Query: 129 SEWFAVRERGGPTGIFVASSTIGPALAPPILTVLMLSFGWRMMFVIMGIAGIAVAVGWYI 188 +EWF +ER G F S+IG APP++ +L GW+M FV++G G+ V W + Sbjct: 124 TEWFPAKERSVAIGWFNIGSSIGALCAPPLVVWTILHGGWKMSFVVVGALGVIWFVLWML 183 Query: 189 FYRN-RNEVELTPSEILYLADGTDNDPNANAKLTWHEWKGLFKYRTTWGMLFGFMGVIYM 247 FY++ R++ L+P E Y+ +G + P K+ W + + R W + Sbjct: 184 FYKSPRDQKLLSPEERAYILEGQEKSPE---KVQRESWTKIVRSRNFWSIAIPRFLSEPA 240 Query: 248 VWLYLTWLPAYLEHERHLSIARTGWVVVIPYIFGTLGMLSSGYIADGLMKR-GMAPIRSR 306 + W+P Y+ ERH++I +P++ +G + GY++ K ++ SR Sbjct: 241 WQTFNAWIPLYMATERHMNIKEIAMFAWLPFLAADIGCVLGGYLSPLFHKHLKVSLFTSR 300 Query: 307 KWPICAGLIGAGAFTIPAA--YTPSTTMAIVYISLAMAFVNMASGGAWALVSVAIPRRLV 364 K L+G+ + PA + S +AI +S+ SG +++ S + V Sbjct: 301 K---LVMLVGSLSMIGPACVGFVDSPYVAIALLSIGGFAHQTLSGALYSITSDVFTKNQV 357 Query: 365 ASLGSLQNFGGYLGGSLAPLITGIVVDK 392 A+ L GYLG +L L+ GI+V + Sbjct: 358 ATATGLTGMSGYLGATLFTLLFGILVTQ 385 >gi|300313744|ref|YP_003777836.1| D-glucarate permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 129 bits (324), Expect = 6e-31, Method: Compositional matrix adjust. Identities = 93/325 (28%), Positives = 147/325 (45%), Gaps = 29/325 (8%) Query: 3 VQKSPKLKRIQIVALTFLMLAGIVNYLDRSTLSIANHSVSQELGLSASEMGLLLSAFSLA 62 +Q + K + V + + V DR+ L +A + +E +S +E G L S F A Sbjct: 6 IQHTKKKTHFRWVVGALIFIVYTVAAADRANLGVALPFLRKEFVMSNTEAGALASIFLFA 65 Query: 63 YAFSQLPIGAMLDKFGARVMLGAGMMVWSVAQLAGGFVGTLHQFLVARVF---LGLGEAP 119 Y+ +QLP G +FG + M+ SV G +GT L ++F LGL E P Sbjct: 66 YSLAQLPSGLAYTRFGIPRVFSLAMLATSVFT---GLIGTASSLLALKIFRFGLGLAEGP 122 Query: 120 QFPAG-AKVVSEWFAVRERGGPTGIFVASSTIGPALAPPILTVLMLSFGWRMMFVIMGIA 178 P G ++ WF RE+G TGIF+A++ GP L PP+ V+M +GWR +F + Sbjct: 123 -LPIGITSTINNWFPAREKGTMTGIFLAAAKFGPVLVPPVCAVIMYYWGWRHIFYFFAVP 181 Query: 179 GIAVAVGWYIFYRNR--NEVELTPSEILYLAD------GTDNDPNANAKL---------- 220 G+ ++V W + +NR ++ E+ ++ D G D DP Sbjct: 182 GVILSVVWLMVVKNRPSESPHVSAEELEHINDSQGAAAGQDADPAVTRDFALLDRIIRRS 241 Query: 221 ---TWHEWKGLFKYRTTWGMLFGFMGVIYMVWLYLTWLPAYLEHERHLSIARTGWVVVIP 277 T KG+F G G+ + +V + L W+P YL + SIA G V P Sbjct: 242 KVATIETTKGVFCSWNIMGAALGYFFMTAIVNVLLAWIPTYLISVKGYSIANMGMVAAAP 301 Query: 278 YIFGTLGMLSSGYIADGLMKRGMAP 302 ++ +G L G + D +++ P Sbjct: 302 WVGAVIGNLLGGAMTDRWLEKRRKP 326 >gi|300313393|ref|YP_003777485.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 124 bits (312), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 97/326 (29%), Positives = 157/326 (48%), Gaps = 15/326 (4%) Query: 4 QKSPKLKRIQIVALTFLMLAGIVNYLDRSTLSIANHSVSQELGLSASEMGLLLSAFSLAY 63 K+ K R I+ L + L I NYL R++L + ++ ELG+S + +++AF + Y Sbjct: 1 MKTIKGLRWWIIVL--VCLGTITNYLARNSLGVLAPTLKDELGMSTQQYSYVVAAFQVGY 58 Query: 64 AFSQLPIGAMLDKFGARVMLGAGMMVWSVA-QLAGGFVG--TLHQFLVARVFLGLGEAPQ 120 Q G ++D G R+ ++WSVA L G G +L F R +GL EA Sbjct: 59 TIMQPVCGFIVDLIGLRIGFALFGVLWSVAGVLHAGATGWLSLAGF---RALMGLTEAAA 115 Query: 121 FPAGAKVVSEWFAVRERGGPTGIFVASSTIGPALAPPILTVLMLSFGWRMMFVIMGIAGI 180 P+G K V+EWF +E+ G F + +++G LAPP++ L + +GW+ FV+ GI G Sbjct: 116 IPSGMKAVAEWFPDKEKSVAVGWFNSGTSLGAMLAPPLVIFLQIRYGWQSAFVVTGILGF 175 Query: 181 AVAVGWYIFYRN-RNEVELTPSEILYLADGTDNDPNA-NAKLTWHEWKGLFKYRTTWGML 238 A W+ FYR+ R+ L+ SE + G +A K K + + R W + Sbjct: 176 VWAALWFAFYRSPRDHAALSDSERETIISGQLRPVSAVRGK---RPVKDVVRSRRFWAIA 232 Query: 239 FGFMGVIYMVWLYLTWLPAYLEHERHLSIARTGWVVVIPYIFGTLGMLSSGYIADGLMKR 298 + W+P Y +ERHL + +P++ LG L+ GY++ L+ R Sbjct: 233 LARFFAEPAWQTFSFWIPLYFVNERHLDLKSIAMFAWLPFLAADLGGLAGGYLSPFLINR 292 Query: 299 GMAPIRSRKWPICAGLIGAGAFTIPA 324 P+ + +C ++GA PA Sbjct: 293 FRIPLVWSR--VCGVVLGAVMMIGPA 316 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.137 0.435 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,766,805 Number of extensions: 73366 Number of successful extensions: 473 Number of sequences better than 1.0e-08: 30 Number of HSP's gapped: 435 Number of HSP's successfully gapped: 30 Length of query: 578 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 488 Effective length of database: 1,193,720 Effective search space: 582535360 Effective search space used: 582535360 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)