BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0222.0018.p (502 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312843|ref|YP_003776935.1| response regulator containing a... 352 4e-98 gi|300310865|ref|YP_003774957.1| response regulator containing a... 344 9e-96 gi|300313918|ref|YP_003778010.1| transmembrane protein [Herbaspi... 244 1e-65 gi|300313329|ref|YP_003777421.1| transmembrane protein [Herbaspi... 238 1e-63 gi|300313993|ref|YP_003778085.1| diguanylate cyclase/phosphodies... 174 2e-44 >gi|300312843|ref|YP_003776935.1| response regulator containing a CheY-like receiver and a GGDEF domains protein [Herbaspirillum seropedicae SmR1] Length = 525 Score = 352 bits (903), Expect = 4e-98, Method: Compositional matrix adjust. Identities = 182/443 (41%), Positives = 271/443 (61%) Query: 1 LVVLACALLVVLAVVTDWRARKQQLQEAGATSHNIALALARHADDTFKQADTTLIFLAER 60 VVL CA + + AR QL+ + S N+A ALARHA D + D L+ +++R Sbjct: 23 FVVLICAAIAGGHLWQSMTARGVQLENNRSYSGNVAKALARHAYDVLQATDGLLLDMSDR 82 Query: 61 LRHDERKPASLRELEVFLAQQTVELPQLHGLFVIDKDGKWLLNSRQYQAGHVNSRDREYY 120 + A L +L L + + E+PQL GL+V D+ GK LL S N+ DR+Y+ Sbjct: 83 IDSVGIAHARLVDLMPMLRRLSSEMPQLDGLYVFDEHGKRLLTSSAPPLAPANNSDRDYF 142 Query: 121 LYHRQHEDLSPHISMPVQSRANGEWVVTISRRLNGADGSFDGVVMAAIRLAYFSKFYENF 180 ++HR H+D+ PH+ + SR+ G W++ +SRRLN DGSF GV++A + + +F++ Y Sbjct: 143 IWHRDHDDVGPHVGKALLSRSTGRWIIPMSRRLNHPDGSFAGVLLATLDINHFNQLYRTV 202 Query: 181 DIGEGGAVILAGNEGTLLVRRPLRNDSIGKSLAQAAIFRDHAAINASGSARIRSSQDGVI 240 +IG+GG++ L +GTLL R P I + +Q +F + + G + S D Sbjct: 203 EIGKGGSIALLLRDGTLLTRMPFDPGYINRDFSQGRLFLEGLPHSPFGYLEVVSPLDARQ 262 Query: 241 RINSYRHLDRYPLFVAAALSEQEVLASWRSDVLLHVSGLIVLLSLLVWLGGRLIRQIELR 300 R SY+ +D+YPL + L+ +E LA W+ ++ + +++LL+++ G RL+ QIELR Sbjct: 263 RFLSYQAVDKYPLVLVVTLAREEALADWKQQTFVYGAAVLLLLAIIGVFGWRLVGQIELR 322 Query: 301 VEAENAANHAREHVEFLNRTLQNLATQDGLTGLGNRRCFDEEMVRALRRCAREGKPLALI 360 + AE+ A A ++ N L+ LA QDGLTGL NRR D + +R R G LALI Sbjct: 323 LRAEDRALQAMGELQMANHRLEMLAHQDGLTGLANRRHLDTMLETEFKRAERSGAALALI 382 Query: 361 MLDVDFFKRYNDTYGHLAGDECLRKLSQAIRQGQKREGDLAARYGGEELVLMLPDCDGGN 420 M+DVDFFK+YND YGH AGDECLR+++ +++ Q+R GDLAARYGGEE+ ++LP D Sbjct: 383 MIDVDFFKQYNDLYGHQAGDECLRRVAGVLKERQRRPGDLAARYGGEEMAMLLPGTDAEG 442 Query: 421 AVAIAEETLARIRALQLPHQGNP 443 A A+AE+ A I+AL+LPH+GNP Sbjct: 443 ACAVAEKLRAGIQALELPHRGNP 465 >gi|300310865|ref|YP_003774957.1| response regulator containing a CheY-like receiver domain and a GGDEF domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 554 Score = 344 bits (883), Expect = 9e-96, Method: Compositional matrix adjust. Identities = 185/472 (39%), Positives = 282/472 (59%), Gaps = 1/472 (0%) Query: 16 TDWRARKQQLQEAGATSHNIALALARHADDTFKQADTTLIFLAERLRHDERKPASLRELE 75 T WRAR+ L E+ + N+ALAL +HA+ + K+AD L + E L H++R ++ Sbjct: 44 TAWRAREVALSESTTDASNVALALIQHAEQSIKEADIVLETVQEHLDHEDRSASARIRTH 103 Query: 76 VFLAQQTVELPQLHGLFVIDKDGKWLLNSRQYQAGHVNSRDREYYLYHRQHEDLSPHISM 135 F+ + LPQL L V+D+ G+ + +S+Q + +Y+ YH+ P I Sbjct: 104 DFMIRSVASLPQLAQLTVVDEQGRTVASSQQRMVQTGSPVQDDYFQYHQHRRSDRPFIGQ 163 Query: 136 PVQSRANGEWVVTISRRLNGADGSFDGVVMAAIRLAYFSKFYENFDIGEGGAVILAGNEG 195 P++S G+ +T+SRR+N DGSF GV++A I LAYF ++YE F+IG G ++ G Sbjct: 164 PMRSHVTGQRTLTVSRRINHVDGSFAGVIVAEIDLAYFQRYYEQFNIGPHGVILFGLTGG 223 Query: 196 TLLVRRPLRNDSIGKSLAQAAIFRDHAAINASGSARIRSSQDGVIRINSYRHLDRYPLFV 255 +L++RRPL D +S A + R H GS +RS +DGV RI+ ++ + YP+F Sbjct: 224 SLILRRPLLADMTDRSPAMSEALRKHMEQARRGSFAMRSERDGVERISCFQQGEAYPIFT 283 Query: 256 AAALSEQEVLASWRSDVLLHVSGLIVLLSLLVWLGGRLIRQIELRVEAENAANHAREHVE 315 AL++ ++L W V + G+++LL+++ + G RL+ + R AE A AR+ +E Sbjct: 284 VVALAKDDILEDWSRHVWVRGIGVVLLLAVICFGGKRLVVLVAGRERAEAQAIAARQELE 343 Query: 316 FLNRTLQNLATQDGLTGLGNRRCFDEEMVRALRRCAREGKPLALIMLDVDFFKRYNDTYG 375 L RTL+ + +DGLT + NRR FD + L R R G+ L+L+M+DVD FK++NDTYG Sbjct: 344 SLYRTLEAQSQKDGLTDVFNRRYFDAALAAELARLNRTGESLSLMMIDVDHFKQFNDTYG 403 Query: 376 HLAGDECLRKLSQAIRQGQKREGDLAARYGGEELVLMLPDCDGGNAVAIAEETLARIRAL 435 H AGD CLR+++ AI +R+GD+ ARYGGEE ++LP+C G+A +A + +R L Sbjct: 404 HAAGDVCLRQVAAAISTVARRQGDIVARYGGEEFAVILPNCHPGSAQGLAHRVVQAVRGL 463 Query: 436 QLPHQGNPNGIVTVSAGVAVLDPVSEDDV-AESLIGQADKALYLAKSTGRDR 486 ++ H+ +P G+VT+S GVA L + LI AD ALY AK GRDR Sbjct: 464 EIAHERSPMGVVTISVGVASLASGKSLLIEPRELIDSADGALYRAKEEGRDR 515 >gi|300313918|ref|YP_003778010.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 244 bits (623), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 165/502 (32%), Positives = 255/502 (50%), Gaps = 31/502 (6%) Query: 1 LVVLACALLVVLAVV--TDWRARKQQLQEAGATSHNIALALARHADDTFKQADTTLIFLA 58 +V+LA A+L+V+A+ T +++ +Q Q A + + N+ L R + +L LA Sbjct: 30 VVILAIAVLMVMAISASTLYQSYQQTRQSAVSNASNVTLVSQRAISRNLEMLSLSLDTLA 89 Query: 59 ERLRHDERKPASLRELE-----VFLAQQTVELPQLHGLFVIDKDGKWLLNSRQYQA-GHV 112 R +H P S R L +L T + + + V+D DG+ ++SR + G Sbjct: 90 YRFQH----PLSARRLNDAQRYAYLFGSTTNVSHIAVMAVLDADGRVQVSSRLGRGNGAP 145 Query: 113 NSRDREYYLYHRQHEDLSPHISMPVQSRANGEW-VVTISRRLNGADGSFDGVVMAAIRLA 171 + DR Y++ HR + ++S P+Q+ E VV +S+RL+ DGSF GVV+ A+ LA Sbjct: 146 HYADRAYFIAHRDSPTVGLYVSRPMQASLGNELQVVVLSKRLSNEDGSFAGVVVMALDLA 205 Query: 172 YFSKFYENFDIGEGGAVILAGNEGTLLVRRPLRNDSIGKSLAQAAIFRDHAAI--NASGS 229 YF + +E +G+ G + L +EG +R P R IG+ ++Q F+ A+ GS Sbjct: 206 YFRELFEGLALGDEGIISLYSDEGIAYMRVPYRETIIGRDISQTGNFQRLRAMLSEEEGS 265 Query: 230 ARIRSSQDGVIRINSYRHLDRYPLFVAAALSEQEVLASWRSDVLLHVSGLIVLLSLLVWL 289 ++ DGV R+ ++R + PL V +E+ + +WR + L + L WL Sbjct: 266 FFAHATNDGVRRLYTFRRIPGSPLIVFVGYAEEAIFKTWRKTFYAVLVSLAIFGLLTAWL 325 Query: 290 GGRLIRQIELRVEAENAANHAREHVEFLNRTLQNLATQDGLTGLGNRRCFDEEMVRALRR 349 + +++ RV AE R L+ LA DGLT L NRR DE + R Sbjct: 326 LLHVRQELRRRVTAE--------------RQLEALARTDGLTDLLNRRALDEALQATWER 371 Query: 350 CARE-GKPLALIMLDVDFFKRYNDTYGHLAGDECLRKLSQAIRQGQKREGDLAARYGGEE 408 C R G +++ +DVD+FK YNDTYGH GD L++++ + R D A RYGGEE Sbjct: 372 CQRNLGASFSVLFIDVDYFKAYNDTYGHKLGDTVLKEVAATLTARLPRHTDCAGRYGGEE 431 Query: 409 LVLMLPDCDGGNAVAIAEETLARIRALQLPHQGNPNGIVTVSAGVAVLDPVSEDDVAESL 468 V++L D A +A+ +R + H +P +VT+S GVA L+ V E + Sbjct: 432 FVVLLEQTDLQGAQMMAQRLCDAVRERHIAHTSSPLAVVTISVGVATLERDHHRRV-EDV 490 Query: 469 IGQADKALYLAKSTGRDRVCTS 490 + AD ALY AK GRDRV S Sbjct: 491 LNAADAALYRAKQAGRDRVHLS 512 >gi|300313329|ref|YP_003777421.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 501 Score = 238 bits (606), Expect = 1e-63, Method: Compositional matrix adjust. Identities = 159/499 (31%), Positives = 259/499 (51%), Gaps = 26/499 (5%) Query: 2 VVLACALLVVLAVV--TDWRARKQQLQEAGATSHNIALALARHADDTFKQADTTLIFLAE 59 ++L +++VL++ + + +Q A ++N+ L + R + + LA Sbjct: 10 LILGLIIMMVLSICGASLYEGYRQTRISAITEANNVTLIVQRAISRNMEILSLAMDTLAY 69 Query: 60 RLRHD-ERKPASLRELEVFLAQQTVELPQLHGLFVIDKDGKWLLNSRQYQAGHV--NSRD 116 R +H + AS E +L T + L ++D DG + SR+ + V N + Sbjct: 70 RYQHPLDLTHASDEERYNYLFGSTAAAHHITALAILDADGYVRVTSRRSASPSVVVNYSE 129 Query: 117 REYYLYHRQHEDLSPHISMPVQSRANGEW-VVTISRRLNGADGSFDGVVMAAIRLAYFSK 175 R Y+ HR D IS P+++ E VV +S+RL+ DGSFDGVV+ A+ ++YF Sbjct: 130 RTYFTVHRDSADAGLFISRPIKANLGNELPVVVLSKRLSRKDGSFDGVVLMALDMSYFRD 189 Query: 176 FYENFDIGEGGAVILAGNEGTLLVRRPLRNDSIGKSLAQAAIFRDHAA--INASGSARIR 233 + +GE G + L ++G +R P SIG+ + F+ A ++ G+ R Sbjct: 190 LFTGLTLGEDGIMSLYSDDGVTYMRVPYHPSSIGREITNNPNFQRLRAMLVDNDGNFFAR 249 Query: 234 SSQDGVIRINSYRHLDRYPLFVAAALSEQEVLASWRSDVLLHVSGLIVLLSLLVWLGGRL 293 + DGV R+ ++R + PL V SE+ + +W D L V L+++ + L + L Sbjct: 250 AHTDGVERLYTFRRIPDAPLIVLLGRSERFIFKTW-YDTLYSVLLLLIIFTALAGVLLML 308 Query: 294 IRQIEL-RVEAENAANHAREHVEFLNRTLQNLATQDGLTGLGNRRCFDEEMVRALRRCAR 352 +R+ L RV AE + L++LA DGLTGL NRR DE ++ +R R Sbjct: 309 VRRELLKRVAAE--------------KQLEDLARTDGLTGLLNRRSLDEALLAVWQRSQR 354 Query: 353 EGKP-LALIMLDVDFFKRYNDTYGHLAGDECLRKLSQAIRQGQKREGDLAARYGGEELVL 411 + + +DVD+FKRYNDTYGH GD L+ ++ +R+ +R+ DLA RYGGEE V+ Sbjct: 355 SPTAGFSTLFIDVDYFKRYNDTYGHEMGDTVLQAVAAVLREHLQRQNDLAGRYGGEEFVV 414 Query: 412 MLPDCDGGNAVAIAEETLARIRALQLPHQGNPNGIVTVSAGVAVLDPVSEDDVAESLIGQ 471 +L D A+ +A++ I +PH G+P G+++VS GVA ++ + E ++ Sbjct: 415 LLEQTDEHGALRMAQQLCEAIYNRGIPHSGSPLGVISVSIGVATMNRAPHQRM-EDVLNA 473 Query: 472 ADKALYLAKSTGRDRVCTS 490 AD ALY+AK GR+RVC + Sbjct: 474 ADHALYIAKREGRNRVCVA 492 >gi|300313993|ref|YP_003778085.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 573 Score = 174 bits (441), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 87/182 (47%), Positives = 118/182 (64%), Gaps = 1/182 (0%) Query: 313 HVEFLNRTLQNLATQDGLTGLGNRRCFDEEMVRALRRCAREGKPLALIMLDVDFFKRYND 372 +E NR L+ L+ DGLTG+ NRR FDE + +RR R G+ LAL+MLDVD+FK+YND Sbjct: 390 ELERANRKLEALSATDGLTGVANRRRFDEALANEMRRAVRSGQSLALLMLDVDYFKKYND 449 Query: 373 TYGHLAGDECLRKLSQAIRQGQKREGDLAARYGGEELVLMLPDCDGGNAVAIAEETLARI 432 YGHLAGDECLR +++ +++ +R DL ARYGGEE +++ +CD A+ AE I Sbjct: 450 RYGHLAGDECLRIVARVLQKSSRRATDLVARYGGEEFSVIIAECDTTQALQQAENICQAI 509 Query: 433 RALQLPHQGNPNGIVTVSAGVAVLDPVSEDDVAESLIGQADKALYLAKSTGRDRVCTSGA 492 L+LPH +P G ++ S GVAVL P E E ++ AD+ALY AK GR+RV Sbjct: 510 FELKLPHADSPFGYISASIGVAVLLP-DEHTAPEQMVRIADQALYHAKYQGRNRVVLGSD 568 Query: 493 ST 494 +T Sbjct: 569 AT 570 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,431,238 Number of extensions: 59354 Number of successful extensions: 273 Number of sequences better than 1.0e-08: 41 Number of HSP's gapped: 206 Number of HSP's successfully gapped: 42 Length of query: 502 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 413 Effective length of database: 1,198,455 Effective search space: 494961915 Effective search space used: 494961915 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)