BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0221.0052.p (143 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310012|ref|YP_003774104.1| outer membrane W protein [Herba... 222 8e-60 >gi|300310012|ref|YP_003774104.1| outer membrane W protein [Herbaspirillum seropedicae SmR1] Length = 245 Score = 222 bits (566), Expect = 8e-60, Method: Compositional matrix adjust. Identities = 112/143 (78%), Positives = 121/143 (84%), Gaps = 3/143 (2%) Query: 1 LAGLGELGTAKQWSPAIVAKYFFGDGTSKFRPFVGAGLTYVWYSDVKLSSAFQNRAALNT 60 L LGELG AKQWSPAIVAK++FG FRPFVGAG+TYVWYSDV LS AFQNRA+LN+ Sbjct: 106 LQALGELGKAKQWSPAIVAKWYFGGAQDAFRPFVGAGVTYVWYSDVSLSQAFQNRASLNS 165 Query: 61 GAAGGNASASLSSSWAPVLNVGATYNFNDRWSASLSVSYIPLKTDADITGNPGGAAAALG 120 G G A+A+LSSSWAPVL GATY FNDRWSAS SVSYIPLKTDADIT NP GA A LG Sbjct: 166 G---GTATANLSSSWAPVLTGGATYAFNDRWSASFSVSYIPLKTDADITANPAGAFAGLG 222 Query: 121 PQRYKTSITLDPIVSFLSVGYKF 143 PQRYKT+ITLDPIV+FLSVGYKF Sbjct: 223 PQRYKTTITLDPIVTFLSVGYKF 245 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.131 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 342,776 Number of extensions: 9626 Number of successful extensions: 30 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 30 Number of HSP's successfully gapped: 1 Length of query: 143 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 66 Effective length of database: 1,255,275 Effective search space: 82848150 Effective search space used: 82848150 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 126 (53.1 bits)