BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0220.0007.p (445 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310490|ref|YP_003774582.1| major facilitator superfamily p... 659 0.0 gi|300310476|ref|YP_003774568.1| major facilitator superfamily t... 630 0.0 gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 330 1e-91 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 322 4e-89 gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 295 5e-81 >gi|300310490|ref|YP_003774582.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 447 Score = 659 bits (1701), Expect = 0.0, Method: Compositional matrix adjust. Identities = 330/415 (79%), Positives = 375/415 (90%) Query: 25 LASAIDKVKWRILPLFVIMFIANYIDRVNIGFVRSHLATDLGIGSAAFGLGAGLFFIGYA 84 L SA+ K+KWRILPLFV MFI NYIDRVNIGFVRSHL+TDLGIG+AAFGLGAGLFF+GYA Sbjct: 14 LTSAVSKIKWRILPLFVAMFIINYIDRVNIGFVRSHLSTDLGIGAAAFGLGAGLFFVGYA 73 Query: 85 IFEVPSNMLLQKFGAKAWLTRIMLTWGVVATSMAFVHNETSFYILRFLLGVAEAGFFPGV 144 +FEVPSNMLLQ++GA+AWLTRIM TWG+VA MAFV +ETSFY +RFLLGVAEAGFFPGV Sbjct: 74 LFEVPSNMLLQRYGARAWLTRIMFTWGLVAACMAFVQSETSFYFMRFLLGVAEAGFFPGV 133 Query: 145 VYYFTQWLPNSERGKAMAVFLSGSAIASILSGPISGALLQIEGGGLHGWQWMFIIEGLAS 204 VYYFTQWLPN ERG+AMAVFLSGSAIASILSGPI+GALL IEGGGLHGWQWMFIIEG AS Sbjct: 134 VYYFTQWLPNHERGRAMAVFLSGSAIASILSGPITGALLLIEGGGLHGWQWMFIIEGGAS 193 Query: 205 VLLCFVVWRRLDSTPQDARWLSDDERHSLTTIIAHEQRERQASKPVHVSSWSLLKDPQIL 264 ++ C VVW LDSTP++A+WLS ++R +L +IA EQ +R A++P HVS+ SLL+DPQI+ Sbjct: 194 MVFCVVVWLVLDSTPREAKWLSQEQREALAAVIAAEQEQRAANRPAHVSALSLLRDPQIV 253 Query: 265 LFCFIYFAIQLTIYGATFWLPSIIKKMGHFNDFQVGLFNSIPWIISIVAMYIFASLAARF 324 LFCFIYFAIQLTIYGATFWLP+IIKKMGHFNDFQVGLFNSIPWI++I AMY+FA LAAR+ Sbjct: 254 LFCFIYFAIQLTIYGATFWLPTIIKKMGHFNDFQVGLFNSIPWIVAIAAMYLFAVLAARY 313 Query: 325 RHQQAWVAATLIIAAAGMFASTTGGPVFAFVAICFAGIGFKAASSLFWPIPQAYLDARIA 384 + Q WVA TL+I+AAGM S GPV FVA+CFAGIGFKAASSLFWPIPQAYLDARIA Sbjct: 314 KKPQFWVATTLVISAAGMAISAVAGPVIGFVAVCFAGIGFKAASSLFWPIPQAYLDARIA 373 Query: 385 AAVIALVNSIGNLGGFFAPATFGYLEQKTGSIQGGLYALAVASVIAAGLVFLART 439 AAV+AL+NS+GNLGGFFAPATFGYLEQKTGSIQGGL+AL+ AS++AA LVFLART Sbjct: 374 AAVLALINSLGNLGGFFAPATFGYLEQKTGSIQGGLFALSGASLLAAVLVFLART 428 >gi|300310476|ref|YP_003774568.1| major facilitator superfamily transporter protein [Herbaspirillum seropedicae SmR1] Length = 449 Score = 630 bits (1625), Expect = 0.0, Method: Compositional matrix adjust. Identities = 317/415 (76%), Positives = 365/415 (87%) Query: 25 LASAIDKVKWRILPLFVIMFIANYIDRVNIGFVRSHLATDLGIGSAAFGLGAGLFFIGYA 84 L A+ K+K R+LPLFVIMFI NYIDRVNIGFVRSHLATD+GIG+AA+GLGAGLFF+GYA Sbjct: 26 LQQAVAKIKQRVLPLFVIMFIVNYIDRVNIGFVRSHLATDVGIGTAAYGLGAGLFFVGYA 85 Query: 85 IFEVPSNMLLQKFGAKAWLTRIMLTWGVVATSMAFVHNETSFYILRFLLGVAEAGFFPGV 144 +FEVPSN+LLQ+FGAKAWLTRIM TWG+ AT+MAFV++ET+FY+LRFLLG AEAGFFPGV Sbjct: 86 LFEVPSNLLLQRFGAKAWLTRIMATWGLAATAMAFVNSETTFYVLRFLLGAAEAGFFPGV 145 Query: 145 VYYFTQWLPNSERGKAMAVFLSGSAIASILSGPISGALLQIEGGGLHGWQWMFIIEGLAS 204 +YYFTQWLP SERG+AMA+FLSGSA+ASILSGPISG LLQI+GGGL GWQWMFIIEG+AS Sbjct: 146 IYYFTQWLPASERGRAMAIFLSGSALASILSGPISGGLLQIDGGGLQGWQWMFIIEGMAS 205 Query: 205 VLLCFVVWRRLDSTPQDARWLSDDERHSLTTIIAHEQRERQASKPVHVSSWSLLKDPQIL 264 VLLC VW LDS P DA+WL+ ER ++T I EQ ER +P VS LL+DPQIL Sbjct: 206 VLLCGFVWFWLDSMPADAKWLTAQERSAITRAIVDEQAERMKHQPAQVSPRQLLRDPQIL 265 Query: 265 LFCFIYFAIQLTIYGATFWLPSIIKKMGHFNDFQVGLFNSIPWIISIVAMYIFASLAARF 324 +FCFIYF+I LTIYGATFWLPSII+KMG F+DFQVGLFNSIPW+ISIVAMY FA+LAARF Sbjct: 266 IFCFIYFSISLTIYGATFWLPSIIRKMGSFSDFQVGLFNSIPWLISIVAMYAFAALAARF 325 Query: 325 RHQQAWVAATLIIAAAGMFASTTGGPVFAFVAICFAGIGFKAASSLFWPIPQAYLDARIA 384 +HQQAW A L+IAAAGMFAST G V+AFV+ICFA +GFKAASSLFWP+PQAYLDARIA Sbjct: 326 KHQQAWAATALVIAAAGMFASTFGNSVYAFVSICFAAVGFKAASSLFWPLPQAYLDARIA 385 Query: 385 AAVIALVNSIGNLGGFFAPATFGYLEQKTGSIQGGLYALAVASVIAAGLVFLART 439 A VIAL+NSIGNLGGF AP FG+LEQ+TGSIQGGL LA+ S++AAG+VFLAR+ Sbjct: 386 AGVIALINSIGNLGGFVAPTAFGFLEQRTGSIQGGLMGLAITSLVAAGVVFLARS 440 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 330 bits (847), Expect = 1e-91, Method: Compositional matrix adjust. Identities = 171/411 (41%), Positives = 253/411 (61%), Gaps = 10/411 (2%) Query: 30 DKVKWRILPLFVIMFIANYIDRVNIGFVRSHLATDLGIGSAAFGLGAGLFFIGYAIFEVP 89 KV WR+LPL + ++A+Y+DRVN+GF + + DL +GLGAG+FF+GY +FEVP Sbjct: 22 SKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYFVFEVP 81 Query: 90 SNMLLQKFGAKAWLTRIMLTWGVVATSMAFVHNETSFYILRFLLGVAEAGFFPGVVYYFT 149 SNM+L K GA+ W+ RIM+TWG+++ +MA+V FYI+RFLLGVAEAGFFPGV+ Y T Sbjct: 82 SNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPGVILYLT 141 Query: 150 QWLPNSERGKAMAVFLSGSAIASILSGPISGALLQIEGG--GLHGWQWMFIIEGLASVLL 207 W P + RGK A+F++G A++ ++ GP+SG ++ G G GWQWMFI+E + S++L Sbjct: 142 YWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEAIPSLIL 201 Query: 208 CFVVWRRLDSTPQDARWLSDDERHSLTTIIAHEQRERQASKPVHVSSWSLLKDPQILLFC 267 +V + +DA WLS++E+ L I+HE+ +++ H+S ++ + ++ L Sbjct: 202 GVMVILYMKDRIRDADWLSEEEKRLLEANISHEESQKE-----HLSLGAMFANGRVWLGA 256 Query: 268 FIYFAIQLTIYGATFWLPSIIKKMGHFNDFQVGLFNSIPWIISIVAMYIFASLAARFRHQ 327 IYF + +YG +FWLP+IIK G + VGL +IP+ + AM + A R + Sbjct: 257 LIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSADARRER 316 Query: 328 QAWVAATLIIAAAGMFASTTGG--PVFAFVAICFAGIGFKAASSLFWPIPQAYLDARIAA 385 + VA I G+ ST G A ++ A +G LFW +P A+L AA Sbjct: 317 RWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLASVGILTTLPLFWSLPTAFLSGTAAA 376 Query: 386 AVIALVNSIGNLGGFFAPATFGYLEQKTGSIQGGLYALAVASVIAAGLVFL 436 A IAL+NS+GNL GF +P G+L+ T S G+Y LA AS++ GL+ L Sbjct: 377 AGIALINSLGNLAGFVSPFLVGWLKDATQSTNAGMYVLA-ASLVIGGLLTL 426 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 322 bits (825), Expect = 4e-89, Method: Compositional matrix adjust. Identities = 163/408 (39%), Positives = 250/408 (61%), Gaps = 14/408 (3%) Query: 31 KVKWRILPLFVIMFIANYIDRVNIGFVRSHLATDLGIGSAAFGLGAGLFFIGYAIFEVPS 90 KV WRI+P ++ ++ Y+DRVN+GF + ++ DLG FGLGAGLFFIGY +FEVPS Sbjct: 24 KVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQDLGFSETVFGLGAGLFFIGYFLFEVPS 83 Query: 91 NMLLQKFGAKAWLTRIMLTWGVVATSMAFVHNETSFYILRFLLGVAEAGFFPGVVYYFTQ 150 N+L+ K GA+ W+ RIM+TWG+++ + FV N T FYILRFLLG+AEAGF+PG++ Y T Sbjct: 84 NILMHKVGARIWIARIMITWGILSAAFMFVQNATQFYILRFLLGLAEAGFYPGIILYLTY 143 Query: 151 WLPNSERGKAMAVFLSGSAIASILSGPISGALLQI--EGGGLHGWQWMFIIEGLASVLLC 208 W P+ R K +AVF+SG +A IL P+SG ++ + GGL GWQWMF+IE + +VL+ Sbjct: 144 WFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFHQNGGLEGWQWMFLIEAIPAVLIG 203 Query: 209 FVVWRRLDSTPQDARWLSDDERHSLTTIIAHEQRERQASKPVHVSSWSLLKDPQILLFCF 268 LD+ + A+WL+D+E+ SL I + + +++ +++KD ++ L C Sbjct: 204 VATVLYLDNDVKSAKWLNDEEKASLQADIDGDAKGKESKHGFG----AIVKDARVWLMCL 259 Query: 269 IYFAIQLTIYGATFWLPSIIKKMGHFNDFQVGLFNSIPWIISIVAMYIFASLAARFRHQQ 328 IYF+ + YG T W+P+++K G + ++GL ++IP+ +I+AM + A R R ++ Sbjct: 260 IYFSFVMGQYGLTLWMPTLVKATGVKGNLEIGLLSAIPFGCAIIAMNLIGRSADRMRERR 319 Query: 329 AWVAATLIIAAAG-----MFASTTGGPVFAFVAICFAGIGFKAASSLFWPIPQAYLDARI 383 + ++ G +FA T + ++ A G + LFW +P A+L Sbjct: 320 WHLVIPALMGGVGFVGAALFADNTA---VSIASLSLAAAGVLTCAPLFWSLPTAFLSGAA 376 Query: 384 AAAVIALVNSIGNLGGFFAPATFGYLEQKTGSIQGGLYALAVASVIAA 431 AA IA +NS+GNL GF +P GYL+ T + G+Y LAV V+ + Sbjct: 377 AAVGIAAINSVGNLAGFVSPYLIGYLKDLTHNNATGMYMLAVMLVVGS 424 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 295 bits (755), Expect = 5e-81, Method: Compositional matrix adjust. Identities = 170/408 (41%), Positives = 246/408 (60%), Gaps = 8/408 (1%) Query: 31 KVKWRILPLFVIMFIANYIDRVNIGFVRSHLATDLGIGSAAFGLGAGLFFIGYAIFEVPS 90 K+ WR++P V +F+ +IDRVN+GF + + DL A +GLGAG+FFIGY IFEVPS Sbjct: 27 KIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFFIGYFIFEVPS 86 Query: 91 NMLLQKFGAKAWLTRIMLTWGVVATSMAFVHNETSFYILRFLLGVAEAGFFPGVVYYFTQ 150 N+LL+K GA+ L RI + WG+ + +MA+V TSFYILRFLLG+ EAGFFPGVV Y T Sbjct: 87 NLLLEKIGARKTLARITILWGLASMAMAYVTTPTSFYILRFLLGIFEAGFFPGVVLYLTY 146 Query: 151 WLPNSERGKAMAVFLSGSAIASILSGPISGALLQ-IEG-GGLHGWQWMFIIEGLASVLLC 208 W P R + +F++ AIA + GPI+G ++ +EG G L WQW+FI+EG+ S+L Sbjct: 147 WFPAQRRARVNGLFMTSFAIAGAVGGPIAGLIMSGMEGVGHLANWQWLFILEGIPSILAG 206 Query: 209 FVVWRRLDSTPQDARWLSDDERHSLTTIIAHEQRERQASKPVHVSSWSLLKDPQILLFCF 268 F V L P +A+WLS+ E++++T ++A E ++ H S + ++ + Sbjct: 207 FAVLAWLPERPANAKWLSEAEKNAVTQVLAAE----NSAPGKHFSFKDACTNYRVWICAA 262 Query: 269 IYFAIQLTIYGATFWLPSIIKKMGHFNDFQVGLFNSIPWIISIVAMYIFASLAARFRHQQ 328 +YF + FW PSIIK++G + Q+GL ++IP+I +AM + + ++ Sbjct: 263 VYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIPFIAGTLAMIWNGFHSDKTGERR 322 Query: 329 AWVAATLIIAAAGMFAS--TTGGPVFAFVAICFAGIGFKAASSLFWPIPQAYLDARIAAA 386 A +IA G+ A+ T G A ++ A IG AA +FW IP A+L AA Sbjct: 323 LHSAIATLIACIGLVATGMTLGNATLALCSLTLAAIGILAAFPVFWSIPGAFLAGTAAAG 382 Query: 387 VIALVNSIGNLGGFFAPATFGYLEQKTGSIQGGLYALAVASVIAAGLV 434 IAL+NSIGNL GF AP G L+ TGS+ GLY +A ++A+ LV Sbjct: 383 GIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVAALELVASFLV 430 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.139 0.437 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,344,370 Number of extensions: 53500 Number of successful extensions: 341 Number of sequences better than 1.0e-08: 21 Number of HSP's gapped: 310 Number of HSP's successfully gapped: 21 Length of query: 445 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 357 Effective length of database: 1,203,190 Effective search space: 429538830 Effective search space used: 429538830 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)