BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0216.0007.p (400 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310449|ref|YP_003774541.1| 4-hydroxybenzoate transporter t... 608 e-175 gi|300310645|ref|YP_003774737.1| major facilitator superfamily b... 224 8e-60 gi|300310481|ref|YP_003774573.1| major facilitator superfamily p... 188 7e-49 gi|300310615|ref|YP_003774707.1| major facilitator superfamily p... 107 1e-24 gi|300310666|ref|YP_003774758.1| major facilitator superfamily p... 88 1e-18 >gi|300310449|ref|YP_003774541.1| 4-hydroxybenzoate transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 447 Score = 608 bits (1568), Expect = e-175, Method: Compositional matrix adjust. Identities = 313/388 (80%), Positives = 349/388 (89%) Query: 6 INVDQVVEQSRFGAFQFGLLLLCGLCLIIDGFDIQAMGYVAPAIIGDWHVNKASLGPVFG 65 ++VDQVVEQ RFGAFQFGLLLLCGLCLIIDGFD+QAMGYVAPAII DW V+KASLGPVFG Sbjct: 12 VHVDQVVEQGRFGAFQFGLLLLCGLCLIIDGFDVQAMGYVAPAIIADWGVSKASLGPVFG 71 Query: 66 AGLFGMLLGSLVLTPVGDKYGRRPVLIISTFFFAACMLLTTRVQTIDELLVLRFITGFGL 125 AGLFGMLLGSLVLTPVGD+YGRRPVLI+ST FFA CML T V T+D+LLVLRFITGFGL Sbjct: 72 AGLFGMLLGSLVLTPVGDRYGRRPVLILSTLFFALCMLATPMVTTLDQLLVLRFITGFGL 131 Query: 126 GSIMPNAMALVGEFSPNSSRVTRMMLVSCGFTVGAAVGGFVSAALIPAFGWRSVFLVGGL 185 GSIMPNAMALVGEFSP+SSRVTRMMLVSCGFTVGAA GGFVSAALIPA+GW +VF VGG Sbjct: 132 GSIMPNAMALVGEFSPSSSRVTRMMLVSCGFTVGAAAGGFVSAALIPAYGWHAVFWVGGA 191 Query: 186 IPAALGVAMLIWLPESIQFLVLRKRPMKQVARWLNKVQPGLLVTESSTVVVKEARNDGMP 245 +P LG+AML+WLPESIQFLVL +RP QVARWL K+ P +++ + + VVVKE + +GMP Sbjct: 192 VPLVLGLAMLVWLPESIQFLVLHRRPRTQVARWLRKLDPNIVIDDKTEVVVKETKAEGMP 251 Query: 246 VAELFREGRAGVTLLLWLISFMNLINLYFLSNWLPTLIKDAGYSTGAAVLIGTSLQVGGI 305 VA LFR+GRAGVT+LLWLISFMNLINLYFLSNWLPTLI+DAGYST AVLIGTSLQVGG+ Sbjct: 252 VAALFRDGRAGVTVLLWLISFMNLINLYFLSNWLPTLIRDAGYSTSMAVLIGTSLQVGGV 311 Query: 306 VGTLTLGWFINRFGFTRVLGSCFVVACVFIALIGHVAATPVLLFICVVVAGFCVVGGQPA 365 +GTL+LG FI RFGFTRVLGSCF++AC+ IALIG +A P LLF+ V+VAGFC+VGGQPA Sbjct: 312 IGTLSLGRFIQRFGFTRVLGSCFLLACLSIALIGKLATVPALLFVAVIVAGFCIVGGQPA 371 Query: 366 VNALAGTFYPTQLRSTGIGWALGIGRGG 393 VNALAGTFYPT LRSTGIGWALGIGR G Sbjct: 372 VNALAGTFYPTTLRSTGIGWALGIGRIG 399 >gi|300310645|ref|YP_003774737.1| major facilitator superfamily benzoate permease [Herbaspirillum seropedicae SmR1] Length = 455 Score = 224 bits (572), Expect = 8e-60, Method: Compositional matrix adjust. Identities = 135/390 (34%), Positives = 223/390 (57%), Gaps = 3/390 (0%) Query: 4 DVINVDQVVEQSRFGAFQFGLLLLCGLCLIIDGFDIQAMGYVAPAIIGDWHVNKASLGPV 63 ++V Q+++ +RF F + ++ LC L L+ DG+D+ G V P ++ +W ++ G + Sbjct: 2 KTLDVSQIIDGARFTKFHWMVMCLCALLLVFDGYDLFIYGAVLPVLMKEWSLSPVQAGAL 61 Query: 64 FGAGLFGMLLGSLVLTPVGDKYGRRPVLIISTFFFAACMLLTTRVQTIDELLVLRFITGF 123 LFGM+ G+ + P+ D+ GR+ + IS F+ L+ + E V RF+ G Sbjct: 62 GSYALFGMMFGAFIFGPLADRIGRKKGVAISFVLFSVSTFLSGFATSPTEFGVYRFLAGL 121 Query: 124 GLGSIMPNAMALVGEFSPNSSRVTRMMLVSCGFTVGAAVGGFVSAALIPAFGWRSVFLVG 183 G G +MPNA+AL+ E++P R T + ++ G+++G + + ++P FGW+S+F Sbjct: 122 GCGGLMPNAVALMNEYAPKRLRSTLVAIMFSGYSLGGVLCAGLGIWMLPNFGWQSMFFAA 181 Query: 184 GLIPAALGVAMLIWLPESIQFLVLRKRPMKQVARWLNKVQPGLLVTESSTVVVKEARNDG 243 +P L +L LPES+ FL LR+ ++Q L +V P + + +V + + G Sbjct: 182 A-VPLVLLPVILWKLPESVGFL-LRQGRLEQARAMLTQVDPQARIATDTQLVQADVKGAG 239 Query: 244 MPVAELFREGRAGVTLLLWLISFMNLINLYFLSNWLPTLIKDAGYSTGAAVLIGTSLQVG 303 P+ LF++ RA TL++W+ F L+ +Y L +WLP L+ AGYS G+++ +L G Sbjct: 240 APMLHLFKQDRAVGTLMIWVSFFCCLLMVYALGSWLPKLMASAGYSLGSSLSFLLALNFG 299 Query: 304 GIVGTLTLGWFINRFGFTRVLGSCFVVACVFIALIGHVAATPVLLFICVVVAGFCVVGGQ 363 G+VG + GW +RF +V+ S F V V IAL+G + TPV L++ ++VAG +G Q Sbjct: 300 GVVGAIGGGWLGDRFTLPKVVVSFFTVGTVAIALLGFNSPTPV-LYLLIIVAGATTIGTQ 358 Query: 364 PAVNALAGTFYPTQLRSTGIGWALGIGRGG 393 + A FYP +RSTG+GWA G+GR G Sbjct: 359 ILLYANTAQFYPLAIRSTGLGWASGVGRSG 388 >gi|300310481|ref|YP_003774573.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 460 Score = 188 bits (477), Expect = 7e-49, Method: Compositional matrix adjust. Identities = 118/394 (29%), Positives = 194/394 (49%), Gaps = 4/394 (1%) Query: 3 SDVINVDQVVEQSRFGAFQFGLLLLCGLCLIIDGFDIQAMGYVAPAIIGDWHVNKASLGP 62 + +++ ++++ F FQ ++ L L +++DGFD Q +G+ P++I +W + + + P Sbjct: 9 NQTVDIGRMLDDGVFSGFQKLVVCLAALSIVMDGFDGQLIGFAIPSLIKEWGITRNAFAP 68 Query: 63 VFGAGLFGMLLGSLVLTPVGDKYGRRPVLIISTFFFAACMLLTTRVQTIDELLVLRFITG 122 AGL GM +GS D++GRR +I S F F L + + +LRF G Sbjct: 69 AVAAGLVGMGIGSAFAGLFADRFGRRMAIIGSVFVFGVATSLIAFSPDVTTIAILRFCAG 128 Query: 123 FGLGSIMPNAMALVGEFSPNSSRVTRMMLVSCGFTVGAAVGGFVSAALIPAFGWRSVFLV 182 G+G +P++ + EFSP R + +G + G + A++P +GWR +FL+ Sbjct: 129 LGIGGALPSSTTMTAEFSPARRRTLAVTATIVCVPLGGMLAGLFANAVLPHYGWRGLFLI 188 Query: 183 GGLIPAALGVAMLIWLPESIQFLVLRKRPMKQVARWLNKVQPGLLVTESSTVVVKEARND 242 GGL+P A V + +PES ++L ++ R L ++ + ++A Sbjct: 189 GGLLPVAFSVLLWFVIPESPRYLARHPHRWDELRRLLQRMSRPVSADTGFADAREQAIET 248 Query: 243 GMPVAELFREGRAGVTLLLWLISFMNLINLYFLSNWLPTLIKDAGYSTGAAVLIGTSLQV 302 LF G+A T+ LW+ FMNL+ +Y +WLPT++ G S A T+ Sbjct: 249 RAGFGALFASGQARDTMALWVAFFMNLLAVYSAFSWLPTMLAGEGLSPSVAGAGLTAYNF 308 Query: 303 GGIVGTLTLGWFINRFGFTRVLGSCFVVA---CVFIALIGHVAATPVLLFICVVVAGFCV 359 GG+ G L FI RFG + C + A +F+ + VA+ LL + + + G V Sbjct: 309 GGVFGALLCALFITRFGSRLPMLLCCLAATASALFLRSL-DVASQTTLLVVGIGLHGLFV 367 Query: 360 VGGQPAVNALAGTFYPTQLRSTGIGWALGIGRGG 393 Q + AL YPTQ+R+TG AL GR G Sbjct: 368 NAVQSTMYALCAYIYPTQVRATGTAAALAFGRLG 401 >gi|300310615|ref|YP_003774707.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 478 Score = 107 bits (268), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 96/394 (24%), Positives = 175/394 (44%), Gaps = 25/394 (6%) Query: 12 VEQSRFGAFQFGLLLLCGLCLIIDGFDIQAMGYVAPAIIGDWHVNKASLGPVFGAGLFGM 71 +E F + ++ G + D F ++ +V P ++G W ++ A +G + +G G Sbjct: 33 IENVPFSRWHTKARIIMGSATLFDAFHALSLAFVLPVLVGLWQLSAAQIGLLIASGYLGQ 92 Query: 72 LLGSLVLTPVGDKYGRRPVLIISTFFFAACMLLTTRVQTIDELLVLRFITGFGLGSIMPN 131 ++G+L + ++YGR P ++ + ++ + L + RF+ G G+G +P Sbjct: 93 VIGALFFGWMAERYGRVPSASVTVGLMSVLSIVCALTGSFHMLFLCRFLQGIGVGGEVPV 152 Query: 132 AMALVGEFSPNSSRVTRMMLVSCGFTVGAAVGGFVSAALIPAFGWRSVFLVGGLIPAALG 191 A + E S R +L F +G V A L+P FGW +FLVGG IP L Sbjct: 153 AATYINELSQAHGRGKFFVLYELIFPLGLLAAAQVGAFLVPRFGWEYMFLVGG-IPGILV 211 Query: 192 VAMLIWLPESIQFLVLRKR--PMKQVARWLNKVQPGLLVTESSTVVVKEARNDGMPV--- 246 ++ LPES ++L+ R R +++ R L P + + E R + + Sbjct: 212 ALLITRLPESPRWLIARGRLDDAEKLIRTLEASTPKRNLDAQTDKQAIEQRINALAAGAG 271 Query: 247 -------AELFREGRAGVTLLLWLISFMNLINLYFLSNWLPTLIKDAGYSTGAAVLIGTS 299 ELF G T+++WL+ + ++NWLP+L Y T + + S Sbjct: 272 KRQRGSWKELFSPVYRGRTIIVWLLWASSYFVANGINNWLPSL-----YKTVYHLPLQES 326 Query: 300 LQVGGIVGTLT------LGWFINRFGFTRVLGSCFVVACVFIALIGHVAATPVLLFICVV 353 L++ + L+ I+R G R + F+++ +AL+G + A + + Sbjct: 327 LRMASVSNVLSACAVLGCALLIDRVGRRRWAIASFILSGALLALLGVLKAESAWSVMFLA 386 Query: 354 VAGFCVVGGQPAVNAL-AGTFYPTQLRSTGIGWA 386 + + V+G + L YPT++R+ G G A Sbjct: 387 SSAYAVMGTTTVLLYLYTPEIYPTRMRAIGTGLA 420 >gi|300310666|ref|YP_003774758.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 438 Score = 88.2 bits (217), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 105/406 (25%), Positives = 186/406 (45%), Gaps = 23/406 (5%) Query: 1 MQSDVINVDQVVEQSRFGAFQFGLLLLCGLCLIIDGFDIQAMGYVAPAIIGDWHV---NK 57 M + +V+Q ++ + G FQ+ L ++ GL + D + ++G+ AP I + + Sbjct: 1 MSTLPASVEQGLQAAGVGKFQYRLFVIFGLVWLADAMQVLSIGFSAPTIAKTFGLTVPQA 60 Query: 58 ASLGPVFGAGLFGMLLGSLVLTPVGDKYGRRPVLIISTFFFAACMLLTTRVQTIDELLVL 117 G +F GMLLG+ V + D+ GRRPVL+ + A + + LL L Sbjct: 61 LQTGTMF---FVGMLLGAFVFGRLADRIGRRPVLMGAVVIDACFGVASAFAPDFTWLLAL 117 Query: 118 RFITGFGLGSIMPNAMALVGEFSPNSSRVTRMMLVSCGFTVGA---AVGGFVSAALIPAF 174 R +TG G+G +P ++ EF P++ R ++++ + +G AV V+ + Sbjct: 118 RLLTGIGVGGTLPVDYTMMAEFLPSARRGRWLVMLESFWAIGTICLAVLALVAVSW-GND 176 Query: 175 GWRSVFLVGGLIPAALGVAMLIWLPESIQFLVL--RKRPMKQVARWLNKVQPGLLVTESS 232 WR +F V GL PA +GV + ++PES +L + +QV + KV SS Sbjct: 177 AWRVIFFVTGL-PALVGVVLRFYVPESPMYLNRSGKSDAARQVLERVAKVN------GSS 229 Query: 233 TVV--VKEARNDGMPVAELFREGRAGVTLLLWLISFMNLINLYFLSNWLPTLIKDAGYST 290 T + ++ + P++ LF +L L+L + I Y + +LP + G+ Sbjct: 230 TPIPPLQPEVVERKPMSALFSAALRRRSLSLYLAWGLISIAYYGVFVYLPVKLGSEGFGF 289 Query: 291 GAAVLIGTSLQVGGIVGTLTLGWFINRFGFTRVLGSCFVVACVFIALIGHVAATPVLLFI 350 + L + + G + + R+G L V++ V L + P+L+ Sbjct: 290 MRGQIFLIFLALVQLPGYALSAYGVERWGRKPTLVGFLVLSAVGCMLY-SLGKDPMLVVG 348 Query: 351 CVVVAGFCVVGGQPAVNALAGTFYPTQLRSTGIGWALGIGR-GGFF 395 ++ F ++G A+ A YPT LR++G+G A + R GG F Sbjct: 349 STLLLSFSLLGTWAALYAFTPEVYPTDLRASGMGTAGAVARFGGLF 394 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.144 0.450 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,109,320 Number of extensions: 43890 Number of successful extensions: 366 Number of sequences better than 1.0e-08: 22 Number of HSP's gapped: 360 Number of HSP's successfully gapped: 22 Length of query: 400 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 313 Effective length of database: 1,207,925 Effective search space: 378080525 Effective search space used: 378080525 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)