BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0222.0020.p
         (325 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310774|ref|YP_003774866.1| anaerobic formate dehydrogenase...   215   3e-57
gi|300311781|ref|YP_003775873.1| hypothetical protein Hsero_2466...   182   3e-47
gi|300311015|ref|YP_003775107.1| anaerobic (selenocysteine-conta...   142   3e-35

>gi|300310774|ref|YP_003774866.1| anaerobic formate dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 790

 Score =  215 bits (548), Expect = 3e-57,   Method: Compositional matrix adjust.
 Identities = 102/142 (71%), Positives = 113/142 (79%)

Query: 164 KWKTGNGKANFITPDSLDEDPDMPADGPGVLRLMTTRGDSQFNTTVYGLDDRFRGVHGTR 223
           +WKT NG+ANF+TPD  D DPDMP D P VLRLMTTRGDSQFNTTVY LDDRFRGV GTR
Sbjct: 639 QWKTPNGRANFMTPDGFDPDPDMPGDAPEVLRLMTTRGDSQFNTTVYSLDDRFRGVWGTR 698

Query: 224 HVLLMNVADIGRLGLKEGDLVTASTVSQDGIARSVDDLRVHAFDIPAGCIMGYFPELNPL 283
            VLLMN+ D+ + GL  GD V A TVS DG+ R V  LRV  FDIP GC+MGYFPELN L
Sbjct: 699 RVLLMNLRDLQQHGLAAGDFVCAETVSADGVERRVPHLRVEPFDIPVGCVMGYFPELNRL 758

Query: 284 IPLAHHAKDSKVPAAKSIPVRL 305
           IPL HHA+ SKVPAAKS+P+RL
Sbjct: 759 IPLFHHARGSKVPAAKSVPIRL 780


>gi|300311781|ref|YP_003775873.1| hypothetical protein Hsero_2466
           [Herbaspirillum seropedicae SmR1]
          Length = 370

 Score =  182 bits (463), Expect = 3e-47,   Method: Compositional matrix adjust.
 Identities = 88/154 (57%), Positives = 111/154 (72%), Gaps = 16/154 (10%)

Query: 1   MLGYQPEHQIMSSFTPADQAEVQADAPVDAPADLPADAAIESSTPARWRSVNPLNAGKLF 60
           MLGYQPE Q ++SF+            ++ PA   A++  E   P RWRSV P N G++F
Sbjct: 26  MLGYQPELQSITSFSA-----------LETPA---AESGAEPG-PGRWRSVRPENEGRVF 70

Query: 61  RQCRNYAQEKVCNWMLDDQDAHALCASCRLTAVIPSLDDAGNRILWQRLEVAKRRLLHSL 120
           +QC NY +E +CNWMLD+Q+ H LCASCRLT VIP+LD+  NR+LW RLE AKRRLLH+L
Sbjct: 71  KQCLNYWRENICNWMLDEQETHELCASCRLTRVIPTLDEGNNRVLWSRLETAKRRLLHTL 130

Query: 121 WTMRLQPPPKPDDADNGLSFEFLQDMPH-ERVMT 153
           WT+RLQP PK +D  +GL+FEFLQD PH ERV+T
Sbjct: 131 WTLRLQPAPKMEDEQSGLAFEFLQDQPHGERVLT 164


>gi|300311015|ref|YP_003775107.1| anaerobic
           (selenocysteine-containing) formate dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 775

 Score =  142 bits (359), Expect = 3e-35,   Method: Compositional matrix adjust.
 Identities = 70/150 (46%), Positives = 97/150 (64%), Gaps = 3/150 (2%)

Query: 165 WKTGNGKANFITPDSLDEDPDMPA---DGPGVLRLMTTRGDSQFNTTVYGLDDRFRGVHG 221
           WKT +GKANFI  D   + P   A    G  +L LMTTR   Q+NTT+YG+DDR+RGV G
Sbjct: 622 WKTASGKANFIVHDIPKDTPIHRARAIHGEKLLTLMTTRSHDQYNTTIYGMDDRYRGVFG 681

Query: 222 TRHVLLMNVADIGRLGLKEGDLVTASTVSQDGIARSVDDLRVHAFDIPAGCIMGYFPELN 281
            R V+ +N AD+  LGLK+G+ V  ++V  DG+ R  +   +  ++IP GCI  Y+PE N
Sbjct: 682 QRRVVFINPADLQMLGLKDGEWVDITSVWDDGVERRAEGFLLVEYEIPRGCIGSYYPETN 741

Query: 282 PLIPLAHHAKDSKVPAAKSIPVRLTRTQMQ 311
           PL+PL   A  ++ P +KSIPV L+R+ +Q
Sbjct: 742 PLVPLESFADKARTPTSKSIPVLLSRSAVQ 771


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.319    0.135    0.418 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,075,121
Number of extensions: 46043
Number of successful extensions: 111
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 109
Number of HSP's successfully gapped: 3
Length of query: 325
Length of database: 1,619,870
Length adjustment: 85
Effective length of query: 240
Effective length of database: 1,217,395
Effective search space: 292174800
Effective search space used: 292174800
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 131 (55.1 bits)