BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0216.0027.p
         (132 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310491|ref|YP_003774583.1| transmembrane protein [Herbaspi...   213   5e-57

>gi|300310491|ref|YP_003774583.1| transmembrane protein
           [Herbaspirillum seropedicae SmR1]
          Length = 307

 Score =  213 bits (541), Expect = 5e-57,   Method: Compositional matrix adjust.
 Identities = 98/131 (74%), Positives = 115/131 (87%)

Query: 1   MMLVLIGFAVFVASKWWQRRRFRKSLEMARISVGELDRLLKSGVRPTIVDVRSPASQQIG 60
           M+++ +  A FVA+KWWQRRRFRKSLEMARISV EL+ +L+ G  PTI+DVRSP SQQ G
Sbjct: 176 MLMIAVALAAFVANKWWQRRRFRKSLEMARISVDELNTMLEEGNAPTIIDVRSPLSQQDG 235

Query: 61  RIPGALTISNQEIQTFAFEAPMEDEVIVYCACPNEASAALVAKQLMRRGYKRVRPLTGGI 120
           RIPGA+TISNQEIQTF F+ P+E EV+VYCACPNEASAALVA+QLMRRGY RVRPLTGGI
Sbjct: 236 RIPGAVTISNQEIQTFVFDGPVEGEVVVYCACPNEASAALVARQLMRRGYTRVRPLTGGI 295

Query: 121 DAWVAAGHSIE 131
           DAW AAG++I+
Sbjct: 296 DAWRAAGYAID 306


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.324    0.135    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 397,226
Number of extensions: 13720
Number of successful extensions: 61
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 62
Number of HSP's successfully gapped: 1
Length of query: 132
Length of database: 1,619,870
Length adjustment: 75
Effective length of query: 57
Effective length of database: 1,264,745
Effective search space: 72090465
Effective search space used: 72090465
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 125 (52.8 bits)