BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0216.0004.p
         (182 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310447|ref|YP_003774539.1| esterase [Herbaspirillum serope...   101   3e-23
gi|300312950|ref|YP_003777042.1| lipase [Herbaspirillum seropedi...    81   4e-17
gi|300311008|ref|YP_003775100.1| esterase/lipase/thioesterase fa...    56   2e-09

>gi|300310447|ref|YP_003774539.1| esterase [Herbaspirillum
           seropedicae SmR1]
          Length = 277

 Score =  101 bits (252), Expect = 3e-23,   Method: Compositional matrix adjust.
 Identities = 60/118 (50%), Positives = 77/118 (65%), Gaps = 9/118 (7%)

Query: 1   VAMINYRLAPEVDMPEIVGDVRLAFSWLYRQAAQLGFNADDIYVAGHSAGGHLAAILAAT 60
           VA+INYRLAP+V M +I  D   A   L+ +A  L  +A+ I++ GHSAG HLA ++AA 
Sbjct: 102 VALINYRLAPQVRMGDIAADCGRALRLLHAKAPALRVDANAIWLMGHSAGAHLACVIAA- 160

Query: 61  DWQDSGLPDNAVKGLCSVSGLYDLEPIRLCYLNEALQLDQSQVQQFSPLHHLPRLPXG 118
                 L    V+G+C++SGLYDLEPIRL YLNEAL L  + VQQ SPL  L  +P G
Sbjct: 161 ------LRAAPVRGVCALSGLYDLEPIRLSYLNEALHLSPADVQQASPL--LLAMPDG 210


>gi|300312950|ref|YP_003777042.1| lipase [Herbaspirillum seropedicae
           SmR1]
          Length = 289

 Score = 81.3 bits (199), Expect = 4e-17,   Method: Compositional matrix adjust.
 Identities = 46/109 (42%), Positives = 70/109 (64%), Gaps = 6/109 (5%)

Query: 1   VAMINYRLAPEVDMPEIVGDVRLAFSWLYRQAAQLGFNADDIYVAGHSAGGHLAAILAAT 60
           VA+INY LAP+  + ++V +V+   S + +QA +LG + D I + GHSAGGHLAA +AA 
Sbjct: 112 VAVINYSLAPQARIEDMVKEVQACLSTIAQQAERLGIDVDRISLMGHSAGGHLAAFVAA- 170

Query: 61  DWQDSGLPDNAVKGLCSVSGLYDLEPIRLCYLNEALQLDQSQVQQFSPL 109
             Q   +P   V+ + ++SG++DL P+    LN+AL LDQ +    SP+
Sbjct: 171 --QPGRMP---VQAVFAISGVFDLAPLIPTSLNKALTLDQVRADALSPV 214


>gi|300311008|ref|YP_003775100.1| esterase/lipase/thioesterase
           family protein [Herbaspirillum seropedicae SmR1]
          Length = 302

 Score = 56.2 bits (134), Expect = 2e-09,   Method: Compositional matrix adjust.
 Identities = 44/148 (29%), Positives = 65/148 (43%), Gaps = 19/148 (12%)

Query: 5   NYRLAPEVDMPEIVGDVRLAFSWLYRQAAQLGFNADDIYVAGHSAGGHLAAILAA-TDWQ 63
           +YRL PEV  P+ + D   A +W  R    LG +   ++V GHSAG + AA++A    W 
Sbjct: 110 DYRLYPEVSYPDFLQDSAQAVAWTLRSLQALGGDPQRVFVMGHSAGAYNAAMMALDARWL 169

Query: 64  D-SGLPDNAVKGLCSVSGLYDLEPIRLCYLNEALQLDQSQVQQFSPLHHLPRLPXGDPPP 122
           D +G     ++G   ++G YD  PI              +     P+ H P  P    P 
Sbjct: 170 DAAGATPAQLRGWIGLAGPYDFLPI--------------ENPDVKPVFHFPDSPPDSQPV 215

Query: 123 R---ARSPWAISTKPERSIACSASRTAG 147
           R   A +P A+     +    SA+R  G
Sbjct: 216 RHVQASAPPALLIASHQDKLVSATRNTG 243


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.319    0.134    0.422 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 482,446
Number of extensions: 16754
Number of successful extensions: 63
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 61
Number of HSP's successfully gapped: 3
Length of query: 182
Length of database: 1,619,870
Length adjustment: 80
Effective length of query: 102
Effective length of database: 1,241,070
Effective search space: 126589140
Effective search space used: 126589140
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 127 (53.5 bits)