BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0221.0015.p (392 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310043|ref|YP_003774135.1| S-adenosylmethionine-6-N',N'-ad... 311 8e-86 gi|300310042|ref|YP_003774134.1| 4-hydroxythreonine-4-phosphate ... 114 2e-26 >gi|300310043|ref|YP_003774135.1| S-adenosylmethionine-6-N',N'-adenosyl (rRNA) dimethyltransferase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 311 bits (796), Expect = 8e-86, Method: Compositional matrix adjust. Identities = 149/176 (84%), Positives = 163/176 (92%), Gaps = 2/176 (1%) Query: 216 SLKFDFASIPVPAGRKLRVVGNLPYNISSPLMFRLAELAPLVQDQHFMLQKEVVERMVAD 275 +LKFDF++IPVP GRKLRVVGNLPYNISSPL+F LAE+AP VQDQHFMLQKEVVERMVA+ Sbjct: 85 ALKFDFSTIPVPEGRKLRVVGNLPYNISSPLLFHLAEIAPQVQDQHFMLQKEVVERMVAE 144 Query: 276 PGSKAFGRLSVMLQWRYHMELMFVVPPTAFDPPPRVDSAIVRMIPVAQPLECDQLLLEEV 335 PGSKA+GRLSVMLQWRYHMEL+FVVPPTAFDPPP+VDSAIVRMIP+AQPL C Q LLE+V Sbjct: 145 PGSKAYGRLSVMLQWRYHMELLFVVPPTAFDPPPKVDSAIVRMIPLAQPLACQQALLEQV 204 Query: 336 VTKAFSQRRKVIRNCVAGLLAEADLIAAGIDPQARPEAVPMEQFVALANVL--RQH 389 VTKAFSQRRKVIRNCVAGL +E +L GIDPQARPEAVPMEQFVALAN+L RQH Sbjct: 205 VTKAFSQRRKVIRNCVAGLFSEDELRQVGIDPQARPEAVPMEQFVALANLLAARQH 260 >gi|300310042|ref|YP_003774134.1| 4-hydroxythreonine-4-phosphate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 340 Score = 114 bits (284), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 53/77 (68%), Positives = 67/77 (87%) Query: 138 VLAITCGEPAGIGPEISIRAAWQLRAEVRGVLLGDAALLAQTAQVLDPAIALSALSVQAF 197 +LA+TCGEPAGIGPEIS+RAAW+LR EVR +LLGDAALLAQ+A +D + ++A+S+QA+ Sbjct: 5 ILALTCGEPAGIGPEISLRAAWELRHEVRCILLGDAALLAQSAYEIDAQMRVAAISIQAW 64 Query: 198 RNSGLPNFPQDRLIVID 214 RNSGLP+FP D L VID Sbjct: 65 RNSGLPHFPTDCLTVID 81 Score = 105 bits (263), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 54/70 (77%), Positives = 59/70 (84%) Query: 63 AVLQTLDVAVEWALKGQFDAIVTAPLQKSTINDAGVPFTGHTEYLAERTATPQVVMMLAG 122 AVLQTLD+AV+ AL G F AIVTAPLQKSTINDAGV FTGHTEYLAE T TPQVVMMLAG Sbjct: 100 AVLQTLDLAVQGALSGAFAAIVTAPLQKSTINDAGVAFTGHTEYLAEVTHTPQVVMMLAG 159 Query: 123 GDAPXPQVVM 132 G +P +V + Sbjct: 160 GQSPHLRVAL 169 Score = 82.4 bits (202), Expect = 6e-17, Method: Compositional matrix adjust. Identities = 42/48 (87%), Positives = 43/48 (89%) Query: 1 LRVALATTHLALKDVSAAITFDGLGRTLDIIHADLRDKFGIARPRILV 48 LRVALATTHLALKDV AAIT D L RTLDI+HADLR KFGIARPRILV Sbjct: 165 LRVALATTHLALKDVPAAITADSLARTLDILHADLRSKFGIARPRILV 212 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.137 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,199,719 Number of extensions: 49301 Number of successful extensions: 146 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 145 Number of HSP's successfully gapped: 4 Length of query: 392 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 305 Effective length of database: 1,207,925 Effective search space: 368417125 Effective search space used: 368417125 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 131 (55.1 bits)