BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0221.0021.p
         (282 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310040|ref|YP_003774132.1| organic solvent tolerance trans...   437   e-124

>gi|300310040|ref|YP_003774132.1| organic solvent tolerance
           transmembrane protein [Herbaspirillum seropedicae SmR1]
          Length = 766

 Score =  437 bits (1124), Expect = e-124,   Method: Compositional matrix adjust.
 Identities = 216/291 (74%), Positives = 247/291 (84%), Gaps = 14/291 (4%)

Query: 1   LDAGMTFERDTNLFGRDMTQTLEPRLFYVRTPYRDQNQYPNFDSGLADFNYAQIFSENRF 60
           LD+G+ FER+++L GR MTQTLEPRLFYVRTPYRDQ + P FDSGLADFN+AQIFSENRF
Sbjct: 469 LDSGLIFERESDLLGRSMTQTLEPRLFYVRTPYRDQTKLPIFDSGLADFNFAQIFSENRF 528

Query: 61  VGHDRISDANQITGALVSRFIEQDGLERLRAAIGQRFYFADPKV--------TLDGTSAT 112
           VGHDRISDANQ+T A+ SR IE++GLERLRA +GQR+YFADPKV            TS+ 
Sbjct: 529 VGHDRISDANQLTAAVTSRLIEENGLERLRATVGQRYYFADPKVDLTSTSTTATTTTSSG 588

Query: 113 VNGSKSDLLLSLGGQITRQLSLDNTVQYSETLRQMVRSNFGARWQPAPKKVLNLTYRLDR 172
             GSKSDLLL+LGGQITR+ S DNTVQYSETLRQMVRSNFG RWQPAPK+VLNL YRLDR
Sbjct: 589 SLGSKSDLLLALGGQITREFSTDNTVQYSETLRQMVRSNFGIRWQPAPKRVLNLQYRLDR 648

Query: 173 TNA---TGI---LKQMDISGQWPVSQRWYAVSRINYSIPDKTVAEGLLGMEYKADCWVFR 226
           T     TG+   LKQ+D+SGQWP+SQR YAV RINYSIPD+TVAEGLLG+EYKADCWVFR
Sbjct: 649 TYVNPYTGVLDPLKQVDVSGQWPLSQRIYAVGRINYSIPDRTVAEGLLGLEYKADCWVFR 708

Query: 227 LVAQRIPTSSSKASTSFFIQLELNGFSKIGSNPLDALSASIPGYQIINKPD 277
           +VAQRIPTSS+KAST FF QLELNGFS+IGSNP+DA+ +S+PGYQ +N PD
Sbjct: 709 VVAQRIPTSSTKASTGFFFQLELNGFSQIGSNPMDAIKSSVPGYQKVNLPD 759


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.319    0.135    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 777,777
Number of extensions: 27911
Number of successful extensions: 54
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 52
Number of HSP's successfully gapped: 1
Length of query: 282
Length of database: 1,619,870
Length adjustment: 84
Effective length of query: 198
Effective length of database: 1,222,130
Effective search space: 241981740
Effective search space used: 241981740
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 130 (54.7 bits)