BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0221.0003.p (160 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310054|ref|YP_003774146.1| Mg2+ and Co2+ transporter prote... 163 7e-42 >gi|300310054|ref|YP_003774146.1| Mg2+ and Co2+ transporter protein [Herbaspirillum seropedicae SmR1] Length = 292 Score = 163 bits (412), Expect = 7e-42, Method: Compositional matrix adjust. Identities = 80/86 (93%), Positives = 84/86 (97%) Query: 1 LLEVLQDAHARNLIDADALSMIEGVFQVSDLAVRDIMVPRSQMDTIDISKPIETWMPDVL 60 LLEVL DAH RNLIDADALSMIEGVFQVSDLAVRDIMVPRSQMDTIDISKPIETWMP+VL Sbjct: 36 LLEVLHDAHERNLIDADALSMIEGVFQVSDLAVRDIMVPRSQMDTIDISKPIETWMPEVL 95 Query: 61 STSHSRFPAVEGERDKVVGVLLAKDL 86 +TSHSRFPAV+GERDKVVG+LLAKDL Sbjct: 96 ATSHSRFPAVDGERDKVVGILLAKDL 121 Score = 115 bits (288), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 54/71 (76%), Positives = 62/71 (87%) Query: 88 IAQFNETLETAFSDEDADTIGGLVANHLGRVPRKGDEFDIDGMRFEVLRADARQAHVLLV 147 I QFNE + + FSD+DADTIGGLVANH+GRVPRKGDEFDIDG+ FEVLRADARQ HVLLV Sbjct: 222 IEQFNEEVGSRFSDDDADTIGGLVANHVGRVPRKGDEFDIDGLHFEVLRADARQVHVLLV 281 Query: 148 EKLPDTPAAEE 158 +LP PA++E Sbjct: 282 SQLPPAPASDE 292 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 476,390 Number of extensions: 17910 Number of successful extensions: 54 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 54 Number of HSP's successfully gapped: 2 Length of query: 160 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 82 Effective length of database: 1,250,540 Effective search space: 102544280 Effective search space used: 102544280 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)