BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0221.0022.p
         (716 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310040|ref|YP_003774132.1| organic solvent tolerance trans...   906   0.0  

>gi|300310040|ref|YP_003774132.1| organic solvent tolerance
           transmembrane protein [Herbaspirillum seropedicae SmR1]
          Length = 766

 Score =  906 bits (2342), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 445/622 (71%), Positives = 506/622 (81%), Gaps = 19/622 (3%)

Query: 20  VLPISAQAQTTQNTP--AKTDDKDAPVNVSAEQMTGRPDRQINLDRDVEVVRGETTIKAD 77
           +LP+S  AQ T   P  A   D+DAPVNVSAEQMTGRPDRQ+NLDRDVEVV+G TTIK+D
Sbjct: 33  LLPLSVYAQMTLPAPVAAPAQDQDAPVNVSAEQMTGRPDRQVNLDRDVEVVKGPTTIKSD 92

Query: 78  KAEYRIIKDEVEATGHVWMKRLQDRYTGDKALMNMDSGKGYVTNPTYLFGKNGGRGKAEQ 137
           KAEYRII+DEVEATGHVWM+RLQD+YTGD A MNM +G GYV++PTYL GKNGGRGKAE+
Sbjct: 93  KAEYRIIQDEVEATGHVWMRRLQDQYTGDHAEMNMGTGAGYVSSPTYLIGKNGGRGKAER 152

Query: 138 IDFLSDDQAKVTEGTYSTCEALDPDWYIQASTMDLDSGRDVGTMHGGIVYFKGVPILGAP 197
           IDF+SDDQA VT+GTYSTCEA DPDWY++A+TMDLDSGRDVGT+ GG++YFK VPIL AP
Sbjct: 153 IDFISDDQALVTQGTYSTCEAPDPDWYLRANTMDLDSGRDVGTVRGGLLYFKDVPILAAP 212

Query: 198 LMSFPLSGERKSGVLPPTFGVTSTGGAELTVPYYFNIAPNRDLTLYPRYIARRGLQLGVE 257
            MSF LSGERKSGVLPPT G TSTGG EL VPYYFN+APN DLTL P+YIARRGLQ+G E
Sbjct: 213 WMSFALSGERKSGVLPPTIGATSTGGFELAVPYYFNLAPNYDLTLVPKYIARRGLQMGAE 272

Query: 258 GRYLGEDYSGTTKVEGIQDRVTGTGRYSLSSLHSQTLAPNLVFGWNVNKASDNDYPSDFS 317
            RY+GE Y+G    EGIQDRVTGTGRYSLSS HSQ LAP L   WN+NKASDNDY SDFS
Sbjct: 273 TRYMGESYNGVFNAEGIQDRVTGTGRYSLSSRHSQILAPGLQLDWNLNKASDNDYASDFS 332

Query: 318 HSVTASTQRLLTRDVSLVYSGTYWSAVGRMTKYQLLQDINNPIQKPYDRVPQLTLTAARQ 377
           HS+T ST RLL R  SL YS TYW+ VG+++KYQLLQD+N PI KPYDRVPQ+T    R 
Sbjct: 333 HSITGSTTRLLPRTASLTYSSTYWTLVGQVSKYQLLQDVNAPIVKPYDRVPQITFNGTRY 392

Query: 378 DVGGFDFNFASEFARFSNTYTLGSSAGSEMVGGDRMYANQSVSYPIIRPGYFITPKASLD 437
           D+GGFDFN  ++F RFS+           +VGGDR Y  QSVSYPI+RPGYFITPK +LD
Sbjct: 393 DIGGFDFNTVADFTRFSS---------GALVGGDRAYLTQSVSYPIVRPGYFITPKVTLD 443

Query: 438 ATTYRLNNVSVGAPTNLTRVVPTYSLDAGMTFERDTNLFGRDMTQTLEPRLFYVRTPYRD 497
           AT+Y+LNN++ G  TNLTR VPT SLD+G+ FER+++L GR MTQTLEPRLFYVRTPYRD
Sbjct: 444 ATSYKLNNLAPGQQTNLTRTVPTLSLDSGLIFERESDLLGRSMTQTLEPRLFYVRTPYRD 503

Query: 498 QNQYPNFDSGLADFNYAQIFSENRFVGHDRISDANQITGALVSRFIEQDGLERLRAAIGQ 557
           Q + P FDSGLADFN+AQIFSENRFVGHDRISDANQ+T A+ SR IE++GLERLRA +GQ
Sbjct: 504 QTKLPIFDSGLADFNFAQIFSENRFVGHDRISDANQLTAAVTSRLIEENGLERLRATVGQ 563

Query: 558 RFYFADPKV--------TLDGTSATVNGSKSDLLLSLGGQITRQLSLDNTVQYSETLRQM 609
           R+YFADPKV            TS+   GSKSDLLL+LGGQITR+ S DNTVQYSETLRQM
Sbjct: 564 RYYFADPKVDLTSTSTTATTTTSSGSLGSKSDLLLALGGQITREFSTDNTVQYSETLRQM 623

Query: 610 VRSNFGARWQPAPKXSSNFGAR 631
           VRSNFG RWQPAPK   N   R
Sbjct: 624 VRSNFGIRWQPAPKRVLNLQYR 645


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.318    0.134    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 2,215,047
Number of extensions: 97153
Number of successful extensions: 217
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 214
Number of HSP's successfully gapped: 1
Length of query: 716
Length of database: 1,619,870
Length adjustment: 92
Effective length of query: 624
Effective length of database: 1,184,250
Effective search space: 738972000
Effective search space used: 738972000
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 134 (56.2 bits)