BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0220.0008.p
         (428 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310489|ref|YP_003774581.1| glucarate dehydratase [Herbaspi...   531   e-152
gi|300310475|ref|YP_003774567.1| mandelate racemase/muconate lac...   168   7e-43

>gi|300310489|ref|YP_003774581.1| glucarate dehydratase
           [Herbaspirillum seropedicae SmR1]
          Length = 455

 Score =  531 bits (1367), Expect = e-152,   Method: Compositional matrix adjust.
 Identities = 274/363 (75%), Positives = 293/363 (80%), Gaps = 25/363 (6%)

Query: 2   SNTSPLSNSSGSSAPVVTAMQVIPVAGRDGMLLNLSGAHGPYFTRNIVILTDSAGNTGVG 61
           S   P +      AP VT M+VIPVAG D MLLNLSGAHGPYFTRN+VILTDSAGNTGVG
Sbjct: 4   SAAIPHTVQPVQGAPRVTEMRVIPVAGHDSMLLNLSGAHGPYFTRNLVILTDSAGNTGVG 63

Query: 62  EVPGGERIRQTLEDACELVVGQSIANYHAVLNRMRQTFSDRDA----------------- 104
           EVPGGE IRQTLEDA  LVVGQSI N   +LNR R  F+DRD                  
Sbjct: 64  EVPGGEGIRQTLEDARTLVVGQSIGNVQGILNRARTAFADRDTGGRGLQTFDLRIAIHAV 123

Query: 105 --------XLLGKFLGVPVAALLGEGQQRDKVEMLGYLFYVGDRNATDLDYHSEPDNADD 156
                    LLGKFL VPVAALLGEGQQRD+VEMLGYLFYV DRN TDL Y + PD  DD
Sbjct: 124 TALEAALLDLLGKFLDVPVAALLGEGQQRDEVEMLGYLFYVADRNKTDLPYLAAPDAQDD 183

Query: 157 WQRLRHEKAMDADAIVRLAEAAYQRYGFNDFKLKGGVLRGEDEIEAVTALHERFPQARIT 216
           W RLRHE A+  +A+VRLAEAAY RYGFNDFKLKGGV+RGE+EI AVTAL ERFP+ARIT
Sbjct: 184 WTRLRHEAALTPEAVVRLAEAAYARYGFNDFKLKGGVMRGEEEIAAVTALAERFPKARIT 243

Query: 217 LDPNGGWLLKDAIRLCRDQHDVLAYAEDPCGAENGYSGREVMAEFRRATGLQTATNMIAT 276
           LDPNGGWLLKDAIRLCRDQHDVLAYAEDPCGAE+GYSGREVMAEFRRATGLQTATNM+AT
Sbjct: 244 LDPNGGWLLKDAIRLCRDQHDVLAYAEDPCGAEDGYSGREVMAEFRRATGLQTATNMVAT 303

Query: 277 DWREMGHAIQLQSVDIPLADPHFWTMQGSIRVAQLCDAWGLTWGSHSNNHFDISLAMFTQ 336
           DWR+MGHAI LQSVDIPLADPHFWTMQGS+RVAQ+C  WGLTWGSHSNNHFD+SLAMFT 
Sbjct: 304 DWRQMGHAISLQSVDIPLADPHFWTMQGSVRVAQMCHEWGLTWGSHSNNHFDVSLAMFTH 363

Query: 337 VAA 339
           VAA
Sbjct: 364 VAA 366


>gi|300310475|ref|YP_003774567.1| mandelate racemase/muconate
           lactonizing [Herbaspirillum seropedicae SmR1]
          Length = 424

 Score =  168 bits (426), Expect = 7e-43,   Method: Compositional matrix adjust.
 Identities = 118/353 (33%), Positives = 169/353 (47%), Gaps = 49/353 (13%)

Query: 18  VTAMQVIPVAGRDGMLLNLSGAHGPYFTRNIVILTDSAGNTGVGEVPGGERIRQTLEDAC 77
           +T + V P+A +DG LLN SG H PY  R+I+ +    G  G+GE  G     + L+   
Sbjct: 3   ITRVTVTPIAFKDGPLLNASGIHEPYALRSIIEIETDNGYIGLGESYGDAPALKILDLVK 62

Query: 78  ELVVGQSIANYHAVLNRMR---------------------------QTFSDRDAXLL--- 107
           E +VG    N + +   +R                             +S  +   L   
Sbjct: 63  ERLVGLDPFNLNGLRAIVRGVVAAMAPASNAGAELAPGSHASKAVSNAYSAFEVACLDAQ 122

Query: 108 GKFLGVPVAALLGEGQQRDKVEMLGYLFYVGDRNATDLDYHSEPDNADDWQRLRHEKAMD 167
            ++L VP+  LLG G  R ++    YLF+   + A  +D    P   D W      +A+ 
Sbjct: 123 ARYLNVPLVDLLG-GAVRKEIPFSAYLFF---KYAQHID---SPYAPDAWG-----EALS 170

Query: 168 ADAIVRLAEAAYQRYGFNDFKLKGGVLRGEDEIEAVTALHERFPQARITLDPNGGWLLKD 227
            + IV  A    +  GF   KLK G L  E E+  + AL + FP A + +DPNG W L+ 
Sbjct: 171 EEQIVAQARKMIEENGFQSIKLKAGALAPEHEVACIKALRKAFPDAPLRIDPNGNWSLQT 230

Query: 228 AIRLCRDQHDVLAYAEDPCGAENGYSGREVMAEFRRATGLQTATNMIATDWREMGHAIQL 287
           AIR+     D L Y EDP        G E MAE  R TGL  ATNM+ TD+ E+  ++ L
Sbjct: 231 AIRMAELLGDDLQYYEDPT------PGLEGMAELHRRTGLPLATNMVVTDFDELRRSVAL 284

Query: 288 QSVDIPLADPHFW-TMQGSIRVAQLCDAWGLTWGSHSNNHFDISLAMFTQVAA 339
            SV I LAD H+W  ++ + ++A++CD +GL    HSN+H  ISL   + VAA
Sbjct: 285 DSVQIVLADHHYWGGLRDTQQLAKMCDVFGLGVSMHSNSHLGISLMAMSHVAA 337


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.319    0.134    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,308,842
Number of extensions: 54975
Number of successful extensions: 144
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 141
Number of HSP's successfully gapped: 2
Length of query: 428
Length of database: 1,619,870
Length adjustment: 88
Effective length of query: 340
Effective length of database: 1,203,190
Effective search space: 409084600
Effective search space used: 409084600
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 132 (55.5 bits)