BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0215.0048.p (344 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313395|ref|YP_003777487.1| porin transmembrane protein [He... 168 6e-43 gi|300310328|ref|YP_003774420.1| porin signal peptide protein [H... 144 7e-36 gi|300313813|ref|YP_003777905.1| transmembrane outer membrane po... 142 4e-35 gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) ... 140 2e-34 gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) ... 130 1e-31 >gi|300313395|ref|YP_003777487.1| porin transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 168 bits (426), Expect = 6e-43, Method: Compositional matrix adjust. Identities = 125/330 (37%), Positives = 174/330 (52%), Gaps = 23/330 (6%) Query: 18 FAQSNVTIYGSLDAGVAYISNLGGGSVARLDQGTMQPDRIGFRGTEDLGGNLKANFQLES 77 +AQS+VT+YG++D G+AY SN GG S ++ G + ++G GTEDLGG A F+LE+ Sbjct: 32 WAQSSVTLYGTVDLGLAYSSNQGGASNTYMNSGALAASKLGLTGTEDLGGGNMALFRLEN 91 Query: 78 GFYTDSGAQPTAGKLFNRYSTIGLSG-DFGAVTMGHMPDIVFDYVGKLSNGYQLTNWYLF 136 GF D+GAQ TAG LFNR S +GLS D+G VT+G F YVG L LT Sbjct: 92 GFNADTGAQSTAGVLFNRQSYVGLSSKDYGQVTVGRQYTPYFQYVGALGPTNVLTGATGA 151 Query: 137 HPGNLDSLANTYQFDNAVRYTSPTFSGLQLSGMYGFGEVAGDSNNGRNVSTGATYTNGPL 196 HPG++D+L T + +N++ YT PT GLQ YG GE AG + NG +VS Y Sbjct: 152 HPGDIDALDTTLRMNNSITYTLPTLGGLQAGVQYGMGEQAGSAANGSSVSAALRYDYQAF 211 Query: 197 RAALAYSKLNNRAAG-----YG--GAFLQS--LGLGTAATVFSSLSTTAAGVGYTIGPVR 247 + Y++L N A G +G G+F + + G A+ + L TAA YT G + Sbjct: 212 SWSAGYTRLKNLANGNTAGSFGNVGSFANNSPVNAGYASADSTQLLATAA--RYTRGDLM 269 Query: 248 LNADYTQVKISLPTGVTPKQ----KNFDLGAAWRYATADTLNFGYGLSKLES-------A 296 L +Y+ V+ Q L ++ + A L GY + ++ A Sbjct: 270 LGLNYSNVQYKAGALSAFSQTAVFNTIGLIGSYNVSPALVLAAGYSYTAEKARNGISSPA 329 Query: 297 RWNTFSLSHVHAFSKRTEFYVQAAYQRAGG 326 ++N SL V++ SKRT Y AYQRA G Sbjct: 330 KYNQLSLEEVYSLSKRTALYAIQAYQRASG 359 >gi|300310328|ref|YP_003774420.1| porin signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 405 Score = 144 bits (364), Expect = 7e-36, Method: Compositional matrix adjust. Identities = 101/299 (33%), Positives = 155/299 (51%), Gaps = 28/299 (9%) Query: 1 MKKVLFAVAATG-FAASAFAQSN-VTIYGSLDAGVAYISNLGG-----GSVARLDQGTMQ 53 MKK L A+A G FA +A AQS+ +TIYG++DA + Y + + GS+ ++ G ++ Sbjct: 1 MKKSLLALAVLGAFAGAAHAQSSSITIYGAIDASLTYTNKIATDGKNTGSLTAVESGLVK 60 Query: 54 PDRIGFRGTEDLGGNLKANFQLESGFYTDSGAQPTAGKLFNRYSTIGLSGDFGAVTMGHM 113 R+G +G+EDLGG LKA F LE+GF D+G ++ R +T+GLSG+FG V +G Sbjct: 61 GSRLGIKGSEDLGGGLKAVFTLENGFNVDTGEAGQGKLMWGRQTTVGLSGNFGTVLVGRQ 120 Query: 114 PDIVFDYVGKLSNGYQLTNWYLFHPGNLDSLANTYQFDNAVRYTSPTFSGLQLSGMYGFG 173 D + D +G +++ H LD + + +N++RY SP F+GL SG+YGFG Sbjct: 121 NDPLND-IGGIASAGGFGVPGTVHSKGLDR-TDGQRLNNSIRYNSPNFNGLSFSGIYGFG 178 Query: 174 EVAGDSNNGRNVSTGATYTNGPLRAALAY------------SKLNNRAAGYGGAFLQSLG 221 E G + GR++ G+ Y NGPL L Y S +N +AA G G Sbjct: 179 EQVGRAVGGRSIGLGSQYVNGPLSLGLGYFQSKMGGGSDGSSDVNAKAACNGAGEKDHAG 238 Query: 222 LGTAATVFSSLSTTAAGVGYTIGPVRLNADYTQVKISLPTGVTPKQKNFDLGAAWRYAT 280 + L T Y GP ++ ++ V++ L T +K+ + GA + T Sbjct: 239 -------DTCLKTWTLAAAYQFGPAKVYGAFSHVRLPLAKLHTKVRKDNNFGAMYDAKT 290 >gi|300313813|ref|YP_003777905.1| transmembrane outer membrane porin signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 354 Score = 142 bits (358), Expect = 4e-35, Method: Compositional matrix adjust. Identities = 113/322 (35%), Positives = 156/322 (48%), Gaps = 28/322 (8%) Query: 16 SAFAQSNVTIYGSLDAGVAYISNLG---GGSVARLDQGTMQPDRIGFRGTEDLGGNLKAN 72 S AQS+VTIYG +D GV Y++N G GSV R+ G RIGFRGTEDLGG LKA Sbjct: 18 SVQAQSSVTIYGLVDTGVEYLNNAGPNGAGSVTRMTSGATNTSRIGFRGTEDLGGGLKAL 77 Query: 73 FQLESGFYTDSGA-QPTAGKLFNRYSTIGLSGDFGAVTMGHMPDIVFDYVGKLSN-GYQL 130 FQLE+GF D+G+ A +LFNR ST+GL G FG V G +D++G GY Sbjct: 78 FQLENGFKLDNGSFDGDANQLFNRQSTVGLEGGFGRVVAGRSYSTTYDFLGPFDPMGYSA 137 Query: 131 TNWYLFHPGNLDSLANTYQ--FDNAVRYTSPTFSGLQLSGMYGFGEVAGDSNNGRNVSTG 188 + G + + N V+Y FSG+++ MYGFGEVAG++ NG G Sbjct: 138 NYSWATTAGATGNRKDGMPTGVSNLVKYQGE-FSGVKIGAMYGFGEVAGETRNGAKYGVG 196 Query: 189 ATYTNGPLRAALAYSKLNNRAAGYGGAFLQSLGLGTAATVFSSLSTTAAGVGYTIGPV-R 247 + GP Y + N+ A T + ++ GY + Sbjct: 197 LGWGTGPFSVVATYDRNNSATA--------------TNTPYDRATSAHLAAGYQPSEAWK 242 Query: 248 LNADYTQVKISLPTGVTPKQKNFDL-GAAWRYATADTLNFGYGLSKLESARWNT----FS 302 N Y K SL TG + +F GAA+++ A TL +++ + +S Sbjct: 243 FNLGYRYYKKSLATGAADLRSDFFWGGAAYKFTPAMTLIGAIYYQNIKNVANDADPIMYS 302 Query: 303 LSHVHAFSKRTEFYVQAAYQRA 324 L +A SKRT+ Y+ AY +A Sbjct: 303 LRLKYAMSKRTDLYLSTAYAKA 324 >gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 352 Score = 140 bits (353), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 118/357 (33%), Positives = 160/357 (44%), Gaps = 40/357 (11%) Query: 1 MKKVLFAVAA-TGFAASAFAQSNVTIYGSLDAGVAYISNL-----GGGSVARLDQGTMQP 54 MKK L A G A +A AQS+V+IYG +D G+ Y S+ GG + R+D G Q Sbjct: 1 MKKTLLVAAMLAGGATTAHAQSSVSIYGLIDLGLTYYSSAPKSGGGGARLLRMDSGIAQG 60 Query: 55 DRIGFRGTEDLGGNLKANFQLESGFYTDSGAQPTAGKLFNRYSTIGL-SGDFGAVTMGHM 113 RIGF+GTEDLGG + A F LE+GF D G+ G LF R S +GL S +G++++G Sbjct: 61 SRIGFKGTEDLGGGMNAFFTLETGFGADDGSLGQGGLLFGRQSFVGLGSTQWGSLSVGRQ 120 Query: 114 PDIVFDYVGKLSNGYQLT----NWYLFHPGNLDSLANTYQF-----DNAVRYTSPTFSGL 164 D + + G+ + G W L + N + + +N+++Y S F G Sbjct: 121 YDFMANIGGQYAMGALSPAGSFGWGLHADAAHGTALNNHIYAGDRTNNSIKYNSAKFGGF 180 Query: 165 QLSGMYGFGEVAGDSNNGRNVSTGATYTNGPLRAALAYSKLNNRAAGYGGAFLQSLGLGT 224 M G GE AG++N GR VS Y NGP ALAY+ + + + Sbjct: 181 SFGAMVGLGETAGNNNAGRTVSATGQYDNGPFSGALAYTDIKDSTGSF------------ 228 Query: 225 AATVFSSLSTTAAGVGYTIGPVRLNADYTQVKISLPTGVTPKQKNFDLGAAWRYATADTL 284 S G Y IG TQVK T + K ++LG + L Sbjct: 229 ------STRIYGGGARYQIGAFTPFGVVTQVK---NTNTSAKATTYELGTVYALTPLWDL 279 Query: 285 NFGYGLSKLESARWNTFSLSHV--HAFSKRTEFYVQAAYQRAGGXEA-PVCSGRISS 338 Y K N +L + SKRT+ YV A Y R G A PV G I S Sbjct: 280 AAAYQFQKRNQGVGNAQALVATVDYKISKRTDLYVGAVYDRDKGYNAYPVFGGGIQS 336 >gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 390 Score = 130 bits (328), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 122/383 (31%), Positives = 179/383 (46%), Gaps = 50/383 (13%) Query: 1 MKKVLFAVAA-TGFAASAFAQSNVTIYGSLDAGVAYISNL-----GGGSVARLDQGTMQP 54 MKK L A+A FA +A AQS+VTIYG +D GV Y S + G GS L+ G +Q Sbjct: 1 MKKSLLALAVLGAFAGAAQAQSSVTIYGIVDTGVVYQSKVATPTGGTGSKFSLNSGVIQG 60 Query: 55 DRIGFRGTEDLGGNLKANFQLESGFYTDSGA---QPTAGKLFNRYSTIGLSGDFGAVTMG 111 RIGF+GTEDLGG L A F LE+GF D+GA T+ LF R S +GL+G+FG+V +G Sbjct: 61 SRIGFKGTEDLGGGLSAVFNLEAGFSNDTGALDDSKTSNTLFRRKSVVGLAGNFGSVLLG 120 Query: 112 HMPDIVFDYVGKLSNGYQLTNWYLFHPGNLDSLANTYQFDNAVRYTSPTFSGLQLSGMYG 171 D D + ++ W NLD L T + +N++ YT+ +G + MYG Sbjct: 121 RQTDFA-DTISSYTSVQDFGGWTGAAGHNLDRLEGT-RTNNSISYTTANLNGFTGNLMYG 178 Query: 172 FGEVAGDSNNGRNVSTGATYTNGPLRAALAYSKLNNRAAGYGGAFLQSL-------GLGT 224 FGE AG ++ G+ G Y NGPL + Y + + + L + G Sbjct: 179 FGETAGQTSAGQAFGVGGKYDNGPLGLGINYYQSKAGSTPADASLLAATGTNQFTNGTNN 238 Query: 225 AATVFSSLSTTAAGVGYTIGPVRLNADYTQVKISLPT--------------------GVT 264 +T L+ A+ Y GP R+ ++++VK L T + Sbjct: 239 GSTAQKVLNVAAS---YQFGPARVYGNWSRVKQDLNTQANLTAAGVGSLATISNRTLAAS 295 Query: 265 PKQKNFDLGAAWRYATADTLNFGYGLSKLE------SARWNTFSLSHVHAFSKRTEFYVQ 318 K +++G A+ + + L ++ + A+ SL + SKRT+ Y Sbjct: 296 NKADIYEIGTAYSLSPSLKLLAAVDHTRAQFDGVSGKAKLTQVSLGTDYWLSKRTDLYAF 355 Query: 319 AAYQRAGGXEAPVCSGRISSTGT 341 AY +A P G S+GT Sbjct: 356 LAYSKATDLNNP---GVADSSGT 375 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.133 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,095,179 Number of extensions: 49056 Number of successful extensions: 204 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 164 Number of HSP's successfully gapped: 14 Length of query: 344 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 258 Effective length of database: 1,212,660 Effective search space: 312866280 Effective search space used: 312866280 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)