BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0221.0004.p (176 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310053|ref|YP_003774145.1| apolipoprotein N-acyltransferas... 105 2e-24 >gi|300310053|ref|YP_003774145.1| apolipoprotein N-acyltransferase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 541 Score = 105 bits (262), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 50/78 (64%), Positives = 55/78 (70%), Gaps = 2/78 (2%) Query: 34 LFALLAGVANVLAFAPFNIWPVQILTLAWLFRQI--SRTSSFRRNALTGWLYGFGWAACG 91 L + AG NV AFAPF +WP+QIL LAWLF + +S R L GWLYGFGWAA G Sbjct: 29 LMPVAAGAINVFAFAPFKLWPLQILALAWLFHGVLAQPQASMRSQFLRGWLYGFGWAAAG 88 Query: 92 VHWLYISMHDYGGMAGWM 109 VHWLYISMHDYGGM WM Sbjct: 89 VHWLYISMHDYGGMPAWM 106 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.130 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 276,111 Number of extensions: 7776 Number of successful extensions: 39 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 38 Number of HSP's successfully gapped: 1 Length of query: 176 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 97 Effective length of database: 1,245,805 Effective search space: 120843085 Effective search space used: 120843085 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 127 (53.5 bits)