BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0216.0019.p (263 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311178|ref|YP_003775270.1| lipase [Herbaspirillum seropedi... 295 4e-81 gi|300311659|ref|YP_003775751.1| esterase/lipase [Herbaspirillum... 135 3e-33 gi|300309659|ref|YP_003773751.1| esterase-lipase superfamily pro... 122 2e-29 >gi|300311178|ref|YP_003775270.1| lipase [Herbaspirillum seropedicae SmR1] Length = 340 Score = 295 bits (754), Expect = 4e-81, Method: Compositional matrix adjust. Identities = 142/249 (57%), Positives = 177/249 (71%), Gaps = 1/249 (0%) Query: 11 RIRILRPQGN-KETLPVVMYFHGAGWVMGDVNTHDRLVRQIVDGAKVAVVFVDYDRSPEA 69 ++ I+RP G+ K+TLP M+FHG GWV+GD TH+RLVR +V + VFV+Y SPEA Sbjct: 88 KLTIVRPAGSAKKTLPAFMFFHGGGWVLGDFPTHERLVRDLVANSGAVAVFVNYTPSPEA 147 Query: 70 RYPIAIEQDYAATKYIAEHAAEFNVDASRLAIAGDSVGGNMTAVVSLLAKERKGPAIKYQ 129 Y +AI Q YAATK++AEH E NVD RLA+AG+SVGGNM AVV+L+AK++ GPA++ + Sbjct: 148 HYGVAINQAYAATKWVAEHGKEINVDGKRLAVAGNSVGGNMAAVVALMAKDKGGPALRSE 207 Query: 130 VLFYPVTDTNFENGSYKQFANGPWLTKEAMKWFWNAYLPAGANRKDPHIAPLNATLEQLK 189 VL +PVTD NFE SY QFANG +LTK MKWFW++Y A RK+ +PLNAT QL Sbjct: 208 VLLWPVTDANFETASYNQFANGYFLTKNMMKWFWDSYTTDAAKRKEITASPLNATPAQLS 267 Query: 190 DLPPALVITDENDVLRDEGEAYAAKLAKAGVPVTQVRYLGTIHDFVMLNALGDTPAAKSA 249 LPP L+ T E DVLRDEGE Y KLA AGV VT VRY G IHDF +LN L PA ++A Sbjct: 268 GLPPTLIQTAEKDVLRDEGEEYGRKLAAAGVAVTSVRYNGMIHDFGLLNVLAKVPAVQTA 327 Query: 250 IDLATSSLR 258 + A L+ Sbjct: 328 MRQAGEELK 336 >gi|300311659|ref|YP_003775751.1| esterase/lipase [Herbaspirillum seropedicae SmR1] Length = 317 Score = 135 bits (340), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 76/214 (35%), Positives = 114/214 (53%), Gaps = 5/214 (2%) Query: 26 VVMYFHGAGWVMGDVNTHDRLVRQIVDGAKVAVVFVDYDRSPEARYPIAIEQDYAATKYI 85 V++YFHG G+ +G +++HD L + V+ VDY +PE R+P A E A ++ Sbjct: 86 VLLYFHGGGYCVGSLDSHDTLCSALAARTPCCVLAVDYRLAPEHRFPTAFEDAADAYDWL 145 Query: 86 AEHAAEFNVDASRLAIAGDSVGGN-MTAVVSLLAKERKGPAIKYQVLFYPVTDTNFENGS 144 +H + +RLA+ GDS GG A+ + + P + QVL YP T + + S Sbjct: 146 LQHGPALGLATNRLAVGGDSAGGTLAAALALRARQAARAPCL--QVLLYPCTSAHQDGDS 203 Query: 145 YKQFANGPWLTKEAMKWFWNAYLPAGANRKDPHIAPLNATLEQLKDLPPALVITDENDVL 204 +++FA G L E ++W + L R+D APL A + PALV+ ++D L Sbjct: 204 HRRFATGYLLEGETLQWMFGHTLNGDHERQDWRFAPLQAA--DVSGAAPALVVIGDHDPL 261 Query: 205 RDEGEAYAAKLAKAGVPVTQVRYLGTIHDFVMLN 238 DEG AYA KL +AGVPV + G +HDF+ L Sbjct: 262 LDEGMAYAEKLRQAGVPVQLDIHPGMVHDFLRLG 295 >gi|300309659|ref|YP_003773751.1| esterase-lipase superfamily protein [Herbaspirillum seropedicae SmR1] Length = 332 Score = 122 bits (307), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 83/234 (35%), Positives = 120/234 (51%), Gaps = 12/234 (5%) Query: 5 GPTGSTRIRILRP-----QGNKETLPVVMYFHGAGWVMGDVNTHDRLVRQIVDGAKVAVV 59 GP G +R+ P +G K P+VMYFHG G+V G + D R I + ++AV+ Sbjct: 61 GPLGRIPVRLYLPATPLARGQKR--PLVMYFHGGGFVSGSLEDADTPARFIAEHGQLAVL 118 Query: 60 FVDYDRSPEARYPIAIEQDYAATKYIAEHAAEFNVDASRLAIAGDSVGGNMTAVVSLLAK 119 V Y +P +P A E +AA + ++A E VD R+ +AGD GG + A ++L+ + Sbjct: 119 SVGYALAPAKPFPAAPEDAFAAATWACKNATELKVDLDRMVVAGDDAGGCLAASLTLMTR 178 Query: 120 ERKGPAIKYQVLFYPVTDTNFE-NGSYKQFANGPWLTKEAMKWFWNAYLPAGANRKDPHI 178 +R G + QVL P+ D + G + + LT E + YLP R P+ Sbjct: 179 DRCGKPLAAQVLISPMLDPSMRLLGDAARLQSD--LTAETCAASYRQYLPQTMQRLHPYA 236 Query: 179 APLNATLEQLKDLPPALVITDENDVLRDEGEAYAAKLAKAGVPVTQVRYLGTIH 232 APL + +L LPPA + T E DVL E E YA+ L +AGVP R+ G H Sbjct: 237 APLEVS--RLAGLPPAFIATAERDVLCPEAEKYASSLIRAGVPTQVSRFAGITH 288 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 865,522 Number of extensions: 36126 Number of successful extensions: 107 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 104 Number of HSP's successfully gapped: 3 Length of query: 263 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 180 Effective length of database: 1,226,865 Effective search space: 220835700 Effective search space used: 220835700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)