BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0216.0024.p (96 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313800|ref|YP_003777892.1| transmembrane protein [Herbaspi... 96 8e-22 >gi|300313800|ref|YP_003777892.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 140 Score = 95.5 bits (236), Expect = 8e-22, Method: Compositional matrix adjust. Identities = 46/90 (51%), Positives = 64/90 (71%) Query: 4 IDDPLHVGRAFTLGFAIFAEVLCPLLIVPGILTRLASAAVLVLLGVSMFLVHPDWSVAEG 63 I+DP +G +L AIFAE LCPLLI G+ TRLA ++ +L V+M VHPDWS+AEG Sbjct: 51 IEDPFGLGPTVSLWAAIFAEALCPLLIAAGLFTRLACLPIIGVLLVAMLAVHPDWSIAEG 110 Query: 64 QFGWLLMIVFGSIALMGAGRYSIDAVLAKK 93 QFGWLL+++F +IAL G G++ + +A+ Sbjct: 111 QFGWLLLVIFTAIALCGPGQWRVGHGMAQP 140 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.330 0.145 0.444 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 268,010 Number of extensions: 7457 Number of successful extensions: 50 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 50 Number of HSP's successfully gapped: 1 Length of query: 96 Length of database: 1,619,870 Length adjustment: 65 Effective length of query: 31 Effective length of database: 1,312,095 Effective search space: 40674945 Effective search space used: 40674945 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 123 (52.0 bits)