BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0216.0009.p
         (398 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310451|ref|YP_003774543.1| 2-keto-4-pentenoate hydratase/2...   608   e-175

>gi|300310451|ref|YP_003774543.1| 2-keto-4-pentenoate
           hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase
           [Herbaspirillum seropedicae SmR1]
          Length = 448

 Score =  608 bits (1567), Expect = e-175,   Method: Compositional matrix adjust.
 Identities = 291/393 (74%), Positives = 326/393 (82%), Gaps = 2/393 (0%)

Query: 2   SIILNETHDADLRSWVASANAAGSDFPIQNLPYGVFRRSGREGDEVFRAGVAIGDSILDL 61
           S+ LNETHD +LRSWV SANA G  FPIQNLPYGVFRR+G E D  FR GVAIGD ILDL
Sbjct: 5   SLTLNETHDTELRSWVPSANADGCPFPIQNLPYGVFRRAGSEED--FRVGVAIGDQILDL 62

Query: 62  RAAFESGAFAGFGETVHKAIVAATAGSNLNPLMELDAAAWSALRLALSTLLRDGSVHQSQ 121
            AA  +GAF+GF E   +A  AA +GS LN LM L  A+WSALRLALS LLR  S HQ++
Sbjct: 63  PAAQSAGAFSGFDEATRQAAHAACSGSRLNALMALTPASWSALRLALSHLLRTASPHQTE 122

Query: 122 LQAALVPQAEAEHALPAQIGDYTDFYTSIHHATAIGKLFRPDNPLLPNYKWVPIGYHGRS 181
           L A LVPQA+AE+ +PA IGDYTDFYTSIHHAT +GKLFRPDNPLLPNYKWVPIGYHGR 
Sbjct: 123 LAACLVPQAQAEYDVPAAIGDYTDFYTSIHHATTVGKLFRPDNPLLPNYKWVPIGYHGRV 182

Query: 182 SSIAVSGQQFRRPLGQLKSPTSDTPVFGACKRLDYEMEVGVFMGQGNAIGDAIAIGAAES 241
           SSIAVSGQQFRRPLGQ K+P +D P FG CKRLDYE+E+G+F+G GNA+G+ + +  AE 
Sbjct: 183 SSIAVSGQQFRRPLGQTKAPDADAPTFGPCKRLDYELEIGIFIGGGNAMGEPVPMEEAED 242

Query: 242 HVFGLCLLNDWSARDVQAWEYQPLGPFLAKNFASTISPWIVTIEALAPYRAAWTRDAADP 301
            VFGLCLLNDWSARDVQAWEYQPLGPFL+K+FASTISPWIVT+EALAPYR +W+R   DP
Sbjct: 243 KVFGLCLLNDWSARDVQAWEYQPLGPFLSKSFASTISPWIVTLEALAPYRCSWSRPDGDP 302

Query: 302 QPLAYLDAPEVREHGAFDVQLEVLLQTKAMREAGTAPQRLSLSNFRDAYWTVSQLVAHHT 361
           QPL YLDAP +R  GAFDVQLEVL+QT AMR+AG  PQRLSLSN+RDAYWTV+QLV HHT
Sbjct: 303 QPLPYLDAPTLRTQGAFDVQLEVLIQTAAMRQAGQPPQRLSLSNYRDAYWTVAQLVTHHT 362

Query: 362 VGGCNLRAGDLLGSGTLSGPTPESAGSLLELTN 394
           V GCNLR GD LGSGTLSGP P+ AGSLLELT 
Sbjct: 363 VNGCNLRPGDFLGSGTLSGPAPDEAGSLLELTT 395


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.318    0.133    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,204,908
Number of extensions: 47715
Number of successful extensions: 125
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 125
Number of HSP's successfully gapped: 1
Length of query: 398
Length of database: 1,619,870
Length adjustment: 87
Effective length of query: 311
Effective length of database: 1,207,925
Effective search space: 375664675
Effective search space used: 375664675
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 131 (55.1 bits)