BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0215.0059.p (233 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrog... 196 2e-51 gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde deh... 172 3e-44 gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 154 5e-39 gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrog... 151 4e-38 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 150 8e-38 >gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 504 Score = 196 bits (497), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 95/228 (41%), Positives = 142/228 (62%), Gaps = 4/228 (1%) Query: 1 MVFDDADLDHAVAGVLYGIFSSSGESCIAGSRLFVARGIYDSFMERLAAGAAALRVGDPA 60 ++F+DADL A G IFS +G+SC+AGSRL V R +YD F+E +AAGA +R+G P Sbjct: 273 IIFEDADLRLAAFGAQAAIFSGAGQSCVAGSRLLVQRKVYDRFVETVAAGAEKIRLGAPL 332 Query: 61 DERTQMGPLITARHREGIESYVSIGVADGGRIRTGGTRLAGDQFARGYYYAPTIIEGVTN 120 D+ T++GP+ + E I+ V+ G+ G + G TR + GY+ PT++ +N Sbjct: 333 DDSTEVGPINNRKQYEHIQRMVARGLEAGATLAAGHTRYGEE----GYFVRPTLLAHASN 388 Query: 121 DQRICQEEIFGPVLVAMPFDDEEDLIAQANDSVYALAAGIWTRDYKKAWRFGRAVQAGNV 180 + + EIFGPV VA+PF+DEE+ IA ANDS + LA +WTRD +A R +V AG Sbjct: 389 AMEVARSEIFGPVAVAIPFEDEEEAIAIANDSEFGLAGAVWTRDVARAHRVAASVNAGTF 448 Query: 181 WINTYKQFSISTPFGGWRDSGLGREKGRLGILQYMEQKSMYWGLNENP 228 W+N+YK ++++PFGG+ SG GR G + Y + KS++ + +P Sbjct: 449 WVNSYKTINVASPFGGFNRSGYGRSSGMEALYDYTQTKSVWVETSASP 496 >gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 172 bits (435), Expect = 3e-44, Method: Compositional matrix adjust. Identities = 87/207 (42%), Positives = 125/207 (60%), Gaps = 5/207 (2%) Query: 17 YGIFS-SSGESCIAGSRLFVARGIYDSFMERLAAGAAALRVGDPADERTQMGPLITARHR 75 + +F+ + GE C SR+ V IY+ F+ER AA++ G+P D+ T +G + Sbjct: 290 FAMFALNQGEVCTCPSRVLVQESIYERFIERALKRVAAIKQGNPLDKSTMIGAQASQEQL 349 Query: 76 EGIESYVSIGVADGGRIRTGGTR--LAGDQFARGYYYAPTIIEGVTNDQRICQEEIFGPV 133 E I SY+ IG +G ++ GG R L GD A GYY PT+ +G N RI QEEIFGPV Sbjct: 350 EKILSYIDIGKQEGAKVLAGGGREELGGD-LASGYYVKPTVFQG-NNKMRIFQEEIFGPV 407 Query: 134 LVAMPFDDEEDLIAQANDSVYALAAGIWTRDYKKAWRFGRAVQAGNVWINTYKQFSISTP 193 + F DEE+ +A AND++Y L AG+WTRD +A+R GR +QAG VW N Y + Sbjct: 408 VSVTTFKDEEEALAIANDTLYGLGAGLWTRDGTRAFRMGREIQAGRVWTNCYHLYPAHAA 467 Query: 194 FGGWRDSGLGREKGRLGILQYMEQKSM 220 FGG++ SG+GRE ++ + Y + K++ Sbjct: 468 FGGYKQSGIGRENHKMMLDHYQQTKNL 494 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 154 bits (390), Expect = 5e-39, Method: Compositional matrix adjust. Identities = 85/220 (38%), Positives = 123/220 (55%), Gaps = 4/220 (1%) Query: 1 MVFDDADLDHAVAGVLYGIFSSSGESCIAGSRLFVARGIYDSFMERLAAGAAALRVGDPA 60 +VFDDA + A+ G G+F + G+ C AGSR+ V R + F + LA AA + VGDP+ Sbjct: 263 IVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQRSLAQRFAKALAEAAAGITVGDPS 322 Query: 61 DERTQMGPLITARHREGIESYVSIGVADGGRIRTGGTRLAGDQFARGYYYAPTIIEGVTN 120 +MGP+ E + Y+ G+ +G + GG + +G++ PTI N Sbjct: 323 QPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGGV----SRPDKGWFVQPTIFADANN 378 Query: 121 DQRICQEEIFGPVLVAMPFDDEEDLIAQANDSVYALAAGIWTRDYKKAWRFGRAVQAGNV 180 I +EEIFGPV + FD EE+ IA ANDS Y LAA +WT + +A R V+ G V Sbjct: 379 GMSIAREEIFGPVGTIISFDTEEEAIALANDSKYGLAATVWTTNLARAHRVAAGVKVGAV 438 Query: 181 WINTYKQFSISTPFGGWRDSGLGREKGRLGILQYMEQKSM 220 +N + + P+GG +DSG+GRE G G L Y E+K++ Sbjct: 439 GVNCWSPLDANLPWGGIKDSGMGREGGLAGALAYTEEKTV 478 >gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 151 bits (382), Expect = 4e-38, Method: Compositional matrix adjust. Identities = 84/208 (40%), Positives = 122/208 (58%), Gaps = 3/208 (1%) Query: 1 MVFDDADLDHAVAGVLYGIFSSSGESCIAGSRLFVARGIYDSFMERLAAGAAALRVGDPA 60 +VF+DAD++ + V+ I ++G++C AGSRL V RG Y+ +ERL+ + L+VG + Sbjct: 254 IVFEDADMEAMLPVVVNAIVQNAGQTCSAGSRLLVQRGAYEQVLERLSGLFSQLQVGS-S 312 Query: 61 DERTQMGPLITARHREGIESYVSIGVADGGRIRTGGTRLAGDQFARGYYYAPTIIEGVTN 120 GPLI +E + ++S DG + G ++A G+Y AP ++ V Sbjct: 313 QMDLDCGPLIRKTQQERVAGFLSDAQRDGIALAAQG-KIAAGVPTGGFYQAPALLRDVPP 371 Query: 121 DQRICQEEIFGPVLVAMPFDDEEDLIAQANDSVYALAAGIWTRDYKKAWRFGRAVQAGNV 180 R+ QEE+FGPVL AMPF+DE IA AN + Y L A +WTRD + R RA+++G V Sbjct: 372 RHRLAQEEVFGPVLAAMPFEDEAHAIALANGTDYGLVASVWTRDGGRQMRLARAIRSGQV 431 Query: 181 WINTYKQ-FSISTPFGGWRDSGLGREKG 207 +IN Y + PFGG + SG GREKG Sbjct: 432 FINNYGAGGGVELPFGGVKASGHGREKG 459 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 150 bits (379), Expect = 8e-38, Method: Compositional matrix adjust. Identities = 79/224 (35%), Positives = 127/224 (56%), Gaps = 6/224 (2%) Query: 1 MVFDDADLDHAVAGVLYGIFSSSGESCIAGSRLFVARGIYDSFMERLAAGAAALRVGDPA 60 +VFDDADLD AV G + + ++G++C+ +R++V G+YD+F +L +VG Sbjct: 264 IVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAKLVEAVKKFKVGQGT 323 Query: 61 DERTQMGPLITARHREGIESYVSIGVADGGRIRTGGTRLAGDQFARGY-YYAPTIIEGVT 119 +E GPLI + + +E +V+ VA G R+ GG R A G+ ++ PT++ VT Sbjct: 324 EEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGKR-----HALGHSFFEPTVLADVT 378 Query: 120 NDQRICQEEIFGPVLVAMPFDDEEDLIAQANDSVYALAAGIWTRDYKKAWRFGRAVQAGN 179 ++ +EE FGP+ F +E+++A AND+ + LA+ ++RD + WR +++G Sbjct: 379 PAMQVAREETFGPMAPLFRFKTDEEVLALANDTEFGLASYFYSRDIGRIWRVAEGLESGM 438 Query: 180 VWINTYKQFSISTPFGGWRDSGLGREKGRLGILQYMEQKSMYWG 223 V INT + PFGG + SGLGRE GI Y+ K + G Sbjct: 439 VGINTGLISNEVAPFGGVKQSGLGREGSHYGIDDYLVVKYLCMG 482 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.139 0.431 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 806,832 Number of extensions: 34186 Number of successful extensions: 130 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 109 Number of HSP's successfully gapped: 19 Length of query: 233 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 151 Effective length of database: 1,231,600 Effective search space: 185971600 Effective search space used: 185971600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)