BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0216.0020.p (88 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309642|ref|YP_003773734.1| aldo/keto reductase [Herbaspiri... 57 3e-10 >gi|300309642|ref|YP_003773734.1| aldo/keto reductase [Herbaspirillum seropedicae SmR1] Length = 280 Score = 57.0 bits (136), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 28/69 (40%), Positives = 45/69 (65%), Gaps = 1/69 (1%) Query: 2 PLAYGKVMTDPTLIGIAKAHQANPAQIALAWSLQQ-GFATIPSSTKRANLESNLAAQKIV 60 P+ G+++ P L +A+AH+ PAQIALAW L+Q G IP + A++++N A+ I Sbjct: 196 PIEQGRILKHPALTALAQAHRVTPAQIALAWVLRQPGVVAIPKAADVAHVQANRASLDIS 255 Query: 61 LSDADMQAI 69 LS AD++ + Sbjct: 256 LSAADLKIL 264 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.127 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 289,054 Number of extensions: 9252 Number of successful extensions: 31 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 32 Number of HSP's successfully gapped: 1 Length of query: 88 Length of database: 1,619,870 Length adjustment: 58 Effective length of query: 30 Effective length of database: 1,345,240 Effective search space: 40357200 Effective search space used: 40357200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 123 (52.0 bits)