BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0215.0042.p (258 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314021|ref|YP_003778113.1| HD-GYP domain-containing protei... 344 5e-96 gi|300311984|ref|YP_003776076.1| HD-GYP domain-containing protei... 302 2e-83 gi|300310279|ref|YP_003774371.1| HD-GYP domain-containing protei... 248 3e-67 gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent p... 242 2e-65 gi|300313282|ref|YP_003777374.1| HD-GYP domain-containing protei... 218 3e-58 >gi|300314021|ref|YP_003778113.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 401 Score = 344 bits (882), Expect = 5e-96, Method: Compositional matrix adjust. Identities = 154/237 (64%), Positives = 197/237 (83%), Gaps = 2/237 (0%) Query: 12 YTYMHSVAVCALMIALGHQLGLSPEETRQAGIAGLLHDIGKMAVPADILNKPGKLTDDEF 71 YTY+HSVAVCA+MI+L +QLG++P++T+QAG+AGLLHD+GKMAVP DILNKP KLT++EF Sbjct: 161 YTYLHSVAVCAMMISLANQLGMTPQQTKQAGMAGLLHDVGKMAVPLDILNKPAKLTEEEF 220 Query: 72 TSVKKHAQAGHAILRGVDGIGDAALDVSLHHHEKIDGSGYPFNLKGNQISMVAKMGAVCD 131 SV+ H GH IL+ ++GIG+ ALDVSLHHHEKIDGSGYPFNLK + IS++AKMGAVCD Sbjct: 221 ASVRMHTVQGHGILQQIEGIGEVALDVSLHHHEKIDGSGYPFNLKADSISIMAKMGAVCD 280 Query: 132 VYDAITSNRPYKPGWEPGHSVKRMASNQGHFDETILEAFIKSVGIFPTGSCVVMHSGKVG 191 VYDAITSNRPYK GW+P S++ MA++ GHFD ++AF+K++GI+PTGSCV++ SG++G Sbjct: 281 VYDAITSNRPYKAGWDPARSIRHMAASAGHFDPITMQAFVKAIGIYPTGSCVMLQSGRLG 340 Query: 192 IVIDQHPGSLLTPKVKVFFSALTMAPLPIEIIDLATTRSGDKIASHADPAKLHLPNL 248 +V+DQ P LLTP+VKVF+S PL EIIDLA S DKI ++ADPAK + NL Sbjct: 341 VVVDQRPDQLLTPRVKVFYSTANRMPLESEIIDLA--HSSDKITAYADPAKWGIGNL 395 >gi|300311984|ref|YP_003776076.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 406 Score = 302 bits (773), Expect = 2e-83, Method: Compositional matrix adjust. Identities = 139/237 (58%), Positives = 179/237 (75%), Gaps = 2/237 (0%) Query: 12 YTYMHSVAVCALMIALGHQLGLSPEETRQAGIAGLLHDIGKMAVPADILNKPGKLTDDEF 71 YTYMHSVAVCA+MI+L QLGLS +E +QAG+AGLLHDIGKM VP ILNKP KLT++EF Sbjct: 164 YTYMHSVAVCAMMISLADQLGLSAQERQQAGMAGLLHDIGKMTVPPSILNKPSKLTEEEF 223 Query: 72 TSVKKHAQAGHAILRGVDGIGDAALDVSLHHHEKIDGSGYPFNLKGNQISMVAKMGAVCD 131 SV+ H AGH +L+ + GIGDA LDVSLHHHEK+DG+GYP+ L G ISM A+MGA+CD Sbjct: 224 VSVQSHPLAGHRLLQELPGIGDAPLDVSLHHHEKMDGTGYPYKLSGRDISMTARMGAICD 283 Query: 132 VYDAITSNRPYKPGWEPGHSVKRMASNQGHFDETILEAFIKSVGIFPTGSCVVMHSGKVG 191 VYDAITS+RPYK GW+P S++ MA ++GHFD +L+AF+K+VGI+P GSCV+M +G + Sbjct: 284 VYDAITSDRPYKSGWDPSRSIQHMAQSKGHFDFDVLQAFVKAVGIYPVGSCVLMQTGHLA 343 Query: 192 IVIDQHPGSLLTPKVKVFFSALTMAPLPIEIIDLATTRSGDKIASHADPAKLHLPNL 248 +V++Q P SLL P V++F+S + +IDL RS +KI DP K + L Sbjct: 344 MVLEQRPDSLLAPLVRIFYSTNKKIAVTPTLIDL--RRSANKIVGAVDPEKWGVTRL 398 >gi|300310279|ref|YP_003774371.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 408 Score = 248 bits (633), Expect = 3e-67, Method: Compositional matrix adjust. Identities = 114/227 (50%), Positives = 155/227 (68%) Query: 12 YTYMHSVAVCALMIALGHQLGLSPEETRQAGIAGLLHDIGKMAVPADILNKPGKLTDDEF 71 YTY+HS+AVC LM+AL LG+ ++AG+AG LHD+GK +P +ILNKP +L++ E+ Sbjct: 152 YTYLHSIAVCTLMVALARTLGMDDIACKEAGLAGYLHDVGKAFIPLEILNKPDRLSEQEY 211 Query: 72 TSVKKHAQAGHAILRGVDGIGDAALDVSLHHHEKIDGSGYPFNLKGNQISMVAKMGAVCD 131 +++H G+ L+G G+ D LDV LHHHEKIDGSGYP +GNQIS+ ++M AVCD Sbjct: 212 KIIQRHPALGYDYLKGFSGVPDYVLDVCLHHHEKIDGSGYPSRHQGNQISVYSRMAAVCD 271 Query: 132 VYDAITSNRPYKPGWEPGHSVKRMASNQGHFDETILEAFIKSVGIFPTGSCVVMHSGKVG 191 VYDA+TS RPYK GW+P S+ RMAS QGHFD I AF+K++GI+P GS V + SG+ Sbjct: 272 VYDAVTSERPYKSGWDPAESLSRMASWQGHFDRPIFLAFVKALGIYPIGSIVKLQSGRTA 331 Query: 192 IVIDQHPGSLLTPKVKVFFSALTMAPLPIEIIDLATTRSGDKIASHA 238 +VI Q+ L P VK F + ++ + EIIDL+ D IA + Sbjct: 332 LVIRQNHVDLTKPVVKAFVTTPSIEVVESEIIDLSAPGQTDAIAERS 378 >gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent phosphohydrolase [Herbaspirillum seropedicae SmR1] Length = 406 Score = 242 bits (618), Expect = 2e-65, Method: Compositional matrix adjust. Identities = 113/240 (47%), Positives = 155/240 (64%) Query: 12 YTYMHSVAVCALMIALGHQLGLSPEETRQAGIAGLLHDIGKMAVPADILNKPGKLTDDEF 71 YTY+HSVAVCALM+ALG LGL R+AG+ G LHD+GK +P ++LNKPGKL+D+EF Sbjct: 158 YTYLHSVAVCALMVALGRTLGLDETACREAGLGGFLHDVGKAFIPHELLNKPGKLSDEEF 217 Query: 72 TSVKKHAQAGHAILRGVDGIGDAALDVSLHHHEKIDGSGYPFNLKGNQISMVAKMGAVCD 131 + +KKH + G+ L + D A DV LHHHEKIDG+GYP L+ I++ ++M A+CD Sbjct: 218 SQIKKHPELGYKYLSQDPNVPDYARDVCLHHHEKIDGNGYPEGLRAGAITLYSRMAAICD 277 Query: 132 VYDAITSNRPYKPGWEPGHSVKRMASNQGHFDETILEAFIKSVGIFPTGSCVVMHSGKVG 191 VYDA+TS+RPYK W+P ++ MAS GHFD + AF+K++GI+P GS V + SG+ G Sbjct: 278 VYDALTSDRPYKRAWDPAEAISAMASWTGHFDRAMFLAFVKTLGIYPVGSIVKLESGRSG 337 Query: 192 IVIDQHPGSLLTPKVKVFFSALTMAPLPIEIIDLATTRSGDKIASHADPAKLHLPNLETI 251 VI Q+ L P +KVF +M EI+DL D I + L +L+ + Sbjct: 338 FVIRQNAQDLTRPVLKVFHYDDSMTVTDTEILDLLANPEADTIVARGGEEWARLSHLDAM 397 >gi|300313282|ref|YP_003777374.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 410 Score = 218 bits (556), Expect = 3e-58, Method: Compositional matrix adjust. Identities = 101/198 (51%), Positives = 136/198 (68%) Query: 12 YTYMHSVAVCALMIALGHQLGLSPEETRQAGIAGLLHDIGKMAVPADILNKPGKLTDDEF 71 YTY+HSV+VCAL+I LG LGLS R+AG++G LHD+GK + +ILNKPGKLT +EF Sbjct: 155 YTYLHSVSVCALVIGLGRTLGLSEAACREAGLSGYLHDVGKAFIDDEILNKPGKLTAEEF 214 Query: 72 TSVKKHAQAGHAILRGVDGIGDAALDVSLHHHEKIDGSGYPFNLKGNQISMVAKMGAVCD 131 +K+H + G+ L + A DV HHHE++DG GYP L I++ A+M AVCD Sbjct: 215 ALIKRHPELGYQYLIQTPDMPAYASDVCRHHHERLDGRGYPDALPAESITLYARMTAVCD 274 Query: 132 VYDAITSNRPYKPGWEPGHSVKRMASNQGHFDETILEAFIKSVGIFPTGSCVVMHSGKVG 191 VYDA+TS RPYK GW+P ++ +MA +GHFD +L AF+K++GI+P GS V + SG G Sbjct: 275 VYDALTSERPYKHGWDPAEAISQMARWEGHFDRRLLLAFVKTLGIYPVGSLVRLESGVSG 334 Query: 192 IVIDQHPGSLLTPKVKVF 209 +VI Q+P L P +KV Sbjct: 335 VVIRQNPADLTRPVLKVL 352 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 855,426 Number of extensions: 35515 Number of successful extensions: 108 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 105 Number of HSP's successfully gapped: 10 Length of query: 258 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 175 Effective length of database: 1,226,865 Effective search space: 214701375 Effective search space used: 214701375 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)