BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0215.0041.p (479 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314020|ref|YP_003778112.1| major facilitator superfamily p... 653 0.0 gi|300309872|ref|YP_003773964.1| major facilitator superfamily p... 280 2e-76 gi|300310323|ref|YP_003774415.1| methylenomycin A resistance pro... 129 5e-31 gi|300312966|ref|YP_003777058.1| major facilitator superfamily p... 112 6e-26 gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 108 1e-24 >gi|300314020|ref|YP_003778112.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 475 Score = 653 bits (1685), Expect = 0.0, Method: Compositional matrix adjust. Identities = 339/415 (81%), Positives = 375/415 (90%), Gaps = 1/415 (0%) Query: 4 PKSKTPAAHPVVIAGEGLPADKRAIAVITLLIAVGLATLDTAIANTALPVMARDLNATPA 63 P + P+ H +VIAGEGLP ++R A++ L IAVGLATLDTAIANTALP MA DL TPA Sbjct: 8 PGTAAPSPH-IVIAGEGLPPEQRGRAILALGIAVGLATLDTAIANTALPAMALDLQTTPA 66 Query: 64 ASVWIVTAYQLAMMVSLLPLAALGEIVGYRRIYIAGLALFTAASLLCAVAWSLPALTVAR 123 ASVWIVTAYQLAMMV+LLPLAALGEI+GYRRIYI GL LFTAASLLCAVAWSLP L +AR Sbjct: 67 ASVWIVTAYQLAMMVALLPLAALGEIIGYRRIYIWGLVLFTAASLLCAVAWSLPTLVMAR 126 Query: 124 FLQGLGGAGIMSVNTALIRYIYPTRSLGRGVGINTLIVAISFTVGPSVASGILAIAPWPW 183 FLQGLGGAGIMSVNTALIR+IYPTRSLGRGVG+NTLIVAI+FTVGPSVASGILAIAPWPW Sbjct: 127 FLQGLGGAGIMSVNTALIRFIYPTRSLGRGVGLNTLIVAIAFTVGPSVASGILAIAPWPW 186 Query: 184 LFAVNVPLGIVGLLMAIPSLPYTPKSSHHFDIKSALLNAAAFGLLIMAIGEGAHQAAPAV 243 LFAVNVPLG++ +++A+PSLP+T + H FD++SALLNAAAFGLLI+AIGEGAHQA +V Sbjct: 187 LFAVNVPLGVLAVVLAVPSLPHTSLARHAFDLRSALLNAAAFGLLILAIGEGAHQAQASV 246 Query: 244 VALEFVAALVCGYLLMRREASHPAPMLPVDLFRRPMFSLSAITAVCSFAAQGLAFVSLPF 303 V LE AA++CG L++RE SHPAPMLPVDLFR PMFSLSA+TAVCSFAAQGLAFVSLPF Sbjct: 247 VGLELAAAILCGLFLLKRELSHPAPMLPVDLFRHPMFSLSAVTAVCSFAAQGLAFVSLPF 306 Query: 304 FFHHDLGRSQVEIGLLMTPWPVAVAIMAPIAGRMSDHYPAGILGGFGLALLCVGLMLMTL 363 FFHHDLGRSQVEIGLLMTPWPVAVAIMAPIAGRMSD YP G+LGG GLALLC GL+ MT Sbjct: 307 FFHHDLGRSQVEIGLLMTPWPVAVAIMAPIAGRMSDRYPVGMLGGVGLALLCAGLLSMTF 366 Query: 364 MPAAPSVFEISWRMFVCGMGFGFFQSPNLKALMTSAPPNRSGGASGIVATARLFG 418 MPA+PSV+EISWRM +CGMGFGFFQSPNLKALMTSAPP+RSGGASGIVATARL G Sbjct: 367 MPASPSVWEISWRMLLCGMGFGFFQSPNLKALMTSAPPHRSGGASGIVATARLLG 421 >gi|300309872|ref|YP_003773964.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 493 Score = 280 bits (717), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 172/408 (42%), Positives = 251/408 (61%), Gaps = 8/408 (1%) Query: 19 EGLPADKRAIAVITLLIAVGLATLDTAIANTALPVMARDLNATPAASVWIVTAYQLAMMV 78 EGLP +R A++ ++ A +A D I N ALP +++ L + A S+W+ AY LA + Sbjct: 20 EGLPGKQRRRAMLAVMTATTMAVFDGTIINVALPQISQALQTSAAVSIWVSNAYLLATAM 79 Query: 79 SLLPLAALGEIVGYRRIYIAGLALFTAASLLCAVAWSLPALTVARFLQGLGGAGIMSVNT 138 +L AAL +G+R + AGLA+FT ASL CA++ S+ L + R LQGLGGA ++S+ Sbjct: 80 TLAAFAALSSRIGFRAQFSAGLAVFTLASLGCALSGSVTLLILMRILQGLGGAAMLSIAP 139 Query: 139 ALIRYIYPTRSLGRGVGINTLIVAISFTVGPSVASGILAIAPWPWLFAVNVPLGIVGLLM 198 A+ R I+P R LGR +G+N ++VA S VGP + +LA+ W W+FA+NVPLG++ + + Sbjct: 140 AIHRTIFPNRLLGRILGLNAVLVAASTAVGPVLGGTLLAMLGWEWIFAINVPLGVLAVWL 199 Query: 199 AIPSLPYTPKSSHH-FDIKSALLNAAAFGLLIMAIGEGAHQAAPA-------VVALEFVA 250 A ++P T K + FD+ AL +A A G LIMA A A P A V Sbjct: 200 AWNAVPDTRKPAQAAFDLAGALWSALAMGALIMAADTCARFAEPGQGSGAAFTAAAYAVT 259 Query: 251 ALVCGYLLMRREASHPAPMLPVDLFRRPMFSLSAITAVCSFAAQGLAFVSLPFFFHHDLG 310 AL+ G +R + P+LP+D+F P FSL+A+T++ SF QG+AFV+LPF F + G Sbjct: 260 ALLAGLAFVRGQRRAADPLLPLDIFANPRFSLAALTSLASFVGQGIAFVALPFLFQNAYG 319 Query: 311 RSQVEIGLLMTPWPVAVAIMAPIAGRMSDHYPAGILGGFGLALLCVGLMLMTLMPAAPSV 370 + E L TPWP+ + ++AP AGR++D + +L GLAL +GL+L+ L+P Sbjct: 320 YTAFESAALFTPWPLGIVLVAPHAGRLADRHSPALLSSLGLALFALGLVLLALLPDQAQP 379 Query: 371 FEISWRMFVCGMGFGFFQSPNLKALMTSAPPNRSGGASGIVATARLFG 418 ++I WR VCGMGFGFFQSPN + ++ + RSG ASG++A R FG Sbjct: 380 WDIGWRALVCGMGFGFFQSPNNREMLGNVSRERSGNASGVLAIMRTFG 427 >gi|300310323|ref|YP_003774415.1| methylenomycin A resistance protein [Herbaspirillum seropedicae SmR1] Length = 478 Score = 129 bits (324), Expect = 5e-31, Method: Compositional matrix adjust. Identities = 103/316 (32%), Positives = 153/316 (48%), Gaps = 6/316 (1%) Query: 30 VITLLIAVGLATLDTAIANTALPVMARDLNATPAASVWIVTAYQLAMMVSLLPLAALGEI 89 ++ L I +A LD + N L +A L+A + VWIV Y L LL AL + Sbjct: 32 LVALAIGFVMAMLDVTVVNVGLSNIAGSLDAPLSTLVWIVDGYTLTFAAMLLVGGALADR 91 Query: 90 VGYRRIYIAGLALFTAASLLCAVAWSLPALTVARFLQGLGGAGIMSVNTALIRYIYP-TR 148 G RR+Y+ GL LF ASLLC A + P L VAR LQGLG A M + +L+ ++Y R Sbjct: 92 FGARRLYLTGLFLFVLASLLCGAAPNGPFLIVARLLQGLGAAFFMPSSLSLLTHVYEDDR 151 Query: 149 SLGRGVGINTLIVAISFTVGPSVASGILAIAPWPWLFAVNVPLGIVGLLMAIPSLPYTPK 208 R +G+ + V ++ VGP V ++ W +F +NVP+G+VGL+MA +P Sbjct: 152 VRARMLGVWSATVGLAAAVGPLVGGVLIHWLGWRSVFLINVPVGLVGLVMARKLIPQVGG 211 Query: 209 SSHHFDIKSALLNAAAFGLLIMAIGEG--AHQAAPAVVALEFVAALVCGYLLMRREASHP 266 + ++ S L L + +G +P ++A AL G L+R E Sbjct: 212 HARALNLSSHLAGVVMLAGLSFVLIQGPVYGWTSPRILA-GLAVALSAGAALIRHELRGI 270 Query: 267 APMLPVDLFRRPMF-SLSAITAVCSFAAQGLAFVSLPFFFHHDLGRSQVEIGLLMTPWPV 325 AP+LP LF P F + + I + +F G F L F G ++ GL + P Sbjct: 271 APLLPKALFATPQFAAANGIGFLINFGVYGNLFY-LALFLQQGRGADALQTGLQLVPMMA 329 Query: 326 AVAIMAPIAGRMSDHY 341 + ++GRMS H+ Sbjct: 330 VIFFGNLMSGRMSAHW 345 >gi|300312966|ref|YP_003777058.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 544 Score = 112 bits (281), Expect = 6e-26, Method: Compositional matrix adjust. Identities = 114/394 (28%), Positives = 186/394 (47%), Gaps = 13/394 (3%) Query: 39 LATLDTAIANTALPVMARDLNATPAASVWIVTAYQLAMMVSLLPLAALGEIVGYRRIYIA 98 + LDT + LP +ARDL A+ + W+V++Y L LLP A + G RR+ + Sbjct: 47 MTVLDTNVVGIILPTVARDLGASFSQIEWVVSSYVLCFAALLLPAGATADRYGRRRVLLG 106 Query: 99 GLALFTAASLLCAVAWSLPALTVARFLQGLGGAGIMSVNTALIRY-IYPTRSLGRGVGIN 157 G+ALF SL CA+A S AL AR LQG+G A +++ A+I + + R + Sbjct: 107 GIALFALTSLACALAPSAQALYGARALQGVGAAFLLAPALAVIGHAFHDEDQRERAWALW 166 Query: 158 TLIVAISFTVGPSVASGILAIAPWPWLFAVNVPLGIVGLLMAIPSLPYTPKSSH----HF 213 I+ ++ + P + + + W W FAVN+P LL+A+ + P+S + Sbjct: 167 GGIMGLTMVLAPLIGGVVNTLLGWRWAFAVNLP---ACLLLAVAVWRHVPESRNPLPRPL 223 Query: 214 DIKSALLNAAAFGLLIMAIGEGAHQAAPAVVALE---FVAALVCGYLLMRREASHPAPML 270 D L +++A LL A+ G +V + A L+ ++ +R A+H PML Sbjct: 224 DPLGILSSSSAVFLLTWALITGPEHGWTSVACVARGLGGALLLVLFIAWQRRAAH--PML 281 Query: 271 PVDLFRRPMFSLSAITAVCSFAAQGLAFVSLPFFFHHDLGRSQVEIGLLMTPWPVAVAIM 330 + LFR F + A+ + LP F + G S + GL M P+ +A+ ++ Sbjct: 282 ELSLFRHAGFVAAVAAMFAYAASAQVMASLLPLFLQNARGLSAWQTGLAMLPFALAMLLL 341 Query: 331 APIAGRMSDHYPAGILGGFGLALLCVGLMLMTLMPAAPSVFEISWRMFVCGMGFGFFQSP 390 + R++ + + GL + +G +LM + + + M V G G G Sbjct: 342 PQVGSRLARWLSSPQMLALGLGVTALGNLLMIQAALSGAAAWTALGMAVLGSGGGLLNGQ 401 Query: 391 NLKALMTSAPPNRSGGASGIVATARLFGLFFFFS 424 KA+M + P R+G ASGI TAR G+ F+ Sbjct: 402 TQKAIMGNVPRERAGMASGISTTARFTGVLAGFA 435 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 108 bits (270), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 107/323 (33%), Positives = 160/323 (49%), Gaps = 24/323 (7%) Query: 22 PADKRAIAVITLLIAVG--LATLDTAIANTALPVMARDLNATPAASVWIVTAYQLAMMVS 79 P +RA+ I +A G + TLDT I NTALP MAR L +V AY L M S Sbjct: 48 PVTQRALLWI---VASGFFMQTLDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTM-AS 103 Query: 80 LLPLAA-LGEIVGYRRIYIAGLALFTAASLLCAVAWSLPALTVARFLQGLGGAGIMSVNT 138 L P + L + G RR+Y A + LF+ SLLCA+A SLP L AR +QGLGG+ ++ V Sbjct: 104 LTPASGWLADRFGTRRMYFAAILLFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGR 163 Query: 139 ALIRYIYPTRSLGRGVGINTLIVAISFTVGPSVASGILAIAPWPWLFAVNVPLGIVGL-- 196 + P + ++ + VGP + ++ W W+F +N+P+G++G+ Sbjct: 164 LAVLRAVPGAQYIAALASISIAGQLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGA 223 Query: 197 -LMAIP--SLPYTPKSSHHFDIKSALLNAAAFGLLIMAIGE--GAHQAAPAVVALEFVAA 251 L +P SL TP+ FD+ +L +A +A+ +H+ + +AL VAA Sbjct: 224 VLYFLPDDSLAGTPR----FDLLGCVLLSACMVAFSLALDVPFDSHRTGWS-IALFVVAA 278 Query: 252 L-VCGYLLMRREASHPAPMLPVDLFRRPMFSLS-AITAVCSFAAQGLAFVSLPFFFHHDL 309 L V GY R + HP P+ + LF P FS+ A VC + + F+ L Sbjct: 279 LTVLGY--WRHASVHPRPLFRLGLFGEPNFSIGLAGNLVCRIGSSAVPFLLP-LLLQVRL 335 Query: 310 GRSQVEIGLLMTPWPVAVAIMAP 332 G S + G+++ P +A I P Sbjct: 336 GYSPLHAGMVLLPAAIAGTIAKP 358 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.138 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,452,309 Number of extensions: 59124 Number of successful extensions: 417 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 400 Number of HSP's successfully gapped: 17 Length of query: 479 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 390 Effective length of database: 1,198,455 Effective search space: 467397450 Effective search space used: 467397450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)